Research Opportunity Program
BCH299Y: Molecular Evolution
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Research Opportunity ProgramBCH299Y: Molecular Evolution |
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The HSP70 Aligned Sequence DatabaseSequence alignment is the key to understanding molecular evolution. It's not as easy as you might imagine. Some genes are almost impossible to align. Fortunately for us, the HSP70 proteins are easy to align. We don't have to concern ourselves with the many problems and controversies concerning sequence alignment but there's a few things you should be aware of. The HSP70 Aligned Sequence Database is a multiple sequence alignment. This means that all of the available sequences are aligned in one large database. The alternative is pairwise sequence alignment where only pairs of sequences are aligned. The method I used in the HSP70 multiple sequence alignment is called "progressive alignment". This is just a fancy term for common sense. What it means is that when you introduce a gap to align any one sequence then this gap must be preserved in all the other alignments as you progress in building the multiple sequence alignment. In other words, "once a gap, always a gap". There are lots of examples in the HSP70 aligned sequence databases, such as the gap between positions 30 and 31 (see below). Sometimes this method produces complicated looking patterns such as the region between positions 72 and 100. There are computer programs that align sequences. Most of them will only handle pairwise alignments but these are very important in some applications such as searching the sequence databases. Other programs can make multiple sequence alignments from a number of sequences but none of them do as good a job as an experienced human being. If you're interested in seeing this for yourself I can show you some of these programs. This could be a project. It's hard to learn anything from looking at aligned sequences if every amino acid is shown. What we normally do is try to simplify the data in order to see the patterns. Look at the aligned sequences at the bottom of this page. Each dot on the page means that the amino acid at that position is the same as the one in the consensus sequence at the top of the list. It is much easier to see which sequences are similar if we eliminate the highly conserved regions by replacing them with dots. This is a purely cosmetic change. As you might guess, the consensus sequence is somewhat arbitrary. Each amino acid in the consensus sequence was chosen to represent the most common amino acid at that position but in some cases there is no clear choice. For example, I choose "Q" at position 29 because this is the amino acid found in almost all of the eukaryotic versions of HSP70. But I could just as easily have chosen "E", the amino acid present in the prokaryotic sequences. In some cases there's no obvious consensus so I've inserted an "X" in the consensus sequence (eg. position 278). There is no amino acid called "X". Parts of the sequence can't be aligned. The most obvious cases occur at the ends of the protein. At the beginning (N-terminus) there are often a few extra amino acid residues. This is a very common situation with homologous proteins. In the case of HSP70's there's an additional complication because some of the proteins have an N-terminal leader sequence. The BiP leaders are required to ensure that the protein is inserted into the lumen of the endoplasmic reticulum (ER). Read about the signal hypothesis in your textbook. The mtHSP70 leader sequence targets these proteins to the mitochondria. Similarly, the cBiP leader sequence indicates that this protein has to enter the chloroplast. All of the mBiP genes and most of the cBiP genes are found in the nucleus so the proteins are made in the cytoplasm and have to be transported across the organelle membrane. Note that some of the cBiP proteins in the database don't have a leader sequence. Can you guess why? The C-terminus also has extra amino acids and these are difficult to align. The HSP70's are particularly tough because this region is not highly conserved. I'm still working on this alignment so for now we'll ignore the last 50 amino acids when we construct a phylogenetic tree. There are a few internal regions that are difficult to align (around position 285, for example) but these aren't very important. The big advantage of HSP70's is that the alignment is very obvious - much more obvious than most other proteins. HSP70 is probably the most highly conserved protein known in biology. All BCH299Y students will learn how to construct multiple sequence alignments. Each student will be responsible for inserting some new sequences into the multiple sequence alignment database. |
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*NO1
*NO2 1 15 30 45 6O 75
*NO3 | | | | | |
*2nd CONSENSUS XAIGIDLGTTYSCVAVFQNGRVEIIANDQG----NRTTPSYVAFTDD-GERLIGDAAKNQAATNPENTVFDAKRLIGRRF
E. coli: hsc70 MALLQISEPGLSAAPHQRRL.A.......N.L..TVRS.QA.TL.DHE.----RHLL..V.HY-QQ-QGHSV.YTPRTN..LDTA..ISSV...M..SL
H. influenzae: hsc70 MALLQIAEPGQAAAPHQHRL.V.......N.L..SVRS.QSV.LNDE.E----RSLV..V.HY-GV-E.KKV.LE.FE..SLD.K...ISV......SL
Buchnera aph. hsc70 MVFLKNKVKKKLIL...F.....LL.SV..E..VLLTD.KK----RYLL..I.N.-NK-KKP...WE.EKKIIKD.I..ITSV......SI
B. megaterium: MSKI........N.....LEG.EPKV.P.PE.----......V...-KN-...QV.EV..R..I...-..IISV..HM.---
B.subtilis: dnaK MSKV........N.....LEG.EPKV...AE.----......V...-KN-...QV.EV..R.SI...-..IMSI..HM.---
Bacillus stearo. MSKI........N.....LEG.EPKV.P.PE.----......V...-KN-....V.EV..R..I...-...ISI..HM.---
Staph. aureus: MSKI........N...T.LEGDEPKV.Q.PE.----S.....V...-KN-..TQV.EV..R..I...-...QSI..HM.---
Streptococcus pyog. MSKI........N.A...LEGTESK....PE.----......V.S.-KN-..IIV.....R..V...-E..ISI.SKM.---
Streptococcus mutans MSKI........N.A...LEGTESK....PE.----......V.S.-KN-..IIV.....R..V...-E.ILSI.SKM.---
Streptococcus agal. MSKI........N.A...LEGTESK....PE.----......V.S.-KN-..IIV.....R..V...-D..ISI.SKM.---
Streptococcus pneu. MSKI........N.A...LEGTESK....PE.----......V.S.-KN-..IIV.....R..V...-D..ISI.SKM.---
L. lactis: dnaK MSKI........N.A...LEGTESK..P.PE.----......V...-KN-..IIV.....R..V...-E.IISI.SKM.---
Erysipelothrix: dnaK MSKV........N.A.S.MDG.EAKV.T.PE.----......V.S.-KN-...IV.....R.VV...-.SAVSV.....---
Mycoplasma: dnaK MAKEKI........N.V.S.IEG.QPI.LE.PE.----Q.....V...-KN-SDIIV.G...R..V...-.V.QSI.SKM.---
Mycoplasma pneum. MSTDNGLI........N...S.ME...PVVLE.PE.----K.....I.SY-KN-N.IIV.....R.MV...-..IVSI...M.---
Mycoplasma gen. MSADNGLI........N...S.MEG..PVVLE.PE.----K.....I.SY-KN-N.IIV.....R.MV...-..IVSI...M.---
Mycobacterium tuber. MAR.V.......N.V.S.LEG.DPVVV..SE.----S.....I...ARN-..V.V.QP.....V..VDR..RSV..HM.---
M. leprae: dnaK MAR.V.......N.V.S.LEG.DPVVV..SE.----S.....T...ARN-..V.V.QP.....V..VDR.IRSV..HM.---
M.paratuberculosis: MAR.V..RY...N.V...LEG.DPVVV..SE.----S.....I...ARN-..V.V.QP.....V..VDR.IRSV..HM.---
Streptomyces: dnaK MAR.V.......N.V.S.LEG.EPTV.T.AE.----A.....V...AKN-..V.V.EV..R..V..VDR.IRSV..HM.---
S. griseus: dnaK MAR.V.......N.V.S.LEG.EPTV.T.AE.----A.....V...AKN-..V.V.EV..R..V..VDR.IRSV..HM.---
Clostridium: dnaK MSKI........N.....MEG.EPVV.T.SE.----A.....V.S.QAN-....V.QV..R..I...DK.IMSI..HM.---
Clostridium aceto.: MSKV........N.....MEG.DPAV...SE.----A.....V.S.QKN-....V.QV..R.SI...DK.IISI..KM.---
Methanosarcina: MAKIL.......N.....MEG.EAVV.P.AE.----S.....V.G.SKK-..K.V.QV..R..IS..D...YSI..HM.---
Halobacterium: MASNKIL.......N.AF..MEG.DP...V.GE.----E.....V...-D.-....V.KP.....VK..DE.IQSI..HM.---
Halobact. cut.: MASEKIL.V.....N.AF..MEGSDP...T.EE.----DL....I..H-D.-..L.V.KP.....VQ..DQ.IASI..HM.---
Thermoplasma a.: MSKI.......SN.AA..VIS.KPTV.PSSE.VSIGGKAF.......K.-.QM.V.EP.RR..LL...G.I.A...KM.---
Synechocystis: dnaK1 MGKV........N..AS.LEG.KPIV.T.TE.----G.....I.G..KG-SQ..V.QL..R.SV..A....YSI..F....W
Synechococcus: dnaK1 MGKV........N.....LEG.KPI.VT.RE.----D.....I..VGRK-.D.IV.RM..R..V..A....YSI..F....W
Therm. aquaticus MAK.V.......N.VI..LEG.KPVVLE.AE.----E.V...V...-R.-..T.V.RM..R..VL...G.I.EI..F.....
Deinococcus prot.: .N.VI..MEG..P.V.V.AE.----......V..Y-KG-D...V.QI.RR...L..QA.L.EV..F....W
Thermomicrobium ros: MGRV........N.VM..IEG.EPVV.PTAE.----E.L...V..I-TR-....V.RF..R..I......IYSI..FM....
Synechocystis: dnaK2 MGKVV.......N.....MEG.KPTV...AE.----F.....V.GYAKN-.D..V.QI..R..VM..G..FYSV..F...K.
Synechococcus: dnaK2 MAKVV.......N.....MEG.KPTV...AE.----F.....V...AKN-QD..V.QI..R..VM.....FYSV..F....P
Synechocystis: dnaK3 MLTSINRSANFGSKENNMGKVV.......N.V...MEG.KPIV...AE.----M.....V.G.NKE-..LVV.QMGRR..VL..Q..FYGV..FM...Y
Synechococcus: dnaK3 MGRVV.......N.VI..MEG.KPMV...AE.----V.....V.GVSKT-....V.EL.RR.LVL..R..FANI..F....Y
Anabaena variabilis MGKVV.......N.V...MEG.KPVV...AE.----M.....V.G.SK.-...VV.QM.RR.TVL..Q..F.AV..Y....Y
Aphanothece halo. MGKV........N..F..LEG.KPLV.E.VE.----G.....I....KE-K...V.QL..R..V..A...IYSI..F....W
Porphyra: cBiP: MGKVV.......N.VI..MEG.KPTV.P.AE.----F...A.V..Y.KS-.DK.V.QI.-R..VI.....FYSV..F...KQ
Chlamy. rein. cBiP MPVQQMTSMRSQSLAGAPVAPVKAGRAGVSRRALAVSVRAEKVV.......N.A..AMEG.KPT..T.AE.----G.....V....KT-.D..V.QI..R..VV.....F.SV..F....M
pavlova: cBiP MAKVV.......N.V...MEG.KPTV.T.SE.----GT....V..YAKN-.DL.V.QI..R..VI.S...FYSV..F...PS
Odontella sin. cBiP MGKVV.......N.V..AIEG.QPSV.V.AE.----L.....I..Y.KK-Q.L.V.QI..R..VI.....F.SV..F..SKE
Cryptomonas: cBiP: MGKVV.......N.V...MEG.KPAV.Q.AE.----F.....V..Y.KT-.D..V.QI..R..VI..D..FYSV..F..W.S
Spinach: cBiP EASSVV.....F.SV..F...KM
Pisum sativum cBiP M*NSKTLFFGQRLNSNHSPFTRAAFPKLSSKTFKKGFTLRVVSEKVV.......N.A..AMEG.KPT..T.AE.----Q.....V..Y.KN-.D..V.QI..R..VV.....F.SV..F...KM
Cucumis sativ. cBiP M*SSRSVFFGQKLGNSSAFPAAAFLNLRSNTSRRNSSVRPLRIVNEKVV.......N.A..AMEG.KPT.VT.AE.----Q.....V..Y.KN-.D..V.QI..R..VV.....F.SV..F...KM
Cucumis sativ. mBiP M*SSRSVFFGQKLGNSSAFPAAAFLNLRSNTSRRNSSVRPLRIVNEKVV.......N.A..AMEG.KPT.VT.AE.----Q.....V..Y.KN-.D..V.QI..R..VV.....F.SV..F...KM
Citrullus lan. cBiP M*SSRTVFFGQKLGNSSAFPTATFLKLRSNISRRNSSVRPLRIVNEKVV.......N.A..AMEG.KPT.VT.AE.----K.....V..Y.KI-.D..V.QI.NR..VV..RD.F.SV..F...KM
Aquifex aeolicus MAEKKEKI........N.V.S.MMGDEAVV.Q.QE.----S.L...V.SW.KE-K.I.V.EP..RR.ILD.....YES..F...K.
C. pneumoniae: MSEHKKSSKI........N...S.MEG.QAKV.TSSE.----T.....I...-KG-N.K.V.IP..R..V....K.LGST..F...KY
Chlamydia: dnaK MSEKRKSNKI........N...S.MEG.QPKV..SSE.----T.....I...-KG-..T.V.IP..R..V....K.LAST..F...K.
Chlamydia pneumoniae MSEHKKSSKI........N...S.MEG.QAKV.TSSE.----T.....I...-KG-N.K.V.IP..R..V....K.LGST..F...KY
Borrelia: dnaK MGKI........N....IMEH.KPVV.Q.SE.----G.....I..Y.NK-....V.QV....MV......IYSI..FM....
Leptospira interrog. MSKEKI........N.V.S.MEG.DPVV.Q.SE.----A.....I....AK-..T...QF.....I..AV..IRS...F....L
Helicobacter pylori: MGKV........N.AM..YEGNEAKDLLRIKE---GKN....I.....K-..I.V.ES..R..V....K.IYSI..IM.LM.
Myxococcus xanthus MGKV........N.....MEG.EPVV.P.SE.----S.....M.G...S-....V.QI..R..I........A.......K.
Caulobacter: dnaK MSKI........N....IMDGKTPKV.E.AE.----A.....V...LE.-......QP..R..V...T..L.AI......TA
Rhizobium meliloti: MAKV........N...S.MDGKDAKV.E.AE.----A.....M....E.-....V.QP..R..V......L.AI......T.
Bradyrhizobium japo. MGKV........N.....MDGKNAKV.E.SE.----M.....I..V...-....V.QP..R..V....R.F.AV.......Y
Rhodoba capsulatus: MAKV........N....IMDGSQPRV.E.SE.----A.....I..Y-T.-N.R.V.QP..R..V...T....AV......R.
Agrobacter. tum.: MAKV........N.....MDGKDTKV.E.AE.----A.....M...S..-....V.QP..R..V...T..L.AV.......Y
Brucella: dnaK MAKV........N.....MDGKNAKV.E.AE.----A.....II....G-D.GVA.QP..R..V....G.L.AV...L...Y
Rhodopseudomonas sp. MGKV........N.....MDGKSAKV.E.AE.----M.....I..I...-....V.QP..R..V....R.F.AV.......Y
Rhizobium legum.: MAKV........N.....MDGKDAKV.E.AE.----A.....M...S..-....V.QP..R..V...T..L.AV.......Y
Pseudomonas cepacia: MGKI........N....IMEGNQ.KV.E.SE.----T.....II.YM..-N.V.V.AP..R.SV...K..L.AV......R.
Ralstonia eutropha MGKI........N...SILEGNTPKV.E.SE.----A.....II.YME.-..I.V.AP..R..V...R..LYAV......K.
Nitrosomonas europ. MAKI........N.....MEGNKPKV.E.AE.----A.....I..YAE.-N.I.V.AS..R..V......L.AI........
Franc. tularensis: MGKI........N..L.IMDGKTAKV.E.AE.----H.....V..Y..S-..I.V.Q...R..V...D..F.AI......KY
Legionella pneumo. MAKI........N.....MEGDKPKV.E.SE.----H.....I......-N.I.V.QS..R.SV....K.L.AI........
Haemoph. actinomyc. MGKIV.......N.....MEGEKPRV.E.AE.----D.....II.Y.N.-N.T.V.QP..R..V...K..L.AI........
Haemoph. influenzae: MGKI........N.....MDGDKARV.E.AE.----A.....II.Y..N--.T.V.QP..R..I...K..L.AI........
Haemoph. ducreyi: MGKI........N.....MDGDKPRVLE.AE.----A.....II.Y..K--.T.V.QP..R..I...K..L.AI........
Pasteurella haem. MGKI........N.....MDGDKPRV.E.AE.----A.....II.Y..K--.T.V.QP..R..I...K..L.AI........
Vibrio cholerae MGKI........N.....LDGDKPRV.E.AE.----E.....VI.Y..G--VS.V.QP..R..V...Q..L.AI........
Buchnera sp. MGKI........N....IMDGNKPRVLE.AE.----D.....II.Y.QE-..V.V.QP..R..I...K..L.AI......K.
Salmonella typh.: MGKI........N....IMDGTQARVLE.AE.----D.....II.Y.Q.-..T.V.QP..R..V...Q..L.AI........
E. coli: dnaK MGKI........N....IMDGTTPRVLE.AE.----D.....II.Y.Q.-..T.V.QP..R..V...Q..L.AI........
E. coli B: dnaK MGKI........N....IMDGTTPRVLE.AE.----D.....II.Y.Q.-..T.V.QP..R..V...Q..L.AI........
S. pombe: mBiP MISSRFTNVVRSGLRFQSKGASFKIGASLHGRMTARWNSNASGNEKVKGPV........T..L.IMEGQTPKV...AE.----T.....V....K.-....V.VS..R..VI.....F.AT........
yeast: mBiP MLAAKNILNRSSLSSSFRIATRLQSTKVQGSV........N.A..IMEGKVPK..E.AE.----S.....V....KE-....V.IP..R..VV.....L.AT........
yeast: Yel030p MLPSWKAFKAHNILRILTRFQSTKIPDAV........N.A..IMEGKVPR..E.AE.----S.....V....K.-....V.EP..R.SVI.S...L.AT........
Trich. vagin. hBiP FAQGMTPI........N...S.VEG.TPKV.Q.AE.----V.....I....NT-....V.EQ..R..V..SKS.F.AT......S.
Trich. vagin. mBiP MTPI........N...S.VEG.TPKV.Q.AE.----V.....I....NT-....V.EQ..R..I..SKS.F.AT.....CS.
Nosema locust. mBiP MCSVLCVYIYGSRAARLIRPFRKKFGHHKKMGAEVEKSTI........N...S.IKDRYPK..R.RT.----K.....T.T.-G.-----KIVVGSELVDGD.GA...GT......K.
Vairimorpha n. mBiP MTGKEISSRI........N...SLMH.NVPH..E.EE.----T.....V.S.-SK-DKI.V..Q..QNLTLF.K..I.AS......K.
Eimeria tenella mBiP M*KEQRVFSAVRTAAVGTLSSLAGRRGFSGVRGDVV.......N.....MEGSQPKVLE.SE.----M.....V....K.-.Q..V.VV..R..I......F.ST......S.
C. elegans: mBiP MLSARSFLSSARTIARSSLMSARSLSDKPKGHV........N...SIMEGKTPKV.E.AE.----V.....T....A.-....V.AP..R..V..SA..L.AT.......Y
Dros.: mBiP MLRVPKFLPRLARQAGVVPSHMSGASSMFRNLPGASNGISSQLRYKSGEVKGAV........N..L..MEGKQAKV.ENAE.----A.....H....K.-....V.MP..R..V..SA..FYAT........
Trypan. cruzi mBiP MFARRLRGAGSLAAASLARWQSSKVTGDV..............MEGDKPRVLE.TE.----F.A...V...-KG-Q.K.V.L...R..V...QS.F.AV........
T. cruzi: mBiP MFARRLRGAGSLAAASLARWQSSKVTGDV..............MEGDKPRVLE.TE.----F.A...V...-KG-Q.K.V.L...R..V...QS.F.AV........
Leishmania: mBiP MFARRVCGSAAASAACLARHESQKVQGDV..V..........TMDGDKARVLE.SE.----F.....V...-KG-S.K.V.L...R..I...QS.FYAV........
Crithidia: mBiP MFARRVCGTAAASAACLVRCASDKVQGDVI.V..........TMDGEKARVLE.TE.----F.T...V...-KG-N.K.V.L...R..I...LS.FYAV........
mouse: mBiP M*RPQDGWNGLSHEAFRFVSRRDYASEAIKGAVV.......N.....MEGKQAKVLE.AE.----A.....V....A.-....V.MP..R..V...N..FYAT..I....Y
Homo sapiens mBiP M*RHQDSWNGLSHEAFRLVSRRDYASEAIKGAVV.......N.....MEGKQAKVLE.AE.----A.....V....A.-....V.MP..R..V...N..FYAT.......Y
Rattus sp. mBiP M*RHQDGWNGLSHEVFRFVSRRDYASEAIKGAVV.......N.....MEGKQAKVLE.SE.----A.....V....P.-....V.MP..R..V...N..FYAT.......Y
Cricet. gris. mBiP M*RHQDGWNGLSHEAFRFVSRRDYASETIKGAVV.......N.....MEGKQAKVLE.AE.----A.....V....A.-....V.MP..R..V...N..FYAT.......Y
pea: mBiP MAATLLRSLQRRNLSSSSVSAFRSLTGSTKTSYATHKLASLTRFSSRPAGNDV........N...S.MEGKNPKV.E.SE.----A.....V...NQK-S.L.V.TP..R..V...T..L.GT........
bean: mBiP MAAVLRSLRRRDVASATFSAYRSLTGSTKPAYVAQKWSCLARPFSSRPAGNDV........N...S.MEGKNPKV.E.SE.----A.....V...NQK-..L.V.TP..R..V...T....GT........
potato: mBiP MATAALLRSLRRREFATSSISAYRTLASNTKPSWCPSLVGAKWAGLARPFSSKPAGNEI........N.....MEGKNPKV.E.SE.----A.....V...NQK-..L.V.TP..R..V...T..LSGT........
Lycopers. per. mBiP .L.V.TP..R..V...T..LSGT........
Spinacia oler. mBiP M*PYMAQRLASLVRPFSSRPAGNDV........N...S.MEGKSAKV.E.AE.----A.....V...NPK-..L.V.TP..R..V...T..I.GT......V.
Giardia: BiP MLALVFAALALAETI..............SRA.Q....P.EL.----A.V........A.-....V......Y.PIS....I..V......K.
yeast: BiP MFFNRLSAGKLLVPLSVVLYALFVVILPLQNSFHSSNVLVRGADDVENYGTV..............MK..KT..L..E..----..I.........--D...........V.A..Q..I..I.....LKY
K. lactis: BiP MFSARKSSVGWLVSSLAVFYVLLAVIMPIALTGSQSSRVVARAAEDHEDYGTV..............MK..KT..L..E..----..I.....S...--D.............S..K..I..I.....LQY
S. pombe: BiP MKKFQLFSILSYFVALFLLPMAFASGDDNSTESYGTV..............MK............----..I........E--D...V.E......PS.....I..I......K.
Achlya a.: BIP MLTSKVITAAALAACAFSSVAARRPKGPV............GHYA..K.........----..I......WDSN-D......G.....TI.....L..V......KY
Phaeodact. tr. BiP MMFMRIAVAALALLAAPSIRAEEAGEEAKMGTV............G..K..AL........----..I.......MPS-....V........TI........V......KY
P. falciparum: BiP MNQIRPYILLLIVSLLKFISAVDSNIEGPV............G..K......LN.EL.----..I.....S.V.--...KV.E...LE.TVH.TQ....V......K.
C. elegans: BiP MKTLFLLGMIAITAVSIYCKEEEKTEKKETKYETI............G.YK............----..I.......SG.Q.D..........LTI.....I.........DY
C. briggsae: BiP MKTLFLLGLIALTAVSVYCEEEEKTEKKETKYGTI............G.YK............----..I.......SGEQ.D..........LTI.....I.........DY
E. granul.: BiP: MGLSTYVGIFLLCILTLSRCKSGKEEYGTV............G..K............----..I.......SG.-............LTS..K..L.........DY
E. multiloc.: BiP: MGLSTYVGTFLLCILTLSHCKSGKEEYGTV............G..K............----..I.......SG.-............LTS..K..L.........DY
Aplysia: BiP MDRFTPFFLLVILFSSNLLVRADGDEEDEGDKKKSEVGTV............G..K....D.......----..I........A.-............LTS.....I..V......T.
Drosophila: BiP MKLCILLAVVAFVGLSLGEEKKEKDKELGTV............G.YK............----..I........A.-............LT................EW
chicken: BiP MRHLLLALLLGGARADDEEKKEDWGTVV...........G..K............----..I........PE-............LTS...............TW
hamster: BiP MKFPMVAAALLLLCAVRAEEEDKKEDVGTVV...........G..K............----..I........PE-............LTS...............TW
rat: BiP MKFTVVAAALLLLCAVRAEEEDKKEDVGTVV...........G..K............----..I........PE-............LTS...............TW
human: BiP MKLSLVAAMLLLLSAARAEEEDKKEDVGTVV...........G..K............----..I........PE-............LTS...............TW
tobacco: BiP (A) MAGGAWNRRTSLIVFGIVLFGCLFAFSIATEEATKLGTV............G.YK..H.........----..I...W.....--......E....L..V...R....V......K.
tobacco: BiP (B) MAGAWKRRASLIVFAIVLFGSLFAFSIAKEEATKLGTV............G.YK..H.........----..I...W.....--......E.......V...R.I..V......K.
barley: BiP MDRARGSALLLGVLLAGSLFALCAAKEEAKKLGTV............G.YK..H.........----..I...W.G...--......E.......V...R....V......K.
Giardia: hsc70 MATAP.V...........G.Y..EK......E..----AY..........--ADG....S....C.L.....I...........
Achlya ambisexualis: MDDATRKMKRLAIEEKINKARAEREEQALRAERGFSEV............G.WKD.Q......SE.----......W...NE--S.........L...S.AT........I...A.
yeast: hsc7OA1 MAEGVFQG..............T.ES-S......E..----..V...F....P--E.............L..R..............
Kluyveromyces: hsc70 MAEGVFPG..............TYE.-S......E..----..V...F....P--E.............L..K..............
yeast: SSA1 MSK.V............H.A.D..D.......----......F.....--T.............M..S............N.
yeast: SSA2 MSK.V............H.S.D..D.......----......F.G...--T.............M..A............N.
yeast: SSA3 MSK.V............H.S.D..........----...
yeast: SSA3 MSR.V............H.S.D..........----............--T.............I..H............K.
yeast: SSA4 MSK.V............H.A.D..........----............--T.............M..H............K.
Hansenula: hsp70 MSK.V............H.V.D..........----......F.....--T.............M..A............K.
Ustilago: MTK...............W..D...V......----............--S...........V.M..H............K.
Cladosporium: hsp70 MAP.............GIYRDD.I........----......F.....--T......S....V.I..H............K.
Blastocladiella: hsp MTTKLDSPAVGIGT*PLRSCCRGGPDRRRQLHPHPPSRARRSPAIPSLPHRL.........G.W..D..........----........G...--S...........V.M..H..............
Bursaphel.: ...V.M..Q............K.
Entamoeba: hsc70: MSKYTGP.V...........GIW..D..........----............--T...........I.M.VK..............
Trich. vagin. MS.............G..RHN..D.L...M.----...........E--D...V.........M..Q........M...K.
T. brucei: hsp7OA4 MTYE.............G.W..E..........----............--S...........V.M..T............K.
T. brucei: hsc70 MTYEG.............G.W..E..........----..........VN--N.V.V.....SH..RGSNGVI.........K.
T. cruzi: hsp7OA8 MTYEG.............G.W..E..........----............--S...........V.M..R............K.
L. major: hsc70.4 MSSTN..A..........G..K.EQVD.......----...........E--T...NRRC....V.M..S........M...K.
L. amazonensis: A MTFDG.............G.W..D..........----............--S...........V.M..H............K.
L. amazonensis: C MTFDG.............G.W..D..........----............--S...........V.M..H............K.
L. amazonensis: ......V.M..S......A-V...K.
Leishmania: hsp7OA3 MTFDG.............G.W..E.LD.......----............--S...........V.M..H............K.
Leishmania: pseudo MSSTKHR..........G.\.E\S.D.......----...........E--T.......\...V\M..\.....\..MI..K.
L. donovani: hsc70 MTFDG.............G.W..E..D.......----............--S...........V.M..H............K.
Theileria: MTGP...............YKDNN....P....----............--T...........E.R.....I.........K.
Theileria parva MTGP...............YKDNN....P....----............--T...........E.R.....I.........K.
P. cynmolgi: hsp70A2 MASGKASKPNLPESNI.............G.WR.EN.D.......----............--T...........V.R...............K.
P. falcip.: hsp70A MASAKGSKPNLPESNI.............G.WR.EN.D.......----............--T...........V.R...............K.
Eimeria: hsc70 MSEAP.V...........G.WK.DG.........----............--T...V.......V.R...............K.
Euplotes eurystomus MKGS.............G.WV.DK.........----...........E--T...........T.R..T............K.
Cryptosporidium par. MTSSEGP.............G.WR.DT.D.VP....----...........E--T...........V.R...............K.
Babesia microti MSQGP.............G.WK.ET....S....----............--V...V....T..D.R...............KI
Chlamy.: hsc70: MGKEAP.............GLW..D..........----............--T...........V.M..RH...........K.
Bremia: hsp7O MAQSVSV............G.W..D..........----............--T...........V.M.AH.......P....K.
Achlya: hsc70 MSGVQGASV...........G.W..D..........----............--S...........V.M..A............K.
Pyrenomonas: hsp70 MENKIEGV.............G.WLHD...V......----...........E--T......S....V.M..D..............
Ascophyllum nodosum MS.V...........G.W..D..........----....T......E--T...........T.M.T....Y........K.
Guillardia th. hsc70 PSA..........GIW..E..........----......W....E--S.............M..H..I.........KM
carrot: hsp70: MASKGGK.............G.W..D..........----.......FA...--TS......-...V.M..S..............
petunia: hsp7O MAGKGEGP.............G.W.HD..........----........G...--T...........V.M..I..............
tomato: hsc70A MAGKGEGP.............G.W.HD..........----........G...--T...........V.L..I..............
tomato: hsc70B MAGKGEGP.............G.W.HD..........----........G...--S...........V.M..I..............
Arabidopsis: 3 MAGKGEGP.............GGWQ
Arabidopsis: 2 MAGKGKGP.............G.W.HD..........----............--S...........V.M..V..............
Arabidopsis: 1 .......G.W.HD..........----............--S...........V.MS.V..............
spinach: hsc70 MAGKGEGP...........R.G.W.HD..........----............--S...........V.M..I..............
potato: hsp70 MAGKGEGP.............G.W.HD..........----........G...--S...........V.M..I....
soybean: hsp70 MATKEGK.............G.W..D.....P....----............--T...........V.M..Q..............
potato: hsp70DK MAKSEGK.............G.W..D.....P....----............--T...........V.M..Q..............
maize: hsp7OA1 MAKSEGP.............G.W.HD..........----........G...--T...........V.M..T..............
maize: hsp7OA2 MAKGEGP......E......G.W.HD........?.----............--T..........?V.M..T....
lily: hsp70 MAGKDEVP.............G.W.HD..........----............--T...........V.M..T............H.
sea urchin: hsp70 MESGP.............G.....K.........----............--S...........V.M..T..I...........
sea urchin: hsp70-4 MESGP.............G.....K.........----............--S...........V.M..T..I...........
Trich. britovi mBiP? MSKN.............G...H.K.........----..........-T.-T.......Q...V.L..H..............
B. malayi: MSKN.............G..MH.K.........----............--T...........V.M..H......N.....K.
C. elegans: hsp7OA MSKHN.V...........G..MH.K.........----............--T...........V.M..H............K.
Dros.: hsc70-4 MSKAP.V...........G...H.K.........----............--T...........V.M..TQ.I.........K.
D.sim.: hsc7O-1 MPKLP.V...........G...H.K.........----...........E--S...........V.M..N..I...........
Dros.: hsc70-1 MPKLP.V...........G...H.K.........----...........E--S...........V.M..N..I...........
Dros.: hsc7O-2 MGKIP.............G.W..SK.........----..........NE--T......P....V.M.AK............K.
mosquito: hsp70-1 MPS.............G...H.K.........----..........S.--T...........V.M..T............K.
mosquito: hsp70-2 MPS.............G...H.K.........----..........S.--T...........V.M..T............K.
mosquito: hsp70-3 MPS.............G...H.K.........----..........S.--T...........V.M..T............K.
Dros.: hsp7OA2 MP.............G.Y.H.K.........----............--S......P....V.M..R............KY
Dros.: hsp7OA4 MP.............G...H.K...N.Y...----............--S...N.EP....V.M..R............KY
Dros.: hsp7OA5 MP.........
Schistosoma: ..H...........----............--S......G....V.M..T......T.......
Hydra m.: hsp70 MSKAP.............G...H.K......E..----............--T...........V.M..S............K.
sea urchin: MAKAP.V...........G...E.K.........----............--T..........KT.S..YRSL-........N.
trout: 7O.14 MSKGP.V...........G...H.K.....M...----............--S...........V.M..C..............
salamander: hsp70 MSAPKGV.F...........G...H.K......N..----............--S.....AP....VSL..Q............K.
rat: hsc7OA MSKGP.V...........G...H.K.........----............--T...........V.M..T..............
mouse: hsc7OA MSKGP.V...........G...H.K.........----............--T...........V.M..T..............
hamster: hsc70A MSKGP.V...........G...H.K.........----............--T...........V.M..T..............
cow: hsc70 MSKGP.V...........G...H.K.........----............--T...........V.M..T..............
human: hsc7OA MSKGP.V...........G...H.K.........----............--T...........V.M..T..............
mouse: pseudo2 NS..R.G..RH..
human: pseudo1 MSKGP.V...........G..HQ.K.........----./.I....T.M.--T....S..S...V.M..T.V.......F.H..
chicken: MSGKGP.............G...H.K.........----............--T...........V.M..T..I.........KY
mouse: hsc7OB MSARGP.............G...H.K.........----............--T...........V.M..T..I..R......K.
human: hsc70B MSARGP.............G...H.K.........----............--T...........V.M..T..I.........K.
cow: hsp70 MSARGP.............G...H.K.........----............--T.....T.....V.M..T..I.........K.
Xenopus: hsp7OA1 MATKGV.V...........G...H.K.........----............--T...........V.M..Q............K.
Xenopus: hsp7OA2 MATKGV.V...........G...H.K.........----............--T...........V.M..Q............K.
mouse: hsc70C MAANKGM.............G...H.K.........----............--T...........V.M..Q............K.
mouse: pseudo? MAANKGM.............G...H.K.....DY.V----.....D......--TS...ER-S...V.M..Q............K.
human: hsp70B MQAPREL.V...........G...Q.....L.....----............--T...V.........L..H............K.
???????????? MAKNT.............G...H.K.........----............--T...........V.L..Q............K.
pig: hsp70com: MAKSV.............G...H.K.........----......S.....--T...........V.L..Q............K.
cow: hsp70A1 MAKNT.............G...H.K...
mouse: hsp7OA1 MAKNT.............G...H.K.........----............--T...........V.L..Q............K.
mouse: hsp70A2 MAKNT.............G...H.K.........----............--T...........V.L..Q............K.
cow: hsp70A1 MAKNT.............G...H.K...
human: hsp7OA1 MAKAA.V...........G...H.K.........----............--T...........V.L..Q............K.
human: hsp7OA2 MAKAA.............G...H.K.........----..
human: hsp70A* MAKAA.............G...H.K.........----..
CONSENSUS XAIGIDLGTTYSCVAVFQNGRVEIIANDQG----NRTTPSYVAFTDD-GERLIGDAAKNQAATNPENTVFDAKRLIGRRF
T T T C T à C à
*NO1
*NO2 8O 1OO 115 13O 145 16O 175 19O
*NO3 | | | | | | | |
*2nd CONSENSUS NDPEVQSDIKHVPFKVVND-DGKPDIQVEXKG-----ETKTFTPEEISAMVLTKMKETA-EAYLGKK--VTNAVVTVPAYFNDAQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKDX----GEKNILVF
E. coli: hsc70 --ADI.QRYP.L.YQFQAS-EN-GLPMI.TAA-------GLLN.VRV..DI.KALAAR.-TEA.AGE--LDGV.I......D.....G....ARL...H...LL.............SGQ------.GV.A.Y
H. influenzae: hsc70 --SD...RYSSL.YEF.AS-EN-GLPLIITAQ-------GAKS.I.V.SDI.SRLNHI.-.QR..GE--LSGV.I......D.....S....ARL.......LL.......L.....SGQ------.GI.A.Y
Buchnera aph. hsc70 --DFIKKEFPIL.YVIEDN-KD-GG.LFHTNS-------GLV..IDVTSEI.KFL..KS-CEFFN..--IDAT.I......DNL..DSI.K.AIS.KI.LI.LL....S..V....QLNT------.GLVV.Y
B. megaterium: -----------------------T.HK..AE.-------TQY..Q.M..II.QHL.GY.-.E...EP--..K..I.........E........K....E.E..........L....E.T.-----EDQTV..Y
B.subtilis: dnaK -----------------------T.YK..IE.-------.DY..Q.V..II.QHL.SY.-.S...ET--.SK..I.........E........K....E.E..........L......T.-----EDQT...Y
Bacillus stearo. -----------------------T.YK..IE.-------.KY..Q....II.QYL.SY.-.D...EP--..R..I..................R....E.E..........L......EE------DQT...Y
Staph. aureus: -----------------------T.YK.DIE.-------.SY..Q.....I.QNL.N..-.S...E.--.DK..I.........E........K....E.E..........L......T.-----KDEKV...
Streptococcus pyog. -----------------------TSEK.SAN.-------.EY..Q.....I.QYL.GY.-.D...E.--.EK..I..................K....E.E..V.......L...M..T.-----KDEK....
Streptococcus mutans -----------------------TSEK.SANA-------.EY..Q.....I.QYL.GY.-.D...E.--.EK..I..................K....E.E..V.......L......T.-----KDEK....
Streptococcus agal. -----------------------TSEK.SAN.-------.EY..Q.....I.QYL.GY.-.D...E.--.EK..I..................K....E.E..V.......L...M..T.-----KDEK....
Streptococcus pneu. -----------------------TSEK.SAN.-------.EY..Q.....I.QYL.GY.-.D...E.--..K..I..................K....E.E..V.......L......T.-----K.EK....
L. lactis: dnaK -----------------------TSEK.SAN.-------.EY..Q.....I.QNL.A..-.S...E.--.EKA.I..................K....E.E..V.......L......T.-----KDEK....
Erysipelothrix: dnaK -----------------------TGEK.TLE.-------.DY........I.GY..SY.-.D...E.--..K..I..................K....E.E..........L.F.I..T.-----K.EKV...
Mycoplasma: dnaK -----------------------TTSK.NLE.-------.DYS..Q...EI.RY..NY.-..K..Q.--..K..I...........K...........Q.E..........L......Q.------KET...Y
Mycoplasma pneum. -----------------------TSNK.TV.N--PDGS..EL....V..QI.SYL.DY.-.KKI..T--ISR..I.........E.N...T..K......E..........L...I..SN-----R.MKV..Y
Mycoplasma gen. -----------------------TSNK.KVQN--ADGT..ELS..QV..QI.SYL.DF.-.KKI...--ISR..I.........E.N...T..K......E..........L...I..AS-----R.MKV..Y
Mycobacterium tuber. -----------------------S.WSI.ID.-------.KY.AP....RI.M.L.RD.-.....ED--I.D..I.T................Q.........V.......PG.....GE-----K.QR....
M. leprae: dnaK -----------------------S.WSI.ID.-------.KY.AQ....R..M.L.RD.-.....ED--I.D..I.T.............E..Q.........V.......L......GE-----R.QT....
M.paratuberculosis: -----------------------T.WSI.ID.-------.KY.AQ....R..M.L.RD.-.....ED--I.D..I...............E..Q.........V.......L......GE-----K.QT....
Streptomyces: dnaK -----------------------T.WK.NLD.-------.D.N.QQ...F..Q.L.RD.-.S...E.--..D..I.........E.....E..E.........V.......L......DE-------QV....
S. griseus: dnaK -----------------------T.WKIDLD.-------.S.N.QQM..FI.Q.L.RD.-.S...E.--..D..I........SE.....E..E.........V.......L......D.-------QT....
Clostridium: dnaK -----------------------T.YK.NID.-------.DLS.Q.....I.Q.L.AD.-.....E.--..E..I.........E........R....D.KT........SL......M.-----SAHK...Y
Clostridium aceto.: -----------------------TAEK.AIDD-------.NY..Q.....I.Q.L.AD.-.....ET--..Q..I........S.........K....E...........SL......M.-----TNQK...Y
Methanosarcina: ---------------------E-ANYK.TLN.-------.DY..Q.....I.Q.L.AD.-.....ET--IKQ..I........S.........A....E...........SL......G.-----IDQK...Y
Halobacterium: ---------------------Q-D.YS..LD.-------EEY...QV...I.Q.I.HD.-.E...DE--IEK..I........R.........K...FE.E..V.......M.....DES------DQTV..Y
Halobact. cut.: ---------------------E-E.YT.ALG.-------DEY.......RI.Q.I.RD.-.E...QD--.EK..I........R.........E...FD.E..V......SM.....EDR------DQTV..Y
Thermoplasma a.: -----------------------T.YKFKVFD-------.E...QQ...FILQ.I.KD.-..F..EP--.NE..I........N.............FD.K..........L...V..SG-----KSEK....
Synechocystis: dnaK1 --DDTVEERSR..YNC.KG-RD-DTVS.SIR.-------QSY..Q.....I.Q.L.ADS-..F..EP--..Q..I......T................E............L......QE-----T.EL....
Synechococcus: dnaK1 --EDTEAERSR.TYTC.PG-KD-DTVN.TIRD-------RFC..Q.......Q.LRQD.-.TF..EP--..Q..I......T...........A....E....V.......LS.....LH-----ENSR....
Therm. aquaticus --E...EEA.R..Y...PG-PD-GGVR..V..-------.LY........I.R.LV.D.-SKK..E.--I.K..I.......N...E..AN..R....E............L.......G------NETV...
Deinococcus prot.: --D..KDEAARS..T.KEG-PD-GSVRI.VD.-------.DYA..QV..E..R.LVAN.-S.K..Q.--I.D..I......DNS..E...Q..E........V........L....ER.G------DET....
Thermomicrobium ros: D.....RT..L..YQ.RRA-QN-GGVA.KMGE-------RSY..Q.....I.Q.L.QD.-.....ET--.DK..I......D.S..N......R....E.R........S.L.......G------RGEGG.Y
Synechocystis: dnaK2 --D.ITNEATE.AYS..K.-GN-GNVKLDCPA-----QG.Q.A......Q..R.LVDD.-SK...ET--..Q..I........S.........K...IE...........SL........------NET....
Synechococcus: dnaK2 --D..TNELTE.AY..DTS-GN--AVKLDSSN-----AG.Q.A......Q..R.LA.D.-SK...ET--..Q..I........S.........K....E............L.......S------NER....
Synechocystis: dnaK3 --TDLTPES.R.AYTIRR.-.R-DN.K.RCPR-----LK.D.A.......I.R.LA.E.-SR...E.--..G..I........S.....R...K....E...........SL.....QGR------IQK....
Synechococcus: dnaK3 --D.LTDES.R..YT.RR.-PE-GNVRIVCPQ-----LSRE.A...VA..I.R.LA.E.-SR...EP--..G..I........S.....R...R....E.K..L......SL.....RR.------NQT....
Anabaena variabilis --N.LSPES.R..YTIRK.-.V-GN.K.ACPR-----LN.E.AA........K.LADD.-S....AA--..G..I........S.....R...R....E....L......SL.....RG.------TET....
Aphanothece halo. --EDTEQERNR.SYHC.PG-RD-KTVD.KCW.-------.QY..Q.L...I.QTL.AG.-....NET--..E..I......T..................M..........L.F....QE-----QREQV..Y
Porphyra: cBiP: --N.ISQE.RQTSYN.KTS--.-SS.KI.CPA-----LN.D.A......Q..R.LV.D.-ST...ET--..Q..I........S.........K....D...........SLS.....QN------NET....
Chlamy. rein. cBiP --S..G.ESTQ..YR.IE.--.-GNVKIKCPN-----AG.D.A......Q..R.LT.D.-AKF.ND.--.EK..I........S.........K....E...........SL...F...A------NET....
pavlova: cBiP --K..SDELRQTPY.IEDS--E-GK.RLKCPN-----LN.N.AA.....Q..R.LVND.-NK...E.--.EK..I........S.........K....E...........SL........------NET....
Odontella sin. cBiP --L.ISA.S.KL.Y...K.-QN-GN.KINCSS-----LN.E.S......Q..R.LIND.-TS...QD--..Q..I........S.....M...K...VE...........SL.......Q------NET....
Cryptomonas: cBiP: --E..SEEL.Q.SYI.KT.-SN-GN.KLDCPS-----LK.E.AS.....E..R.LVDD.-SK...ES--.KQ..I........S.........R....E...........S........N------NET....
Spinach: cBiP --T..DEES.Q.SYT..R.-EN-NN.KL.CPA-----IG.Q.AA.....Q..R.LVDD.-SKF.ND.--..K...........S..T......R....E...........SL...FE..N------NET....
Pisum sativum cBiP --S..DEES.Q.SYR.IR.-.N-GNVKLDCPA-----IG.S.AA.....Q..R.LVDD.-SKF.ND.--..K...........S..T......R....E...........SL...FER.N------NET....
Cucumis sativ. cBiP --S..DEES.Q.SYR.ER.-EN-GNVKL.CPA-----IG.Q.AA.....Q..R.LVDD.-SKF.ND.--..K...........S..T......R....E...........SL...FE..S------NET....
Cucumis sativ. mBiP --S..DEES.Q.SYR.ER.-EN-GNVKL.CPA-----IG.Q.AA.....Q..R.LVDD.-SKF.ND.--..K...........S..T......R....E...........SL...FE..S------NET....
Citrullus lan. cBiP --S..DEES.Q.SYR..R.-EN-GNVKL.CPA-----IG.Q.AA.....Q..R.LVDD.-SKF.ND.--..K...........S..T......R....E...........SL...FE..S------NET....
Aquifex aeolicus --E..KEEA.R.SY...P.-EK-G.AAFDIPN-----AG.LVR...VG.H..R.L..A.-..F..EP--.KK..I.......ER.........K....E.V..L.......M....H...------NVR...Y
C. pneumoniae: --S..A.E.QT..YT.TSG-SK-G.AVF.VD.-------.QY.....G.QI.M......-.....ET--..E..I........S..AS.....R....D.K...P..........I..VG------D.K.A..
Chlamydia: dnaK --S..E.E..T..Y..APN-SK-G.AVFDVEQ-------.LY.....G.QI.M......-.....ET--..E..I........S..AS.....R....D.K...P......L...I..EG------D.K.A..
Chlamydia pneumoniae --S..ASE.QT..YT.TSG-SK-G.AVF.VD.-------.QY.....G.QI.M......-.....ET--..E..I........S..AS.....R....D.K...P......L...I..VG------D.K.A..
Borrelia: dnaK --E..A.E..M..Y.IEKG-LN-G.AR.NISN-----IK.QMS.P....AT........-.....E.--..E..I..................K....E.K..V.......L...IE..H------.EIVA.Y
Leptospira interrog. --N.CE.EM...SY..IRS-GN-EGVKF.TSA-------GE...Q....R..M...Q..-.D...Q.--..K..I........E.........R....E.E..........L...F...N-----VNSK.A.Y
Helicobacter pylori: .EDKAKEAE.RL.Y.I.DR--N-GACAI.IS.-------.IYT.Q....KI.M.L..D.-.S...ES--..E..I........S........................S..LL......E------SEK.M.Y
Myxococcus xanthus DS..GKKA.GVS....ASS-PN-G.AW..IR.-------.GYS.P.V..I..M...Q..-.D...EQ--.SE..I........S.........R....S............L......VQ---DG.TER.A.Y
Caulobacter: dnaK S..V.EK.KGM..YRSSRA-RA-G.AW.KAH.-------.DYS.Q.V..FI.Q....A.-..H..EP--..K..I..................K....E............L......N.------G.K.A.Y
Rhizobium meliloti: E..TT.K.KGM..Y.I.KA-.N-G.AW..AH.-------TSYS.S.I...I.Q......-.S...E.--.EK..I..................K....D............L.......E------G.T.A.Y
Bradyrhizobium japo. D..M.EK.K.L..Y.I.KA-SN-G.AW..AD.-------Q.YS.SQV..FI.Q......-..H..Q.--.DQ..I..................K....E............L......TK------TGT.A.Y
Rhodoba. capsulatus: T.A..EK.K.L..YNI.DG-GN-G.AW..VR.-------EK.S.A.V..VI.Q......-.S...ET--..Q..I..................K....E.L..........L........------S.T.A.Y
Agrobacter. tum.: E..T.EK.KAL...EI.KG-.N-G.AW.KAQD-------.NYS.SQ....I.Q......-.S...E.--.EK..I..................R....D............L.......E------G.T.A.Y
Brucella: dnaK D..M.TK.KDL..Y.I.KG-.N-G.AW..VH.-------.KYS.SQ....I.Q......-.S...ET--..Q..I..................K....E............L......SE------G.T.A.Y
Rhodopseudomonas sp. D..M.EK.KGL..Y.I.KA-SN-G.AW..AD.-------..YS.SQV..FI.Q......-..H..Q.--.DQ..I..................K....E............L......AK------TGT.A.Y
Rhizobium legum.: E..T.EK.KHL...TI.KG-.N-G.AW..AN.-------.GYS.AQ....I.Q......-.S...E.--.EK..I.........RPGQPRMPAASL..E............L.......E------G.T.A.Y
Pseudomonas cepacia: EEK...K..GLM.YSIIKA-.N-G.AW..GH.------E.LMA.P.V..EAVR...K..-.D...EP--..E..I........S.........R....E.K..........L.F....AE----K.DRK.A.Y
Ralstonia eutropha EEK...K..GLM.YAI.KA-.N-G.AW.GVRD-------QKLA.PQV..EAVR...K..-.D...EP--..E..I..........C.......R....D.K..........L.F.M..NE----K.DRK.A.Y
Nitrosomonas europ. DEEV..K..SVT.Y.I.RA-.N-N.AWI.AR.-------RKIA.P.V..Q..I...K..-.D...EP--..E..I........S.........R....E.K..........L.F.....E-----.DRK.A.Y
Franc. tularensis: D.KA..E...*..YA.IKA-.N-G.AW.AT.E.------.KMA.PQV..E..R...K..-.D...EP--..E..I........S.........K....E.K..........L...V.S.K-----..QTVA.Y
Legionella pneumo. D..I..K...M..Y.IMKA-.N-G.AW.RV.D-------QDKA.PQ...E..R...K..-.D...EE--.KE..I........S.........R....E.K..........L...M...R-----.DSV.A.Y
Haemoph. actinomyc. E.E..KR..DIM..AITKA-.N-G.AW..V..-------EKLA.PQ...E..K...KA.-.D...EP--..E..I..................R....D.K..........L......GT-----.N.T.A.Y
Haemoph. influenzae: ESE...R...IM..EITRA-.N-G.AW.NV..-------DKLA.PQ...E..K...K..-.DF..ES--..E..I..............I...K....D.K..........L.F..GSSK-----ENQV.A.Y
Haemoph. ducreyi: T.N...R..EIM..EIAKA-.N-G.AW.NV..-------DKLA.PQ...E..K...K..-.DF..EP--..E..I..................R....E.K..........L.....S.K-----ENQI.A.Y
Pasteurella haem. T.A...R..EIM..EISKA-.N-G.AW.TV..-------DKLA.PQ...EI.K...K..-.DF..EP--..E..I..................R....D.K..........L.....S.K-----ENQT.A.Y
Vibrio cholerae E.E...R...IM.Y.I.KA-.N-G.AW..A..-------QKMAAPQV..E..K...K..-.DF..EP--..A..I...........P.....CR....E.K..........L......PG-----.DRT.A.Y
Buchnera sp. K.D...R...IM.YNI..S-.N-G.AWIDV.K-------QKMA.PQ...E..K...K..-.D...ET--IKE..I..................R....E.K..........L......GK-----.NRTIA.Y
Salmonella typh.: Q.E...R.VSIM.Y.IIGA-.N-G.AWLDV..-------QKMA.PQ...E..K...K..-.D...EP--..E..I..................R....E.K..........L......EV-----GNRT.A.Y
E. coli: dnaK Q.E...R.VSIM...IIAA-.N-G.AW..V..-------QKMA.PQ...E..L...K..-.D...EP--..E..I..................R....E.K..........L......GT-----.NRT.A.Y
E. coli B: dnaK Q.E...R.VSIM...IIAA-.N-G.AW..V..-------QKMA.PQ...E..K...K..-.D...EP--..E..I..................R....E.K..........L......GT-----.NRT.A.Y
S. pombe: mBiP KE....R...E..Y.I.EH-SN-G.AWL.AR.-------..YS.SQ.GGFI.S..R...-ST....D--.K............S......A..A........VV.......L.......N------DAIVA..
yeast: mBiP E.A...R...Q..Y.I.KH-SN-G.AW..AR.-------Q.YS.AQ.GGF..N......-......P--.K............S.........Q.V......VV.......L....E.S.------S.VVA..
yeast: Yel030p E.A...R..NQ....I.KH-SN-G.AW..ARN-------R.YS.AQ.GGFI.N......-....A.S--.K......................Q.I......VV.......L......SE------PKV.AV..
Trich. vagin. hBiP E.EMTKKCREM..YQIIKA-KN-G.AW.KDEK------GNEYS.AQ.G.F.........-.....TT--.KD..I........S.........K......M.T........L...TER.Q------GQTVA..
Trich. vagin. mBiP D.EMTKKCREM..YQI.KA-KS-G.AW.KDEK------GNEYS.SQ.G.F..M......-.....TS--.KD..I........S.........K......M.TL.......L...TER..------GQTVA..
Nosema locust. mBiP E...I.KY.QKL.Y.T.SH-VN-G.AWIKVSD-------RM.S.SQ.A.YI..EL.RC.-.DF.KSP--.SKS.I........S.........RL...K...V........L....GRTE------NGT.A..
Vairimorpha n. mBiP D.L.LKEYL.NL.YDTTRH-CN-G.VWIKIDE-------.KYS.AQ.G.FI.S...NA.-.TF.NS.--.VKS........D.M.........R....E...VV.......L......SA------KGI.A.Y
Eimeria tenella mBiP DEEAIAKER.IL.Y..IRA-.N-G.AW..GW.-------.KYS.SQ.G.F..M......-.S...RD--.NQ..I........S.........K....D............L...ME.E.------GRT.A.Y
C. elegans: mBiP E.....K.L.V..Y.I.KA-SN-G.AW..AQ.-------.EYP.SGVG.F..M......-.S...TT--.N.P..........S.........Q.S......V........L......DA-----.D.IIA.Y
Dros.: mBiP D....KK..TNLSY...KA-SN-G.AW.SSTD------G.VYS.SQ.G.FI.M......-....NTP--.K............S.........Q........V........L...M..TE------DKI.A.Y
Trypan. cruzi mBiP E.SNI.H...N..Y.IGRS-SN-G.AW.QDAN------G.QYS.SQVG.F..E......-.NF..R.--.S.....C.....GP................I.VV.G.....L......TK------DSM.A.Y
T. cruzi: mBiP E.SNI.H...N..Y.IGRS-SN-G.AW.QDAN------G.QYS.SQVG.F..E......-.NF..R.--.S.....C.....GP................I.VV.G............TK------DSM.A.Y
Leishmania: mBiP E.EHI.K...N..Y.I.RA-GN-G.AW.QDGN------G.QYS.SQ.G.F..E......-.NF..H.--.S.............................I.VV.......L...M..TK------DSL.A.Y
Crithidia: mBiP E.EHI.T...N..Y.I.RA-AN-G.AW.QDGN------G.QYS.SQVG.F..E......-.NF..H.--.S.....C.......................I.VV.......L...M..TK------DS..A.Y
mouse: mBiP D.....K.T.N....I.RA-SN-G.AW..AH.-------.LYS.SQ.G.F..M......-.N...HT--AK...I........S.........Q.S......V........L......SE------D.V.A.Y
Homo sapiens mBiP D.....K...N....I.RA-SN-G.AW..AH.-------.LYS.SQ.GAF..M......-.N...HT--AKN..I........S.........Q.S......V........L......SE------D.V.A.Y
Rattus sp. mBiP D.....K.T.N....I.RA-SN-G.AW..AH.-------.LYS.SQ.G.F..M......-.N...HT--AK...I........S.........Q.S......V........L......SE------D.V.A.Y
Cricet. gris. mBiP D.....K...N....I.RA-SN-G.AW..AH.-------.LYS.SQ.G.F..M......-.N...HT--AK...I........S.........Q.S......V........L......SE------D.I.A.Y
pea: mBiP D.AQT.KEM.M..Y.I.RA-PN-G.AW..AN.-------QQYS.SQ.G.F....I....-......T--ISK.....................R....D.Q..........LS..MNNKG-------GL.A..
bean: mBiP D..QT.KEM.M....I.KA-PN-G.AW..AN.-------QQYS.SQ.G.F.........-......S--.SK..I..................R....D.Q..........LS..MNNKG-------GL.A..
potato: mBiP D..QT.KEM.M..Y.I.RG-SN-G.AW..AN.-------QQYS.TQ.G.FI........-......S--INK..I.............I....A....D.Q..........LS..MNSKG-------GLVA..
Lycopers. per. mBiP D..QT.KEM.M..Y.I.KG-SN-G.AW..AN.-------QQYS.SQ.G.FI........-......S--INK..I..................R....D.Q..........LS..MNS.E-------GLVA..
Spinacia oler. mBiP S..QT.KEM.M..Y.I.KA-PN-G.AW..AN.-------QQYS.SQVG.F.........-......T--.SK.....................K....D.Q..........LS..MTN.E-------GL.A..
Giardia: BiP D.....K.M.LL.Y..I.K-..R.FV.LSGTNLPKELQN.IMS.............TI.-.D...E.--I.K.........S.S..S......R....D.V.......SSS........TQETSGKA......
yeast: BiP ..RS..K....L..N...K-....AVE.SV..-----.K.V.......G.I.G...QI.-.D...T.--..H...............................V..............S.-----K.HQ.I.Y
K. lactis: BiP ...T..R....L.YT...K-GN..YVE.TV..-----.K.E.....V.G.I.G...QI.-.D.....--..H.....................A.....I...V..............TE-----D.HQ.I.Y
S. pombe: BiP DEKTMAK...SF..HI...-KNR.LVE.NVG.-----KK.K.........I.S...Q..-......P--..HS...........................I..V..............T.-----T..H.V.Y
Achlya a.: BIP ..KS..A.K.LL.YLLITK-..P.Y...IV..-----.KDF.S...V...L.....QI.-.N....E--.ND..........................T.Q..............I...A----S....V...
Phaeodact. tr. BiP S.KS..A.K.L..YEIIS.-KD..M.E.IQNE-----K.FK.A.K.V.....Q...L..-.TF...E--IKH..........................K.E..............M..TG-----..S.V...
P. falciparum: BiP D.Q..VK.RSLL.YEI..N-Q...N.K.QI.D-----KDT..A..Q......E....I.-QSF...P--.K............................IV....Q.....L..A....E------.TS...Y
C. elegans: BiP ..KT..A....W....IDK-SN..SVE.KVGS-----DN.Q.....V.....V....I.-.S....E--.K.............................V...................-----..R.....
C. briggsae: BiP ..KT..A....W....FDK-SN..SVE.KVGS-----DN.Q.....V.....V....I.-.S....E--.KH..........V.....L.Y....V....V...................-----..R.....
E. granul.: BiP: H.KD..G...RY....I.K-NN..YMK.QVGS-----.E.G.A...V.....S....I.-.....TE--..H.....................A....T.................E...-----T.......
E. multiloc.: BiP: D.KD..G...RY....I.K-NN..YMK.QVGS-----.E.G.A...V.....S....I.-.....TE--........................A....T.....................-----T.......
Aplysia: BiP D.KS..H...FY....T.A-NN..H..AATGE-----GDRS.A.........S..RDI.-.E.....--I..............................M..................E-----........
Drosophila: BiP S.TN..H...FF.....EK-NS..H.S.DTSQ-----GA.V.A.........G......-.......--..H.....................V....Q.M..................E-----....V...
chicken: BiP ...S..Q.I.YL.....EK-KA..H...DVG.----GQ....A................-.......--..H............................M.................RE-----........
hamster: BiP ...S..Q...FL.....EK-KT..Y...DIG.----GQ....A................-.......--..H............................M.................RE-----........
rat: BiP ...S..Q...FL.....EK-KT..Y...DIG.----GQ....A................-.......--..H............................M.................RE-----........
human: BiP ...S..Q...FL.....EK-KT..Y...DIG.----GQ....A................-.......--..H............................M.................RE-----........
maize: BiP: ...A..................G-----..R.....
tobacco: BiP (A) D.K...R.M.L..Y.I..K-....Y...KI.D----G.....S.......I........-.......--IKD.....................V......A..................G-----........
tobacco: BiP (B) D.K...R.K.L..YEI..K-....Y...KI.D----G...V.S.......I........-.......--IKD.....................V......A..................G-----........
barley: BiP E.K...R.MRL..Y.I..K-E...Y...KI.D----G...V.S...V...I.G......-.......--IND.....................V......A.................RG-----........
Giardia: hsc70 ......A.L..FRSRSSCGPTRT.Q...VY..-----...........S.......DI.-SD...N.--..E.I.......S..HGK..QN...........................ST---SKK.R...I.
Achlya ambisexualis: S..I.KK.AA.F...I.EGEGD..L...TF..-----.E.N.S.....S..S.R.....-.N...QE--IKQ...........Q...S.....A....D.K..........L.....SNA-GNDGEKT...I.
yeast: hsc7OA1 D.ES..K.M.TW....IDV-..N.V.E.QYLE-----.....S.Q............I.-..KI...--.EK..I..................A.S....................GAGK---SEK.RHV.I.
Kluyveromyces: hsc70 DEES....M.TW....IDS-N.A.L.E..YL.-----.....S.Q...S........I.-..KI...--.EK.....................A.....................VGAGN---SEK.RHV.I.
yeast: SSA1 ......A.M..F...LIDV-....Q....F..-----...N....Q..S...G......-.S...A.--.ND...........S...................................G-----K.EHV.I.
yeast: SSA2 ......G.M..F...LIDV-....Q....F..-----...N....Q..S...G......-.S...A.--.ND...........S...................................G-----K.EHV.I.
yeast: SSA3 D....TT.A..F....ISR-....VV...Y..-----...........S...S......-.N...TT--.ND...........S.............M.....................GR----A.H.V.I.
yeast: SSA4 D....TN.A..Y....IDK-G...VV...Y..-----.....T..E..S.I........-.NF..TE--.KD...........S...................................SQ----K.H.V.I.
Hansenula: hsp70 D.....N....F.....EK-G...H....F..-----...V.......S..........-.S.M.G.--..D..I........S.........L.........................EQG--K..Q...I.
Ustilago: D.A.....M..W..
Cladosporium: hsp70 Q.A...A.M..F....IEK-A...VT...F..-----...D.......S.I....R...-.S...GT--.N...I........S.........L.........................QE----....V.I.
Blastocladiella: hsp D.DV..A.M..WS.T...K-NS..LF...F..-----.........F.S.I........-.....T.--.NH...........S.........A.........................AEA---....V.I.
Bursaphel.: DE.T..A.M..W....I?GRS.R.KV...Y..-
Entamoeba: hsc70: S..AI.N.M..WS...ID.GHD..L.E..Y..-----.V.K..................-.SFV..E--.K...I.C......S.............M..M..................SD----R...V.I.
Trich. vagin. S..V..K.M..W.....RS-..DRPKIEVQFKG----...Q.F.........G......-.NM.SE.--I.D...........G.........V...................F..NE.SD----K.RHV.I.
T. brucei: hsp7OA4 S.SV....M..W.....TKG.D..V...QFR.-----.....N.....S...L....V.-.S....Q--.AK...........S..............E.....A.............A.EG---K.R.V.I.
T. brucei: hsc70 S.SV....M..W....EEGEK.GAVMR..HL.-----.GMLLQ..Q...R..AYL.SC.-.S....Q--.AK...........S..............E...................A.EG---K.R.V...
T. cruzi: hsp7OA8 S..V....M..W....ITKG.D..V...QFR.-----.....N...V.S...S....I.-.S....Q--.KK...........S..............E...................VEDG---K.R.V.I.
L. major: hsc70.4 D..DL...M.HW....IVK-....V.S..YQN-----Q....F......T..Q......-.....TT--.KD..I........S.........S.....................M.R.GDK---....V.I.
L. amazonensis: A ..SV....M..W....TTKG.D..V.S.QYR.-----.E......K..S...L......-......Q--.KK...........S...........S..E...................G.DG---K.R.V.I.
L. amazonensis: C ..LV....M..W....TTKG.D..V.S.QYR.-----.E......K..S...L......-......Q--.KK...........S...........S..E...................G.DG---K.R.V.I.
L. amazonensis: D..DL...M..W....TVK-....V.N..YQ.-----QS...F.........Q......-.....TT--.K............S.........S.....................M.R.AEK---....V.I.
Leishmania: hsp7OA3 ..SV....M..W....TTKG.D..V.S.QYR.-----.E.........S...L......-......Q--.KK...........S..........T...E.V.................G.DG---K.R.V.I.
Leishmania: pseudo D.\\\...M..W....IVK-....V.S..YQN-----Q....\.........Q......-.\...TT--.KD..I........S.........S.....................M.R.GDK---....V.I.
L. donovani: hsc70 ..SV....M..W....TTKG.D..M.A.QYR.-----.E.........S...L......-......Q--.KK...........S..............E.........R.-.......G.DG---K.R.V.I.
Theileria: D.RT..E.M..W....T.GPN...N.E.TFQ.-----.K...HA....S........I.-.SF...S--.KDV.I........S................M..................GG----....V.I.
Theileria parva D.RT..E.M.HW....T.GPN...N.E.TFQ.-----.K...HA....S........I.-..F...S--.KDV.I........S................M..................GG----....V.I.
P. cynmolgi: hsp70A2 TESS....M..W..T.KSGV.E..M.E.SYQ.-----.K.L.H.....S...Q....N.-..F...S--IK...I........S................M...............H..GK----......I.
P. falcip.: hsp70A TESS....M..W..T.KSGV.E.PM.E.TYQ.-----.K.L.H.....S...Q....N.-..F...S--IK...I........S................M...............H..GK----......I.
Eimeria: hsc70 D..A..A.M..W..T.KAGPG...L.E.NYQ.-----SK...H.........M....I.-..FI..E--.KE..I........S...................................GH----..M.V.I.
Euplotes eurystomus ...I..E...LW....ERGT.D..M.R..F..-----.S.L.QA.Q..S..........-......T--.KDD.I........S.........V.........................SS----A.....I.
Cryptosporidium par. D.QA....MT.W.....RGPKD..I.S.NYL.-----.K.E.HA......A.Q.....S-.....RQ--IK............S.........A......M..................GT----..R.V.T.
Babesia microti S..CI.N....W..T.AAGPNE..V.K.QFQ.-----...S.H.....S..........-.S....T--.S...I........S................M..................GT----S.R.V...
Chlamy.: hsc70: S..I......LW.SQ.APA-HDV.E.V.SYRT-----.E.V.KA....S...I......-Q.S..ADRE.KK...........S.........M....E.............S.......SGL--..R.V.I.
Bremia: hsp7O S..I..A....W...LTSG-WRQAQ.V.QF..-----.S...Q.....S...I..R.V.-..FI..E--.K...I........S.........A.........................G-----..R.V.I.
Achlya: hsc70 ...AT.A....W....TPGAGD..Q.T..F..-----.....Q.....S...I....V.-..FI.TA--.N...I........S.........A..............R..........G-----..R.V.I.
Pyrenomonas: hsp70 Q..A..E.V.PF....ICKDGD..AVE.KY..-----...I.A.....S...L....I.-.SF...E--.K...I........S.........A.T.......................TSGSK-S.R.V.I.
Ascophyllum nodosum G.ST....M..WS....CRDGD..H...NF..-----...V.A.........V..R...-...M..E--.KD...........S...................................GA----..R.V.I.
Guillardia th. hsc70 D.SSL.A.L..F.....PK-....H..A.Y..-----.N...S.....S.I.......S-......E--.KH..I........S.........L......................E..GT----..R...IY
carrot: hsp70: .H.S....M.LW.LQ.IPGPGE..M.V.NY..-----.S.Q.AA....S...I..L.I.-..F..HS--.ND...........S.......T.V......M......NC.Q........SSNP--P.Q.V.I.
petunia: hsp7O S..S......LW....IPGPGD..M.V.TY..-----.E.Q.AA....S........I.-.....TT--IK............S.........V......M..................ASSA--....V.I.
tomato: hsc70A S.AS..E.M.LW....IPGPGD..M.V.TY..-----.E.E.A.....S........I.-..F..ST--.K............S.........V.S....M..................ATSA--....V.I.
tomato: hsc70B S.AS....M.LW....IPGPGD..M.V.NY..-----.E.Q.SA....S...I....I.-..F..TT--.K.........S..S.........V.S....M..................ATSV--....V.I.
Arabidopsis: 2 S.AS....RQLW..TIISELQ
Arabidopsis: 1 S.SS....M.LW...IQAGPAD..M.Y..Y..-----.E.E.AA....S...I..R.I.-.....VT--IK............S.........V......M..................ATRV--....V.I.
spinach: hsc70 S.AS..A.M..R.....SGPGE..M.G.NY..-----.E.Q.AA....S........I.-.....ST--.K............S.........V.S....M..................ATSV--....V.I.
soybean: hsp70 S.SS..N.M.LW....GGSPCD..M.V.NY..-----.E.K.SA....S...V..R.V.-EAF..HA--.K............S.........A.S.......................ASRK--..Q.V.I.
potato: hsp70DK S..T
tobacco: hsp70 ..S....M..W.....PGPGD..M.V.QY..-----.E.Q.A.....S........V.-..F..QT--.K............S.........A.S....M..................ASRS--....V.I.
maize: hsp7OA1 SS.A...SM.LW.SRHLGL-GD..M.VFNY..-----.E.Q.AA....S...I....I.-.....ST--IK............S.........V......M..................ATSS--....V.I.
lily: hsp70 S..S..A.M.LW....ISGTRD..M.V.QY..-----.E.Q.AV..V.S...V..R.I.-V....RS--IN............S.........A.........................ATSP--S...V.I.
sea urchin: hsp70 ..SSI.A.M..W..R.I.K-....ML.A.YM.-----....LS...V.S..........-.......--..S..I..................V...I..........R..L.......LT----...HV.I.
sea urchin: hsp70-4 ..SSI.A.M..W..R.I.K-....ML.A.YM.-----....LS...V.S..........-.......--..S..I..................V...I..........R..L.......LT----...HV.I.
Trich. britovi mBiP? D.AA....M..W...II..-GS..K....Y..-----.S.S...........V......-......T--.KD..I........S...........S.....................R.GG----..R.V.I.
B. malayi: D.GS....M..W......AGG...KV...Y..-----...........S...V......-..FL.HA--.KD..I........S.......S.A.........................GH----..R.V.I.
C. elegans: hsp7OA D..A....M..W....ISAEGA..KV...Y..-----.N.I.......S...L...K..-..F.EPT--.KD......T....S.........A.........................GH----..R.V.I.
Dros.: hsc70-4 D.AA....M..W..E..SA-....K.E.TY.D-----.K...F.....S..........-......T--.....I........S...................................AV----..R.V.I.
D.sim.: hsc7O-1 D.AT....M..W..EAFAE-N...R.R
Dros.: hsc70-1 D.AT....M..W..E.FAE-N...R.R..Y..-----.R.S.Y...V.S......R...-.....GT--..D...........S.........A........................QGT----S.R.V.I.
Dros.: hsc7O-2 D.KKI.E.L.LW....I.E-K...K.E
mosquito: hsp70-1 D..KI.A.M..W..T....-C...K.R..F..-----.R...A.....S..........-.....QS--.K...I........S.........A.................L......NLK----..R.V.I.
mosquito: hsp70-2 D..KI.A.M..W..T....-G...K.R..F..-----.R...A.....S..........-.....QS--.K...I........S.........A......M..........L.......LK----..R.V.I.
mosquito: hsp70-3 D..KI.A.M..W..T....-G...K.R..F..-----.R...A.....S..........-.....QS--.K...I........S.........A......M..........L......NLK----..R.V.I.
Dros.: hsp7OA2 D..KIAE.M..W.....S.-G...K.G..Y..-----.S.R.A.....S..........A.....ES--I.D..I........S.........H.................L......NLK----..R.V.I.
Dros.: hsp7OA4 D..KIAE.M..W.....S.-G...K.G..Y..-----.S.R.A.....S..........-.....ES--I.D..I........S.........H.................L......NLK----..R.V.I.
Schistosoma: P..S....M..W..E.TQV-G..LK.C..Y..-----.K.M.S.....S........V.-.S...RT--.SD..I........S.........A.........................VG----..R.V.I.
Hydra m.: hsp70 ...S.T..R..W..N.ID.-GSR.K....F..-----...S.Y.....S...L..E.I.-D......--..DV.I........S.........V.........................VG----T...V.I.
Hydra o.: hsp70 ..L....I.-D......--..DV.I........S.........V.............N...........VE----K...V.I.
sea urchin: S.TN.KA.M.EW..T.IEE-G.R.K.KI.F..-----.S...YA....S...L......-......ES-..D...........S......EC.V.S.M.I.............T.....GG----A.R.V.I.
trout: 7O.14 D.GV....M..W..E.I..-STR.K....Y..-----...S.Y.....S...V....I.-......T--.N............S...........S................RT.....VG----A.R.V.I.
salamander: hsp70 ..TV..A.M..W.....S.-EW..KV...Y..-----DN...F.D.V.S...I....I.-.....HL--.S...I...S.LTAS.........VL......S.V..........D...AGR----..L.V.I.
rat: hsc7OA D.AV....M..W..M....-A.R.KV...Y..-----...S.Y...V.S........I.-......T--..............S...................................VG----A.R.V.I.
mouse: hsc7OA D.AV....M..W..M....-A.R.KV...Y..-----...S.Y...V.S........I.-......T--..............S...................................VG----A.R.V.I.
mouse: hsc7OA* G----A.R.V.I.
hamster: hsc7OA D.AV....M..W..M....-A.R.KV...Y..-----.A.S.Y...V.S........I.-......T--..............S...................................VG----A.R.V.I.
cow: hsc70 D.AV....M..W..M....-A.R.KV...Y..-----...S.Y...V.S........I.-......T--..............S...................................VG----A.R.V.I.
human: hsc7OA D.AV....M..W..M....-A.R.KV...Y..-----...S.Y...V.S........I.-......T--..............S...................................VG----A.R.V.I.
mouse: pseudo2 D.AL....M..WFVM.L..-A.R.KV...Y..-----K..S./L..V.S....... .....Q . ...........
human: pseudo1 D.AG...AM..W..M....-A.R.KV...Y..-----...S.Y..DV.S........I.-......T--............../.C..N.........................S....VR----A.R.M.I.
chicken: D..T....M..W..R...E-G...KV...Y..-----.M...F.....S........I.-.......--.ET..I........S...........T....M..................GTRA--....V.I.
mouse: hsc7OB E.AT....M..W..R..SE-G...KV...Y..-----.M...F.....S........I.-.....G.--.QS..I........S...........T.......................GCAG--....V.I.
human: hsc70B E.AT....M..W..R..SE-G...KV...Y..-----.....F.....S........I.-.....G.--.HS..I........S...........T.......................GCAG--....V.I.
cow: hsp70 E.AT....M..W..R..SE-G...KV...Y..-----.I...F.....S..........-.....G.--.QS..I........S...........T.......................GCAG--....V.I.
trout: 7O.7 .-.....Q.--.S...I........S.........V................S....M..GMS----R.R.V.I.
Xenopus: hsp7OA1 ...V..C.L..W..Q..S.-E...KVK..Y..-----.E.S.F.....S..........-.....HP--.....I........S.........VL....I..................GAR----..Q.V.I.
Xenopus: hsp7OA2 ...V..C.L..W..Q..S.-E...KVK..Y..-----.E.S.F
mouse: hsc70C ...V....M.LW..Q.I.E-A...KVM.SY..-----.K.A.Y.....S..........-..F..HN--.....I........S.........V........................GSH----..RHV.I.
mouse: pseudo? ...V...NMKLWPFQ/I.E-A...KVM.SY..-----.K.A.Y.....S..........-.V//.H/--..T/.I........S.........V...............V.W.R-...GSH----A.RHV.I.
human: hsp7OB A.TT....M..W..R..SE-G...KVP.SYR.-----.D...Y.....S..........-.....QP--.KH..I........S.........A.......................RRGA----..R.V.I.
pig: hsp70com G..V..G.M..W..R.I..-GD..KV..SY..-----...G.Y.....S........I.-.G...HP--.S...I........S.........V.......................RTGK----..R.V.I.
mouse: hsp7OA1 G.AV....M..W..Q....-GD..KV..NY..-----.SRS.F.....S........I.-.....HP--.....I........S.........V.......................RTGK----..R.V.I.
mouse: hsp70A2 G.AV....M..W..Q....-GD..KV..NY..-----.SRS.F.....S........I.-.....HP--.....I........S.........V.......................RTGK----..R.V.I.
human: hsp7OA1 G..V....M..W..Q.I..-GD..KV..SY..-----...A.Y.....S........I.-.....YP--.....I........S.........V.......................RTGK----..R.V.I.
human: hsp7OA2 ..-.D..K...SY?.-----...A.Y.....S........I.-.....YP--.....I........S.........V.......................RTGK----..R.V.I.
CONSENSUS NDPEVQSDIKHVPFKVVND-DGKPDIQVEXKG-----ETKTFTPEEISAMVLTKMKETA-EAYLGKK--VTNAVVTVPAYFNDAQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKDX----GEKNILVF
*NO1
*NO2 200 21O 225 24O 255 27O 285 3OO
*NO3 | | | | | | | |
*2nd CONSENSUS DLGGGTFDVSILTIDDG----VFEVKATNGDTHLGGEDFDQRIVNHLVEEFKKKN-GIDLS-----------KDKRALQRLREAAEKAKRELSSSTQTXIE---IDALFEG------KDFXXTLTRAKFE-ELN
E. coli: hsc70 ........I...RLSR.----....L..G..SA...D...HLLADYIRF....QA-..PDR-----------S.N.VQRE.LD..IA..IA..DADSVTVN---------------VAGWQGEIS.EQ.N-..I
H. influenzae: hsc70 ........I...RLSK.----I...L..G...A...D...HL.ADWVI.QT.L.P-QTANQ----------------QRE.ITL.NQ..IT.TNEKSAV.S---------------WQ..SVEIS.EQ.N-..I
Buchnera aph. hsc70 ........I.V.KLNK.----I...LG.A.HAN...D...TLLSKYIYKKLNLS.-QC.NF------------------FQSLLLK...EIKIKL.KYEKV---EVNF.---------NWKGIIF.DE.N-LII
B. megaterium: ............ELG..----....R..A..NR...D....V.IDY..A....E.-.V...-----------...M.....KD......KD..GV.S.Q.SLPF.T.GEA.-----PLHLEVS.S....D-..S
B.subtilis: dnaK ............E.G..----....RS.A..NR...D....V.ID...S....E.-.V...-----------...M.....KD......KD..GV.S.Q.SLPF.T.GEA.-----PLHLEVS.S....D-..S
Bacillus stearo. ............ELG..----.......A..N....D....V.IDY..DQ..QEH-.....-----------...M.....KD......K...GV...Q.SLPF.S.NEN.-----PLHLET........-..F
Staph. aureus: ............ELG..----....LS.A..NK...D....V.IDY..A....E.-.V...-----------Q..M.....KD......KD..GVS..Q.SLPF.S.GEN.-----PLHLEVN...S...-..S
Streptococcus pyog. ............ELG..----..D.L..A..NK...D....K.IDF..A....E.-.....-----------Q..M.....KD......KD..GV...Q.SLPF.T.GSA.-----PLHLEMS.S....D-D.T
Streptococcus mutans ............ELG..----..D.L..A..NK...DN...KVIDW.......E.-.....-----------T..M.....KD......KD..GV.S.Q.SLPF.T.GEA.-----PLHLETS.S....D-D.T
Streptococcus agal. ............ELG..----..D.L..A..NK...D....K.IDF.......E.-.....-----------Q..M.....KD......KD..GV...Q.SLPF.T.GSA.-----PLHLEMS.S....D-D.T
Streptococcus pneu. ............ELG..----..D.LS.A..NK...D....K.ID...A....E.-.....-----------T..M.M...KD......KD..GV.S.Q.SLPF.T.GEA.-----PLHLEM.......D-D.T
L. lactis: dnaK ............ELG..----..D.L..A.NNK...D....K.IDWM.A....E.-....G-----------Q..M.....KD......KD..GV.T.Q.SLPF.T.GAA.-----PLHLEMA......D-..T
Erysipelothrix: dnaK ............ELA..----T...LS.A..NK...D...NIV.DY..DI...E.-.....-----------S..M.M...K.......KD...TVNAS.SLPF.S.GEN.-----PLHLET..S.....-.MT
Mycoplasma: dnaK ............A.GG.----S.D.I..S.NNK...DN..EE.IKW.LGKI.AEY-N....-----------.E
Mycoplasma pneum. ..........L.D.AE.----T...L..A..NR...D.W.NK.IEFILAHIAQEHN.LN..-----------N..M.M...K....R..I...AQLEAI.SLPFLTVTEK.-----PVNVELK.......-.IT
Mycoplasma gen. ..........L.D.AE.----T...L..A..NR...D.W.NK.IEYISAYIA.EHQ.LN..-----------...M.M...K....R..I...AQLE.I.SLPFLTVTQK.-----PVNVELK.......-..T
Mycobacterium tuber. ..........L.E.GE.----.V..R..S..N....D.W...V.DW..DK..GTS-....T-----------...M.M...........I.....QS.S.NLPY.TVDADK----NPLFLDEQ....E.Q-RIT
M. leprae: dnaK ..........L.E.GE.----.V..R..S..N....D.W.D....W..DK..GTS-....T-----------...M.M...........I.....QS.SVNLPY.TVDSDK----NPLFLDEQ.I..E.Q-RIT
M.paratuberculosis: ............E.GE.----.V..R..S..NQ...D.W.D....W..DK..GTS-....T-----------...M.M...........I.....QS.S.NLPY.TVDADK----NPLFLDEQ....E.Q-RIT
Streptomyces: dnaK ..........L.E.G..----.V........NN...D.W...V.DY..KQ.QSGH-.V..A-----------...M.............I......E.TINLPY.T.SA..-----PLHLDEK....Q.Q-Q.T
S. griseus: dnaK ..........L.E.G..----.V........N....D.W...V.DY..KQ.ANGH-.V...-----------...M.............I......E.T.NLPY.T.SA..-----PLHLDEK...SQ.Q-Q.T
Clostridium: dnaK ............DLG..----....VS....AR...D......IDYIA.D..GE.-....R-----------Q..M.....K.GRQ...I........L.NLPF.T.DAT.-----P.HIDM.......N-..T
Clostridium aceto.: ..........V.ELG..----.....S...N.....D...EK.MDYIA.....D.-....R-----------N..M.............I........N.NLPF.T.DAT.-----P.HIDMN......N-..T
Methanosarcina: ............ELGG.----.....S.S.......D.....VIDY.LA....SE-.....-----------...AV....KD......I...GVAN.N.NLPFLTVGTD.----EP.HMDID....Q.Q-KMT
Halobacterium: ............DLGG.----.Y..V.....ND...D.W.HA.IDY.AD..EAEH-....R-----------D.RQ.....T....E..I....RKE.R.NLPF.ATTDD.-----PLDLEQKI......-S.T
Halobact. cut.: ............DLGG.----.Y..A.....ND...D.W.HA.ID..ADN.ENEH-....R-----------E.RQ.....T....E..I....RKE.TVNLPFVT.TDS.-----PVHLEQDI...T..-SIT
Thermoplasma a.: ......L..T.MDFG.A----..Q.LS.S...R...T.M.EA...YIADD.Q..E-....R-----------..RS.YI...D......I...TTLS.D.DLPY.TVTNS.-----P.HIKM......L.-..Y
Synechocystis: dnaK1 ..........L.QLGN.----....LS.S.NN....D...NCV.QWMA.S..Q.E-N....-----------T..M.I...........I....MLN.T.NLPF.T.DES.-----P.HLEME.A.SQ..-..T
Synechococcus: dnaK1 ....S.L.....QLG.S----.......A.NN....D...AV..DW.ADN.L.AE-S...R-----------Q..M.I......S.Q..ID..TLPT.T.NLPF.ATATVD-GAPEP.HIEVE.Q.EQ..-V.A
Therm. aquaticus .........T..E.GE.----.......S.......S.M.HA...W.A.....EH-.V..K-----------A.RQ.....I.......I....TLE.T.SLPF.ALDPAS---KTPLHLEKK.......-..I
Deinococcus prot.: .........T..ELG..----.....S.S...S...A.......DW.A...N.EH-NF..R-----------...Q.....I.......I...NASE.S.SLPF.TFDP.T---RTPLHLER..S.....-..T
Thermomicrobium ros: ......Y.I...D.SE.----..Q.L....Y..TPLVH...A.IDLALH...RET-....R------------QD.IV...K.......I....VQ..E.NLPF.T.DAT.-----P.HLTVK....RLQA..V
Synechocystis: dnaK2 ............EVGE.----....L..S.......D...KK..DF.AG..Q.AE-....R-----------...Q.....T.......I...GVS..E.NLPF.T.TQD.-----P.HLDT..S.....-.IC
Synechococcus: dnaK2 N.........V.EVG..----....L..S.......D...KK..DF.AG..Q.NE-....R-----------...Q.....T.......I....A...E.NLPF.T.TQD.-----P.HLDL........-..A
Synechocystis: dnaK3 ..........V.EVG..----I......S...Q...N...R...DW.A.K.LEAE-KV..R-----------Q.RQ.....T.......I...GVGT.E.NLPF.T.TED.-----P.HLETQ.S.SE..-D.C
Synechococcus: dnaK3 ..........V.KVGN.----.......S...Q...N...R...DW.A.Q.LEAE-....R-----------R.RQ.....I.......I...GVSV.D.NLPF.T.TEDE-----P.HLETR...SE..-A.C
Anabaena variabilis ............EVG..----.......S...Q...N...KK..DW.A.Q.LETE-.V..R-----------R.RQ.....M.......I...AVSI.D.NLPF.T.TED.-----P.HLETR...SQ..-G.C
Aphanothece halo. ......L.....QLG..----.......A.NN....D...NV..EWMLS..QAQE-..N.N-----------E..M.M........R..I...TRPT.S.NLPF.T.FSK.GGEVGP.HLKLN.S....N-..S
Porphyra: cBiP: ............EVG..----....LS.S.......D....Q..EW.IKD..QSE-....G-----------..RQ.....T..S....I...NL...E.NLPF.T.TQD.-----P.HLEK.V......-..C
Chlamy. rein. cBiP ..........V.EVG..----....LS.S.......D...K...DF.ADD...SE-....R-----------..RQ.....T.......I...GMA..S.NLPF.T.TAD.-----P.HIDTQ.......-.MC
pavlova: cBiP ............EVG..----....LS.S...R...D...EK..KW.LN..E.EE-KFS.K-----------G.SQ.....T.......I....LS..E.NLPF.T.NEN.-----A.HIEK...GE...-S.C
Odontella sin. cBiP ............EVG..----I...L..A...N...D...KVL.RW..K..EDQE-....T-----------Q.IQ.....T.......M...TVEK.T.HLPF.T.DKT.-----P.HIEKE...KT..-T.C
Cryptomonas: cBiP: ..........V.EVG..----....LS.S.......D...DK..QW.LK..ETEH-S.N.K-----------S.WQ.....T..S....I...NLS..E.NLPFLT.TET.-----P.HLERSI......-..C
Spinach: cBiP ..........V.EVG..----....LS.S.......D...K...DW.ASS..RDE-....L-----------...Q.....T.T.....M....L..AN.SLPF.T.TAD.-----P.HIET........-..W
Pisum sativum cBiP ..........V.EVG..----....LS.S.......D...K.V.DW.AGD..RDE-....L-----------...Q.....T.T.....M....LS..N.SLPF.T.TAD.-----P.HIET.L......-..C
Cucumis sativ. cBiP ..........V.EVG..----....LS.S.......D...K...DW.AAN..RDE-....L-----------...Q.....T.T.....M....L..AN.SLPF.T.TAD.-----P.HIET.I......-..C
Cucumis sativ. mBiP ..........V.EVG..----....LS.S.......D...K...DW.AAN..RDE-....L-----------...Q.....T.T.....M....L..AN.SLPF.T.TAD.-----PKHIET.I......-..C
Citrullus lan. cBiP ..........V.EVG..----....LS.S.......D...K...DW.AAN..RDE-....L-----------...Q.....T.T.....M....L..AN.SLPF.T.TAD.-----P.HIET........-..C
Aquifex aeolicus .F..........EGGE.----.I...V.A.......ANI.E..MDW.I.....ET-....R-----------..RT.....K..S.Q..K...FKME.E.NLPF.TIDPNT---NQPLHLQKK....RL.-.MI
C. pneumoniae: ........I...E.G..----....LS.....L...D...EV.IKWMI.....QE-.....-----------..NM.....KD......I...GVSS.E.NQPF.TMDAQ.-----P.HLAL.....Q..-K.A
Chlamydia: dnaK ........I...E.G..----....LS.........D...---------....QE-.....-----------..NM.....KD......I...GVSS.E.NQPF.TIDAN.-----P.HLAL.....Q..-H.A
Chlamydia pneumoniae ........I...E.G..----....LS.....L...D...EV.IKWMI.....QE-.....-----------..NM.....KD......I...GVSS.E.NQPF.TMDAQ.-----P.HLAL.....Q..-K.A
Borrelia: dnaK ........I...ELG..----.....S.........DN..DE.IK..IS....ES-A....-----------N..M.....K.......I...GAQEAS.NLPF.T.DAN.-----P.HLQY........-QMV
Leptospira interrog. ........I...ELA..----.....S.........D...MA.MEWMIS...NQT-...I.-----------A..NTV...K.......I...GTMS.Q.NLPF.T.DAS.-----P.HLDM.......D-Q.T
Helicobacter pylori: .........TV.ETG.N----.V..L..G..AF...D...N.VIDF.AS...SET-..EIK-----------N.VM.....K....N..K....AME.E.NLPF.T.DAT.-----P.HLVKK.......-S.T
Myxococcus xanthus ........I...ELNA.----.....S.....F.........LIDY.AKR.AES.N.L..R-----------..RM.....K....R..H....APE.EVNLPF.T.DAS.-----P.HLTE.VD..T..-A.V
Caulobacter: dnaK ............E.G..----.....S.....F.......L...DY.AD....EQ-.V..R-----------...L.......-.....K....TA.YEVNLPF.SMNAS.-----PLHLNIK.S...L.-A.V
Rhizobium meliloti: ........I.V.E.G..----.....S.....F.......M.L.EY.AS....EQ-....K-----------N..L.....K.......I.....Q..E.NLPF.T.DAS.-----P.HLTMK.S.....-S.V
Rhodoba. capsulatus: ........I.V.E....----L....S.....F.......M....Y.AD....EH-.V..T-----------...M.....K.......I....AS..E.NQPF.SMNAAT---GVPLHMVMK.....L.-S.V
Agrobacter. tum.: ........I.V.E.G..----.....S.....F.......M.L.EY.AG....DQ-....K-----------N..L.....K.......I.....Q..E.NLPF.T.DAS.-----P.HLTPK.......-S.V
Brucella: dnaK ..........VLE.G..----.....S.....F.......I.L.EY..A....ES-....K-----------N..L.....KD......T.....Q.TE.NLPF.T.DQT.-----P.HLAIK.S..NV.-S.V
Rhodopseudomonas sp. ............E.G..----.....S.....F.......M.L..Y.AD..Q.EQ-....R-----------...L.....K.......I....T...E.NLPF.T.DQS.-----P.HLTMK.......-A.V
Rhizobium legum.: ........I...E.G..----.....S.....F.......M.L.EY..A...RD.-....K-----------N..L.....K.......I.....Q..E.NLPF.T.DAS.-----P.HLTLK.....L.-S.V
Bradyrhizobium japo. ........I...E.G..----.....S.....F.......M.L.GY.AD..Q.EQ-..N.R-----------N..L.....K.......I....T...E.NLPF.T.DQT.-----P.HLTMK.......-A.V
Pseudomonas cepacia: ...........IE.A.VDGEMQ...LS.....F.......Q..IDYIIG....EQ-.V..S-----------..VL.....K.......I.....Q..E.NLPY.T.DAS.-----P.HLNLKI....L.-A.V
Ralstonia eutropha ........I..IE.A.VDGEKQ...LS.....F..........IDYIIG...REQ-.V...-----------..VL....CK.......I.....Q..E.NLPY.T.DAS.-----P.HLNLKI....L.-A.V
Nitrosomonas europ. ........I..IE.AEVEGEHQ...L......F.......S.VIEY..D..R.ES-....K-----------..ML.....KD......I.....Q..EVNLPY.T.DAS.-----P.HLAVKI....L.-S.V
Franc. tularensis: ........I..IE.S.VDGDNQI..LS.....F.......LALM.Y.ID....EQ-....H-----------N..L....V........V...PAQ..DVNLPY.T.DAT.-----P.HLNIKV......-S.V
Legionella pneumo. ........I..IE.AEVDGEHQ...L......F.......LALIEY.AS....DT-....H-----------N.PL.....K.......I....AQ..DVNLPY.T.DAS.-----P.HLNIK.....L.-S.V
Haemoph. actinomyc. ........L..IE..EVGGEKT...L..............N..I.Y..D....EQ-....R-----------N.PL.M...K.......I....AQ..DVNLPY.T.DAT.-----P.HLNIK.....L.-S.V
Haemoph. influenzae: ........I..IE..NFDGEQT...L..G.N.........N.VIDYIID....EQ-N...R-----------N.AM....VK.......I....AQS.EVNLPY.T.DAT.-----P.HLALNI....L.-A.V
Haemoph. ducreyi: ........I..IE..NFDGEQT...R..............N.VI.Y.....Q.EQ-.V..R-----------N.PM.M..VK.......I....AQE.EVNLPY.T.DAT.-----P.HLNIKV....L.-A.V
Pasteurella haem. ........I..IE..NFDGEQT...R..............N.VI.Y.......Q--.V..R-----------N.PM.M..VK.......I....AQE.EVNLPY.T.DAT.-----P.HLNIKV....L.-S.V
Vibrio cholerae ........I..IE..EVEGEKT...LS.............N.MI....D....DQ-....R-----------N.PL.M...K.......I....AQ..DVNLPY.T.DAT.-----..HMNIKV....L.-A.V
Buchnera sp. ........I..IE..EVDKEKT...L..............S.LI.Y..T....EQ-....R-----------N.PLSM...K.S.....I....AQ.TDVNLPY.T.DSN.-----P.HLNIKV....L.-S.V
Salmonella typh.: ........I..IE..EVDGEKT...L..............T.LI.Y..D....DQ-....R-----------N.PL.M...K.......I....AQ..DVNLPY.T.DAT.-----P.HMNIKV....L.-S.V
E. coli: dnaK ........I..IE..EVDGEKT...L..............S.LI.Y.......DQ-....R-----------N.PL.M...K.......I....AQ..DVNLPY.T.DAT.-----P.HMNIKV....L.-S.V
E. coli B: dnaK ........I..IE..EVDGEKT...L..............S.LI.Y.......DQ-....R-----------N.PL.M...K.......I....AQ..DVNLPY.T.DAT.-----P.HMNIKV....L.-S.V
S. pombe: mBiP ........I...ELNN.----....RS.............VAL.R.I..T...NE-.L...-----------..RL.V..I........C....LSK.D.SLPF.T.DAT.-----P.HINMEIS..Q..-K.V
yeast: mBiP ........I...D..N.----.....S.............IYLLREI.SR..TET-....E-----------N.RM.I..I........I....TVS.E.HLPF.T.DAS.-----P.HINMKFS..Q..-T.T
yeast: Yel030p ........I...D..N.----I....S.............IYLLQEIISH...ET-.....-----------N.RM.V..I........I....TLS.E.NLPF.T.DAA.-----P.HIRMPFS.VQL.-NIT
Trich. vagin. hBiP ............E.SKDG---..........TF.......SALMK.VIQD.QA..-Q....-----------..PL....I...V....C....MMT.E.NLPY.TVTGA.-----PKHLQMPI...N..-KIT
Trich. vagin. mBiP ........F...E.SKDG---...........F.......AALMKYVIQD.QA..-Q...A-----------..PL....I...V....C....MMT.E.NLPY.TVTGA.-----PKHLQMPI...T..-KIT
Nosema locust. mBiP ........I...E.K..----I....S...N.......I.AE..DYVI.KAGLRH-KAGNM------------SAGT.K.I.RA..A..I...QADS.R.*TEFGKQDAAD---KYSVEVDVV...NEL.-DIA
Vairimorpha n. mBiP ........I..LEL.N.----I.H..S...N.F.....L.NKL.DYIN.K..S.T-....L-----------.NEN.YT.IK.Q...I.....TKL.SE.NIPY.YNDKKS-----NYHLKE.I..DE..-KIS
Eimeria tenella mBiP ............E.LG.----.........N.S........KVLQF..N.....E-.....-----------..RL..........T..I....KLS.E.NLPF.T.DQS.-----P.HLQVS.S..HL.-..V
C. elegans: mBiP ............E.QK.----.....S.....F.......HAL.H...G....EQ-.V..T-----------..PQ.M...........C....T...D.NLPY.TMDQS.-----P.HLNLK.......-QIV
Dros.: mBiP ........I...E.QK.----.....S.....L.......NH...F..V....DS-...IR-----------..NI.M...K.......C.....Q..D.NLPYLTMDAA.-----PQHMNLK...S.L.-S.V
Trypan. cruzi mBiP ........I.V.E.AG.----...................LCLSDYILT....ST-.....-----------NERM....I........C...TTME.EVNLPF.T.NQD.-----AQHVQMTVS.S...-S.A
T. cruzi: mBiP ........I.V.E.AG.----...................LCLSDYILT....ST-.....-----------NERM....I........C...TTME.EVNLPF.T.NQD.-----AQHVQM.VS.S...-S.A
Leishmania: mBiP ........I.V.E.AG.----.......
Crithidia: mBiP ..........V.E.AG.----...................LTLSDYIL...R.TS-.....-----------.ERM....V........C....AME.E.NLPF.T.NAD.-----AQHIQMHIS.S...-GIT
mouse: mBiP ........I...E.QK.----.....S.....F........ALLR.I.K...RET-.V..T-----------..NM....V........C.....V..D.NLPYLTMDAS.-----P.HLNMK....Q..-GIV
Homo sapiens mBiP ........I...E.QK.----.....S.....F........ALLR.I.K...RET-.V..T-----------..NM....V........C.....V..D.NLPYLTMDSS.-----P.HLNMK....Q..-GIV
Rattus sp. mBiP ........I...E.QK.----.....S.....F........ALLR.I.K...RET-.V..T-----------..NM....V........C.....V..D.NLPYLTMDAS.-----P.HLNMK....Q..-GIV
Cricet. gris. mBiP ........I...E.QK.----.....S.....F........ALLR.I.K...RET-.V..T-----------..NM....V........C.....V..D.NLPYLTMDAS.-----PKHLNMK....Q..-GIV
pea: mBiP ............E.SN.----...........F.......NALLDF..S...RTE-S...A-----------...L.............I....TS..E.NLPF.S.DAS.-----A.HLNI....S...-A.V
bean: mBiP ............E.SN.----...........F.......NALLDF..N...RTE-S....-----------..RL.............I....TS..E.NLPF.T.DAS.-----A.HLNI....S...-A.V
potato: mBiP ............E.SN.----...........F.......NALLEF..S...RTE-.....-----------...L.............I....TS..D.NLPF.T.DAS.-----A.HLNI....S...-T.V
Lycopers. per. mBiP ............E.SN.----...........F.......NALLEF..S...RTE-.....-----------...L.............I....TS..D.NLPF.T.DAS.-----AKHLNI....S...-T.V
Spinacia oler. mBiP ............E.SN.----...........F.......NTLLEF..S....TE-.....-----------S.RL.............I....TS..D.SLPF.S.DSS.-----AKHLNI..S.S...-S.V
Giardia: BiP .C....H.....SV.S.----....L..A.N.........R.LLD.FIAI.....-N....ITNTGDKAKDMAV.K.IS...REI.AG..Q..TASSVQ.V---V.S.ID.------I..SELV......-...
yeast: BiP ..........L.S.EN.----....Q..S...........YK..RQ.IKA....H-...V.-----------DNNK..AK.KRE......A...QMS.R..---..SFVD.------I.LSE........-...
K. lactis: BiP ..........L.S.EN.----....Q..A...........YKL.R.FAQLFQ..H-DL.VT-----------.NDK.MAK.KRE......S...Q.S.R..---..SF.N.------I..SE........-...
S. pombe: BiP ..........L.S..N.----....L..S.............VI.Y.ARTYNR..-NV.VT-----------..LK.MGK.KREV...NGT...QKSVR..---.ESF.N.------Q..SE..S.....-.IK
Achlya a.: BIP .........TL....G.----....LS...............VMQFFINKWQ.QK-K..I.-----------S......K..RE..RV..A..TQP.ARL.---.ES.LS.------E..TES.V..R.C-KV.
Phaeodact. tr. BiP .........TL....N.----....L................VMQYFIKMM...S-DV.I.-----------.......K..KEV.RV..A...QQ.ARL.---.ED.A..------F.LSE.....R..-...
Plasmodium: BiP .........VMDYFIKM.....-N...R-----------T....I.K..KEV.I...N..VVHS.P..---.EDIV..------HN.SE........-...
P. falciparum: BiP ............V..N.----....Y..A.N...........VMDYFIKM.....-N...R-----------T....I.K..KEV.I...N..VVHS.Q..---.EDIV..------HN.SE........-...
C. elegans: BiP ..........M....N.----....L................VMEYFIKLY...S-.K..R-----------.....V.K..REV.....A..TQH..KV.---.ES..D.------E..SE........-...
C. briggsae: BiP ..........M....N.----....L................VMEYFIKLY...S-.K..R-----------.....V.K..REV.....A..TQH..KV.---.ES..D.------E..SE........-...
E. granul.: BiP: ..........L....N.----....V..S.............LIDYF.KLY...E-.K.IT-----------..D..V.K..REV.....T..TEHS.M..---..N....------...SEP....R..-...
E. multiloc.: BiP: ..........L....N.----....V..S.............LIDYF.KLY...E-.K.IT-----------..D..V.K..REV.....T..TEHS.M..---..N....------...SEP....R..-...
Aplysia: BiP: ..........L....N.----....VS...............VME.FIKLY...K-.K.IR-----------..N..V.K..REV.....A...AH.VRL.---.ESF.D.------E..SES.......-...
Drosophila: BiP ..........L....N.----....V................VMD.FIKLY...K-.K.IR-----------..N..V.K..REV.....A..G.H.VR..---.ESF...------D..SE........-...
chicken: BiP ..........L....N.----....V................VME.FIKLY...T-.K.VR-----------..N..V.K..REV.....A...QH.AR..---.ESF...------E..SE........-...
hamster: BiP ..........L....N.----....V................VME.FIKLY...T-.K.VR-----------..N..V.K..REV.....A...QH.AR..---.ESF...------E..SE........-...
rat: BiP ..........L....N.----....V................VME.FIKLY...T-.K.VR-----------..N..V.K..REV.....A...QH.AR..---.ESF...------E..SE........-...
human: BiP ..........L....N.----....V................VME.FIKLY...T-.K.VR-----------..N..V.K..REV....-A...QH.AR..---.ESFY..------E..SE........-...
maize: BiP: ...............N.----....L.................MEYFIKLI...Y-SK.I.-----------..N...GK..RE..R...A..NQH.VRV.---.ES..D.------T..SEP....R..-...
tobacco: BiP (A) ...............N.----....LS................MEYFIKLI...H-.K.I.-----------..N...GK..RE..R...A...QH.VRV.---.ES..D.------V..SEP....R..-...
tobacco: BiP (B) ...............N.----....L.................MEYFIKLI...H-.K.I.-----------..N...GK..RE..R...A...QH.VRV.---.ES..D.------V..SEP....R..-...
barley: BiP ...............N.----....L..............H..MDYFIKLI...H-.K.IS-----------..N...GK..RE..R...A..NQH.VRV.---.ES..D.------T..SEP....R..-...
Giardia: hsc70 ..........L..V.PS--SG.......A...........S.V..YFIA.....H-.K.I.-----------GSN..MR...T.C.E...T....Q.AS..---.ES....------I..FTNI......-D.C
Achlya ambisexualis: H...........S.EN.----N......G...........SNM.DF..T...R..R...PT-----------TSA.SMR...T.C.S...M..TT.IAA.V----.S....------V..SS.M....VD-LS.
yeast: hsc7OA1 ..........L.H.AG.----.YT..S.S.N.....Q...TNLLE.FKA.....T-.L.I.-----------D.A...R...T...R...T...V...TV.---V.S..D.------E..ESS....R..-D..
Kluyveromyces: hsc70 ..........L.H.AG.----.YT..S.S.N.....Q...TNLLE.FKT.....T-.A.I.-----------G.A...R...T...R...T...VA..TV.---V.S..D.------E..EA.I...R..-DI.
yeast: SSA1 ..........L.F.E..----I......A...........N.L...FIQ...R..-KK...-----------TNQ...R...T.C.R...T....A..SV.---..S....------I..YTSI...R..-..C
yeast: SSA2 ..........L.S.E..----I......A...........N.L...FIQ...R..-KK...-----------TNQ...R...T.C.R...T....A..SV.---..S....------I..YTSI...R..-..C
yeast: SSA3 ..........L.S..E.----.......A...........N.L....AT...R.T-KK.I.-----------NNQ.S.R...T...R...A....S..S..---..S....------M..YTS....R..-..C
yeast: SSA4 ..........L.F..E.----.......A...........S.L..F.A....R..-KK..T-----------TNQ.S.R...T...R...T....A..S..---..S....------I..YTSI...R..-..C
Hansenula: hsp70 ..........L.S..E.----I......A...........N.L...FAN...R.Y-KK..T-----------TNQ...R...T.C.R...T....A..SV.---..S.Y..------I..YTSI...R..-..C
Cladosporium: hsp70 ..........F...EE.----I....S.A...........N.L...FSN...R.H-KK...-----------DNA...R...T.C.R...T....A..S..---..S....------I..FTSN...R..-.VG
Blastocladiella: hsp ..........L...E..----I......A......A....N.L...F.Q...R.H-KK...------------G.ARPR...T.C.R...T....A..S..---..S....------I..YTSI...R..-..C
Entamoeba: hsc70: ..........L.A....----......S............N.L...FIA...R.Y-KK.I.-----------GNA..VR...T.C.R...T...AATAN..---V.Q..D.------I..YTSI...R..-...
Trich. vagin. ..........L.D..G.----M...R..A...........S.L.DYFADR.Q..F-KC..R-----------ESP...RT..T.C.R...TI.TAANAS.L---CES.YQ.------H..QD.IS..M..-N..
Dictyostelium: ..R..-..C
T. brucei: hsp7OA4 .........TL....R.----I..................N.L.A.FT....R..K.K...-----------SNL...R...T.C.R...T...AA.AT..---......N------I..QA.I...R..-..C
T. brucei: hsc70 ..F........ISVSG.----.................V.AALLE.ALADIRNRYGIEQG.-----------LSQKM.SK..SRC.EV..V..H..VGE.A---L.G.LPD-----GEEYVLK....RL.-..C
T. cruzi: hsp7OA8 .........TL....G.----I..................N.L.A.FTD...R..K.K...-----------TNL...R...T.C.R...T...AA.AT..---.....DN------I..QA.I...R..-..C
L. major: hsp70.4 .........TL...ESG----.......A...........N.L.DYSPLSSRCAA-ARTAV-----------ATPAPRAG..T.CERV..T.....TAN..---....Y..------N..FSKI...R..-.MC
L. amazonensis: A .........TL....G.----I..................N.L.TFFT....R..K.KN.A-----------SSH.S.R...T.C.R...T...A..AT..---.....DN------V..QA.IN..R..-..C
L. amazonensis: C .........TL....G.----I..................N.L.TFFT....R..K.KN.A-----------SSH.S.R...T.C.R...T...A..AT..---.....DN------V..QA.IN..R..-..C
L. amazonensis: .........TL...ES.----.......A...........N..
Leishmania: hsp7OA3 .........TL....R.----I..................N.L.TFFT....R..K.KN.A-----------SSH...R...T.C.R...T...A..AT..---......N------I..QA.I...R..-..C
Leishmania: pseudo .........TL...ES.----.......
L. donovani: hsc70 .........TL....D.----I..................N.L.TFFT....R..K.KN.A-----------SSH...RG..T.C.R...T...A..AT..---......N------V..QA.I...R..-..C
Theileria: ..............E..----I......A...........NLL.E.C.RD.MRL.N.KNI.-----------SN....R...THC.R...V......AT..---L.S.Y..------I.YNT.IS..R..-..C
Theileria parva ..............E..----I......A...........NLL.E.C.RD.MRL.N.KNI.-----------SN....R...THC.R...V......AT..---L.S.Y..------I.YNT.IS..R..-..C
Euplotes eurystomus ..........L...EE.----I......A.N.........H.L.EFCQAD....T-K..I.-----------DNP...R...TQC.....I..TAVN.NIE---CE..A..------E.YNVS.......-...
Cryptosporidium par. ..........L...E..----I......A...........N.L.EFC.QD..R..R.M..T-----------SNA...R...TQC.R...T......AT..---L.S.Y..------I.YSVAIS..R..-..C
Babesia microti .....A........E..----I......Q...........N.L..FC.DD..R..G.KNI.-----------GNR...R...TQC.R...T..H...AT.V---VE.MY..------I.YNCNI...R..-..C
Plasmodium:hsp7OA .........L..FC..D..R..R.K...-----------.NS...R...TQC.R...T......AT..---..S....------I.YSV.VS..R..-..C
P. cynmolgi: hsp70A2 ..........L...E..----I......A...........N.L..FC..D..R..R.K...-----------.NS...R...TQC.R...T......AT..---..S....------I.YSV.VS..R..-..C
P. falcip.: hsp70A ..........L...E..----I......A...........N.L..FC..D..R..R.K...-----------.NS...R...TQC.R...T......AT..---..S....------I.YSV.VS..R..-..C
Eimeria: hsc70 .M........L...E..----I......A...........N.L.DFC.QD..R..RSK.P.-----------TNS...R...TQC.R...T......AT..---..S....------I.YSVS.S..R..-..C
Chlamy.: hsc70: ..........L...EE.----I......A...........E.L...FAN..QR.Y-KK..K-----------TSP...R...T.C.R...T...AA..T..---L.S....------V..ATSI...R..-..C
Bremia: hsp7O ..........L.S.EE.----I....S.AR..........N.L.D.FTQ...R.H-RK.IT-----------ENQ...R...T.C.R...T....A.AY..---..S..D.------I..NS.I...R..-DMC
Achlya: hsc70 ..........L...EE.----I......A...........N.L.D.FTA...R.H-RK.MT-----------QNQ...R...T.C.R...T....A.AY..---..S..D.------I..NS.I...R..-DMC
Pyrenomonas: hsp70 ..........L...EE.----I......A...........S.L..FF.S...R.Y-KK.VT-----------SNA.S.R...T.C.R...T...G...TV.---..S.ID.------I.YYASI......-..C
Ascophyllum nodosum ..........L...EE.----I....S.A...........N.L...F.A.....Y-KK.I.-----------GNS.S.R...T.C.R...A..T...SS..---..S....------I..YTSI...R..-.MC
Guillardia th. hsc70 ..........L......----I...L..A...........N.L.QFVLQ...R.H-KK.P.-----------ENA...R.I.T.C.R...T...AAN.T..---V.SC...------I..YTNI......-.PL
carrot: hsp70: ..........L...EE.----IY....PKS..........N.L..RF.T..LTN.-KK.IR-----------WECE..R...T.C.R...T.....AQTTIE--..S.Y..------V..YT.I...R..-...
spinach: hsc70 ..........L...EE.----I......A...........N.M...SLQ...R..-KK.IM-----------ETPGHIR...T.C.R...T...TA..T..---..S.Y..------V..YSPI...R..-...
petunia: hsp7O ..........L...EE.----I......A...........N.M...F.Q...R..-KK.I.-----------GNP...R...T.C.R...T...TA..T..---..S.Y..------I..YS.I...R..-...
tomato: hsc70A ..........L...EE.----I......A...........N.M...F.H...R.H-KK.IT-----------GNP...R...T.C.R...T...TA..T..---..S.Y..------V..YS.I...R..-...
tomato: hsc70B ..........L...EE.----I......A...........N.M...F.Q...R..-KK.IT-----------GNP...R...T.C.R...T...TA..T..---..S.Y..------I..YS.I...R..-...
soybean: hsp70 ..............EE.----I......A...........N.M...F.S...R..-KK.I.-----------GNA...R...T.C.R...T...TA..T..---..S.Y..------I..YA.I...R..-.M.
tobacco: hsp70 ..........L...EE.----I......A...........N.L...F.QD..R.H-KK.I.-----------GNA...R...T.C.R...T...TA..T..---..S.Y..------I..YA.I...R..-.M.
maize: hsp7OA1 ..........L...EE.----I......A...........N.M...F.Q...R..-KK.I.-----------GNP...R...T.C.R...T...TA..T..---..S....------I..TPRSS..R..-...
lily: hsp70 ..........L...EE.----I......A...........N.M...FAQ...R.H-KK.I.-----------GNP...R...T.C.R...T...TA..T..---..S.CD.------I..YS.I...R..-...
sea urchin: hsp70 ..........L.A....----....LT.A...........N.L...TSL...R.Y-KK.MR-----------TNP..IR...T...R...T....A.AN..---V.S....------I..YTSIS..R..-D.C
sea urchin: hsp70-4 ..........L.A....----....LT.A...........N.L...TSL...R.Y-KK.MR-----------TNP..IR...T...R...T....A.AN..---V.S....------I..YTSIS..R..-D.C
Trich. britovi mBiP? ..............E..----I....S.A.....V.....N.M...F.A...R..-KK.M.-----------SNP...R...T.C.R..RT......AS..---..S.Y..------I..YT.I...R..-...
Dirofilaria: hsp70 R..-..C
B. malayi: ..............E..----I....S.A......-....N.M...F.A...RND-KK..A-----------SNP...R...T.C.R...T....S.AS..---..S....------I..YTNI...R..-..C
O. volvulus: ...RND-KK..A-----------SNP...R...T.C.R...T....S.AS..---..S....------I..YTNI...R..-..C
C. elegans: hsp7OA ..............E..----I....S.A...........N.M...FCA...R.H-KK..A-----------SNP...R...T.C.R.NET....C.AS..---..S....------I..YTNI...R..-..C
Dros.: hsc70-4 ............S....----I....S.A...........N.L.T.F.Q...R.H-KKIDT-----------TN....R...T.C.R...T......AS..---..S....------T..YTSI...R..-...
D.sim.: hsc7O-1 ..........V...E..-----......A...........N.L.....Q..QR.H-.K..G-----------QN....R...T.C.R...T......AS..---..S....------V..YTSV...R..-...
Dros.: hsc70-1 ..........V...E..----I......A...........N.L...F.Q..QR.H-KK..G-----------QN....R...T.C.R...T......AS..---..S....------V..YTSV...R..-...
mosquito: hsp70-1 ...............E.S---L...R..A...........N.M.A.F.....R.F-KK...-----------.NA...R...T.C.R...T.....EAT..---....MD.------I.YYTKIS..R..-..C
mosquito: hsp70-2 ...............E.S---L...RS.A...........N.M.G.F.....R.H-KK...-----------.NA...R...T.C.R...T.....EAT..---....MD.------I.YYTKIS..R..-..C
mosquito: hsp70-3 ...............E.S---L...RS.A...........N.M.G.F.....R.H-KK...-----------.NA...R...T.C.R...T.....EAT..---....MD.------I.YYTKIS..R..-..C
Dros.: hsp7OA2 ...............E.S---L...RS.A...........N.L.T..AD...R.Y-KK..R-----------SNP...R...T...R...T.....EAT..---.......------Q..YTKVS..R..-..C
Dros.: hsp7OA4 ...............E.S---L...RS.A...........N.L.T..A....R.Y-KK..R-----------SNP...R...T...R...T.....EAT..---.......------Q..YTKVS..R..-..C
Schistosoma: ..............E..----I....S.A...........N.M.D.F.K..Q..Y-NK.IR-----------GN....R...T.C.R...T....A..NL.---..S.CD.------I..YTVI...R..-...
Hydra m.: hsp70 ............A.E..----I..I.S.A...........N.L...F.D...R.H-KK...-----------SN....R...T.C.R...T..A...ASV.---..S..D.------I..YTSI...R..-..C
Hydra o.: hsp70 ............A.E..----I....S.A..........G
sea urchin: ..V.......V...EE.----I....S.SR..........N.M.T.SSP...R.H-KK.IT-----------PN...VR...T.C.R...T......AK..---..S....------I.YYTSV...R..-...
sea urchin: hsp70-4 ..........L.A....----....LT.A...........N.L...TSL...R.Y-KK.MR-----------TNP..IR...T...R...T....A.AN..---V.S....------I..YTSIS..R..-D.C
trout: 7O.14 ...............
salamander: hsp70 .................----I......A...........N.M...FH.P..R.H-KK.IT-----------.N...VR...T.C.R...T......AS..---..S....------I..YTSI...R..-...
rat: hsc7OA ..............E..----I....S.A...........N.M...FIA...R.H-KK.I.-----------EN...VR...T.C.R...T......AS..---..S.Y..------I..YTSI...R..-...
mouse: hsc7OA ..............E..----I....S.A...........N.M...FIA...R.H-KK.I.-----------EN...VR...T.C.R...T......AS..---..S.Y..------I..YTSI...R..-...
mouse: hsc7OA* ..............E..----I....S.A...........N.M...FIA...R
hamster: hsc7OA ..............E..----I....S.A...........N.M...FIA...RND-KK.I.-----------EN...VR...T.C.R...T......AS..---..S.Y..------I..YTSI...R..-...
cow: hsc70 ..............E..----I....S.A...........N.M...FIA...R.H-KK.I.-----------EN...VR...T.C.R...T......AS..---..S.Y..------I..YTSI...R..-...
human: hsc7OA ..............E..----I....S.A...........N.M...FIA...R.H-KK.I.-----------EN...VR...T.C.R...T......AS..---..S.Y..------I..YTSI...R..-...
mouse: pseudo1 ..-...
mouse: pseudo2
human: pseudo ...S.I........E..----I..A.S.AR..........N.M.&.FIA.Y.R.H-K.HI.-----------ET....R..CT.C. TV....HATT.---..S.S..------I.IYTSSSC.S..-...
chicken: ..............E..----I....S.A...........N.M..RF.....G.H-KR.NA-----------GN...VR...T.C.R.R.T......AS..---..S....------I..YTSI...R..-...
mouse: hsc7OB ..............E..----I....S.A...........N.M.S..A....R.H-KK.IG-----------PN...VR...T.C.R...T......AS..---..S.Y..------V..YTSI...R..-...
human: hsc70B ..............E..----I....S.A...........N.M.S..A....R.H-KK.IG-----------PN...VR...T.C.R...T......AS..---..S.Y..------V..YTSI...R..-...
cow: hsp70 ..............E..----I....S.A...........N.M.S.WA....R.H-KK.I----------------.PA...T.C.R...T......AS..---..S.Y..------V..YTSI...R..-...
trout: 7O.7 ..............E..----I......A...........N.L.S.F....GR.H-KK...-----------QN....R...T.C.R...T....S.AS..---..S....------I..YTSI...R..-.MC
Xenopus: hsp7OA1 .................----I......A...........N.M...F.....R.H-KK.IG-----------QN....R...T.CDR...T....S.AS..---..S....------I..YTAI...R..-..C
mouse: hsc70C .................----I......A...........N.L.S.F.....R.H-KKDI.-----------QN...VR...T.C.R...T......ANL.---..S.Y..------I..YTSI...R..-..C
mouse: pseudo? ........L......./----I./..-PR...........N.L.S.F.R-V.R.H-KK.I.-----------QN...VR...T.C.R...T......ANL.---..S.Y..------I..YTSI...R..-..C
human: hsp7OB ..........V....A.----.......A...........N.L...FM...RR.H-.K...-----------GN....G...T.C.R...T......ATL.---..S....------V..YTSI...R..-..C
pig: hsp70 R...T.C.R...T......ATL.---..S....------V..YTSI...R..-..C
pig: hsp70com: .................----I......A...........N.L...F.....R.H-KK.Y.-----------QN...VR...T.C.R...T......ASL.---..S....------I..YTSI...R..-..C
mouse: hsp7OA1 .................----I......A...........N.L.S.F.....R.H-KK.I.-----------QN...VR...T.C.R...T......ASL.---..S....------I..YTSI...R..-..C
mouse: hsp70A2 .................----I......A...........N.L.S.F.....R.H-KK.I.-----------QN...VR...T.C.R...T......ASL.---..S....------I..YTSI...R..-..C
human: hsp7OA1 .................----I......A...........N.L...F.....R.H-KK.I.-----------QN...VR...T.C.R...T.....GASL.---..S....------I..YTSI...R..-..C
human: hsp7OA2 .....H......AV...----I...?. .....N.L.E?F.....R.H-KK.I.-----------QN...VR...T.C.R...T......ASL.---..S....------I..YTSI...R..-..C
CONSENSUS DLGGGTFDVSILTIDDG----VFEVKATNGDTHLGGEDFDQRIVNHLVEEFKKKN-GIDLS-----------KDKRALQRLREAAEKAKRELSSSTQTXIE---IDALFEG------KDFXXTLTRAKFE-ELN
*NO1
*NO2 315 33O 345 36O 375 39O 4O5 42O
*NO3 | | | | | | | |
*2nd CONSENSUS ADLFRRTLEPVEKALKDAKLDKSDIDDVVLVGGSTRIPKVQKLV-KDFFNGKEPNKGINPDEAV-AYGAAVQAGVLSGDXX----XXVKDVLLLDVTPLSLGIETLGGVMTKLIERNTTIPTKKSQIF-STAA
E. coli: hsc70 .P.VK...LACRR.....GVEADEVLE..M......V.L.RER.-GE..-.RP.LTS.D..KV.-.I...I..DI.V.NKP------DSEM.....I.....L..M..LVE.V.P......VARA.D.-T.FK
H. influenzae: hsc70 YP.VK.S.LTCRR.....NVESEEVQA..M......V.Y.REQ.-GE..-..T.LTS.D..KV.-.L...I..DI.V.NKT------DS.M.....V........M..LVE.I.P......VARA.E.-T.FK
Buchnera aph. hsc70 K..IQK..FICSNL..EID.KIES.KE.IM......V.L.YQE.-.N.S--.S.LIS....QV.-.I...I.SDM.MKNTI------Q.RTI............VM..FVE.I.F...P..IS.TKE.-T..K
B. megaterium: .G.VE.TMA..RQ.....G.SA.EL.K.I.........A..DAI-.KET-.QD.H..V....V.-.L...I.G...T..--------....V.............M...F............S...V.-....
B.subtilis: dnaK .G.VE..MA..RQ.....G.SA.EL.K.I.........A..DAI-.KET-.QD.H..V....V.-.L...I.G...T..--------....V.............M...F............S...V.-....
Bacillus stearo. .H.VE..MG..RQ..Q..G.TPE...KII.........A..EAI-.REL-....H..V....V.-.I...I.G..IA.E--------....V.............M...F............S.....-T...
Staph. aureus: DS.I...M..TRQ.M...G.TN....E.I.........A..EA.-.KEI-.......V....V.-.M...I.G..IT..--------....V............I...R.NT..........S....Y-...V
Streptococcus pyog. R..VE..KT..RQ..S..G.SL.E..E.I.........A.VEA.-.AET-......SV....V.-.M...I.G..IT..--------....V.............M...F....D.......S...V.-....
Streptococcus mutans R..VE..KT..RQ..S..G.SL.E..E.I.........A.VDA.-.AET-......SV....V.-.M...I.G..IT..--------....V.............M...F....IAIQQSQLLNHKSSQQ.LQ
Streptococcus agal. R..VE..KT..RQ..S..G.SL.E..E.I.........A.VEA.-.AET-......SV....V.-.M...I.G..IT..--------....V.............M...F....D.......S...V.-....
Streptococcus pneu. R..VE..KV..RQ..S..G.SL.E..E.I.........A.VEA.-.AET-......SV....V.-.M...I.G..IT..--------....V.............M...F....D.......S...V.-....
L. lactis: dnaK H..VEA.RQ..RQ..S..G.ST....E.L.........A.VE..-RHET-N.....SV....V.-.M...I.G..IT..--------....V.............M...F....D.......S...V.-....
Erysipelothrix: dnaK KS.VE..MV..RQ.....G.T.N.IHQ.L.........A.VEA.-.NDL-......SV....V.-.M...I.G..I...--------G.................M.....V..........S...V.-....
Mycoplasma pneum. KQ.LE..RN.ISDV.RE..IKPEE.NEIL.......M.A.....-ESMVP.HS..RS.....V.-.I...I.G...R..--------............T.S.......A.P..K......VS.....-...Q
Mycoplasma gen. KP.LE..RN.ISDVI.E..IKPEE.NEIL.......M.A.....-ESMVP..K..RS.....V.-.I...I.G...R..--------............T.S.......A.P..K......VS.....-...Q
Mycobacterium tuber. Q..LD..RK.FQSVIA.TGISV.E..H.........M.A.TD..-.ELTG.......V....V.-.V...L.....K.E--------..................K.....R...........R.ET.-T..D
M. leprae: dnaK Q..LD..RQ.FQSVV...GISV.E..H.........M.A.TD..-.ELTG.......V....V.-.V...L.....K.E--------..................K.................R.ET.-T..D
M.paratuberculosis: Q..LD..RQ.FKSVIA..GISV....H.........M.A.TD..-.ELTG.......V....V.-.V...L.....K.E--------..................K.................R.ET.-T..D
Streptomyces: dnaK S..LE.CKT.FHNVI...GIQL.E..H.........M.A.AE..-.ELTG..DA...V....V.-.I...L.....K.E--------..................K..I..............R.E..-T..E
S. griseus: dnaK ...LD.CKT.FHNVI...GIQL.E..H.........M.A.AE..-.ELTG.Q.A...V....V.-.I..SL.....K.E--------..................K..I..............R.E..-T..E
Clostridium: dnaK H..VE..INIMKE...SGNVSLN...K.I.........A..EA.-.N.T-....S..V....C.-.M...I.....T..--------............T.........A.P.........AR....L-....
Clostridium aceto.: EG.VQD.I..MK...S..G.SIN...KI..........A..EA.-.NYT-..D.S..V....C.-.I...I.....T..--------.........S..T.........A.P..........R...V.-....
Methanosarcina: E..LEK..VSMRR..S....TPN...K.I....A..M.A.VE..-EN.T-..K.Y.N.......-.I...I.....G.E--------............T........IA.P..Q.............-....
Halobacterium: E..IE...G.T.Q..A..DYT.....E.I.......M.Q..DQ.-EEMT-.Q..KRTS......-.L...I........--------.D.IV..........V.VK..LFER..DK......EE.K..-T..Q
Halobact. cut.: E..IE..VG.T.Q..E..G.S.......I.......M.Q..AQ.-E.LV-.Q..K.NV......-.L.....G.....E--------VD.IV.V..........VK..LFER...K..A...TA.KV.-T...
Thermoplasma a.: SPIVE.VKG.ID...EG...K.TE.TKLLF...P....Y.R.Y.-E.YLGI.S.EG.VD.M...-.I...I.GA..K.E--------IK.IV......VT.SV.....IA.PI.PA.....VR.....-T..E
Synechocystis: dnaK1 KQ.LED.RV.LTQ..D.GEIRA..VHR.I.........AI.RVI-QE..PDSQLERSV......-.L...I....IG.E--------.E.............L....E.T..I.........SR.EV.-...V
Synechococcus: dnaK1 SN.VQA.I..IQQ....SN.TIDQ..RIL.....S...AI.QA.-QK..G..T.DLT......I-.L...I.....G.E--------.........I.....L......F..I.........SRT.V.-T..T
Therm. aquaticus QP.LK.LRG...Q.....G.TPAQ..E.I....A..V.A..QV.-RELL-.....RSV....V.-.M...I.....M.E--------.R..V..........V..K.....V..P.......R.CE..-T..E
Deinococcus prot.: ...LK.VRQ...Q.MR..GVSS..LNE.I.........A.KRI.-..LT-.....ESV......-GL.......IIQ..SN------LG.IV.V.....T..V.VK..MIAPM.T...AV.A..TE.Y-T..E
Thermomicrobium ros: ...VEK.IP.M.Q.....G.SPR.V.E......Q..M.LI.RK.-QE..-....H.......V.-.I...I..G..A.E--------..E.........T.A.......A.PI.P.......R.....-T..S
Synechocystis: dnaK2 S..ID.CGI...N.IR...I...AL.EI..........A..EV.-.KIL-..D..Q.V....V.-.V...I.G.....E--------...I.....S.....V.........I.P..........ET.-...V
Synechococcus: dnaK2 S..ID.CRI...Q.I.....AL.E..EI..........A..AI.-.QMT-.....QSV....V.-.I...I.G...A.E--------...I...........V...........P..........ET.-....
Synechocystis: dnaK3 G..VT.LQR..KRV....G.SPVQ..E......G..M.M.KG..-RS.I-DR...ENV....V.-.I...I...I.D.E--------...I.........F.L..I....K..LP......VR..D..-..GE
Synechococcus: dnaK3 E..LE.MMR.LRR.....R.QPQ...E.........M.M..Q..-RSLI-.R...QNV....V.-.I...I...I.A.E--------...I...........L..I....K...P...A..VRR.D..-...E
Anabaena variabilis ...LG.VRN..KR.....G.RPD..EE.........M.M.KQ..-R.LI-.I..SENV....V.-.M...I...I.A.E--------F..............L.AI....K...P......VRR.D..-..SE
Aphanothece halo. RP.VEK.ID.LKQ.IE.SG.SVDQ..RIL.........E..EAL-.K.......DRS.......-.L...I.G...GQEQE------.E.L...V.I..........E.F..V.........S...V.-...S
Porphyra: cBiP: SR.IDKCSI..NN.......EA.S..E...........AI.QM.-.RLI-..D..QSV....V.-.I.........A.E--------...I...........V.........I.P..........EV.-...V
Chlamy. rein. cBiP N..LE.CKV..QQ..R....SI...QE.I.........A..EI.-RKLSG..D..VTV....V.-.L.........A.E--------.S.IV..........L...........P....L..S..EV.-....
pavlova: cBiP S...D.CRI...N.......KPNQ..E...........A.K...-..IL-.....ETV....V.-.I...I.......E--------...I...........V......T..I.P....V.....E..-...V
Odontella sin. cBiP QE.IE.CQI......T..R.E....NE...........AI.N..-ESLT-N.K..QSV....V.-.I...I...I.A.E--------I..............V.........I.A......V...EM.-...V
Cryptomonas: cBiP: S..IN.VKI...N........S.K..E...........AI.E..-.RIL-N.T..QTV....V.-.I...V.....A.E--------...I...........V......T.RI.P..........EV.-...V
Pisum sativum cBiP S..LD.LRT...NS.R....SIK...E.I.........A..E..-.KLI-..D..VTV....V.-.L.........A..--------.S.IV....S.....L.........I.P....L..S..EV.-....
Cucumis sativ. cBiP S..LD.LKT...NS.R....SFK...E.I.........A..E..-.KMT-.....VTV....V.-.L.........A..--------.S.IV....S.....L.........I.P....L..S..EV.-....
Cucumis sativ. mBiP S..LD.LKT...NS.R....SFK...E.I.........A..E..-.KMT-.....VTV....V.-.L.........A..--------.S.IV....S.....L.........I.P....L..S..EV.-....
Citrullus lan. cBiP S..LY.LKT...NS.R....SFK.VHE...........A..E..-.KMT-.....VTV....V.-.L.........A..--------.S.IV....S.....L.........I.P....L..S..EV.-....
Spinach: cBiP S..LD.LRT...NS.R....SF..L.E.I.........A.IE..-.KMT-..A..VTV....V.-.L.........A..--------.S.IV........I.L.........I.P....L..S..EV.-....
Aquifex aeolicus K.IVD..IDI.KQ..E....KP....E...........L..QRI-.E..-....H..L....V.-.M...I.....A.E--------..EIV.V........V..Y.....V..P...P..VR.CE..-T..H
C. pneumoniae: .S.IE..KS.CI...S....SAK.....L....MS.M.A.QET.-.EL.-.......V....V.-.I...I.G...G.E--------.........I..............T.V........Q.K...-....
Chlamydia: dnaK SS.IE..KQ.CAQ.......SA......L....MS.M.A..AV.-.RSL-V.SLI.AV....V.-.I...I.G...G.E--------.........I..............P.V........Q.K...-....
Chlamydia pneumoniae .S.IE..KS.CI...S....SAK.....L....MS.M.A..ET.-.EL.-.......V....V.-.I...I.G...G.E--------.........I..............T.V........Q.K...-....
Borrelia: dnaK DH.VQK.K..CL..I...G.KA...NE.I.........AI..I.-..I.-.QD....V......-.I...I.G.I.T.E--------T..MV..................................V.-....
Leptospira interrog. KS.VD..RI.C.N..R..G.KA...NE.I.....I...A..E..-.QV.-......SV....V.-.I...I.G...A.E--------.S.....................................V.-....
Helicobacter pylori: E..VEE.ISKI.GVI...G.T.NE.SE..M...........ER.-.A.I-N..L..SV....V.-.V..SI.G...K..--------.........................V.D.G....A...SQVF...E
Myxococcus xanthus T..ID..I..CRI.....GIPAQQ.NQ.L....M..M....QK.-NE..-.R..H.......V.-.V...I.G...K.E--------...............V..A...F..I.DK.....C....V.-...V
Caulobacter: dnaK D..IA..IG.C.Q.....G.K.....E.I....MS.M....QA.-Q...-.R..H..V....V.-.L.........Q..--------............T...DT....FTP...........R..T.-...D
Rhizobium meliloti: E..IQK.IA.CKA.....GVSAAE..E......M..M....ET.-.QL.-....H..V....V.-.M...I.....Q..--------......................F.V..............V.-...D
Bradyrhizobium japo. D..IQK.V..CR......GVTAGE.GE......MS.M....EV.-.QL.-....H..V....V.-.I...I.....Q..--------......................F.RI.D...........V.-...E
Rhodoba. capsulatus: D..IKAS.K.CAA.....GVS.DE..E......M..M.R.VEE.-TK..-....H..V....V.-.L...I.....Q..--------....V.................F.R..D.............-...E
Agrobacter. tum.: D..VQ..VA.CKA.....GVTAAE..E......MS.M...REV.-.QL.-....H..V....V.-.M...I.....Q..--------......................F.R..D...........T.-...E
Brucella: dnaK D.AVHA.V..CKA.....G.KAGE..E......M..M.EI..C.-.A..-....H..V....V.-.M...I.GG..Q..--------......................F.R..............T.-...E
Rhodopseudomonas sp. D..VQK.I..CR......G.TAGE.SE......M..M....EV.-.QL.-....H..V....V.-.I...I.....Q..--------......................F.RI.D...........V.-...E
Rhizobium legum.: D..VQ..IA.CKA.....GVTAAE..E......MS.M....EV.-.QL.-....H..V....V.-.L...I.....Q..--------......................F.R..............T.-...E
Pseudomonas cepacia: E..VE..I..CRI.I...GVKV......I....Q..M...MEK.-.E..-..D.RRDV......-.V...I.GQ.....--------R.........-.......V......M.SK.......HA.VY-...D
Ralstonia eutropha EE.IT..I..CRT.I...GVKV......I....M..M....EQ.-RE..-...AR.DV......-.V...I.GS.....--------RT.......................M.NK.......HA.V.-...D
Nitrosomonas europ. EE.IE..AG.CRT.....G.SV...N..I....Q..M....EK.-.EI.-....R.DV......-.I...I.G...K..--------...........................QK.......AQ.V.-...D
Franc. tularensis: S..VM.S...CK...E..G.S....TE.L....Q..M.L..EK.-.E..-....R.DV......-.V...I.G...A..--------..................M....................V.-...E
Legionella pneumo. EK.VE..I..CKT.....G.TV.Q.NE.I....Q..M.L...T.-EE..-....R.DV......-.V...I..A....E--------...I..............M.........K.......AT.V.-...D
Haemoph. actinomyc. E..VAKS....RI.....GKSP.E....I....Q..M.L..QE.-EK..-..A.R.DV......-.I.....G...A..--------..................M.....T...K..........V.-...E
Haemoph. influenzae: E..VASSI.SLKAV....DKGV.E.H.II....Q..M.L..QK.-AE..-...AR.DV......-.I.....G...K..--------..................M.....T...K..........V.-...E
Haemoph. ducreyi: E..VS.S...LKT..A..G.SV.E.N..I....Q..M.L...K.-A...-....R.DV......-.I.....G...A..--------.T................M.....S...K..........V.-...E
Pasteurella haem. E..VN.S...LKT..A..G.SVG..N..I....Q..M.L...K.-A...-..T-R.DV......M.I.....G.....S--------.T................M.....TL..K..........V.-...E
Vibrio cholerae E..VQ.S...LKV..A..D.SVN..T..I....Q..M.M...K.-AE..-....R.DV..E...-.V.....G...A.E--------......Q...L.F.....M......F..K.......AN.V.-...E
Buchnera sp. E..IL.SI..LKV.....G.SVT..N..I....Q..M.M..SK.-A...-....R.DV......-.V.....G......--------..................M..I..S..NK.......H....-...E
Salmonella typh.: E..VN.SI..LKV..Q..G.SV...N..I....Q..M.M...K.-AE..-....R.DV......-.I.....G...T..--------..................M.....P..TK.......H..V.-...E
E. coli: dnaK E..VN.SI..LKV..Q..G.SV......I....Q..M.M...K.-AE..-....R.DV......-.I.....G...T..--------..................M.....T..AK.......H..V.-...E
E. coli B: dnaK E..VN.SI..LKV..Q..G.SV......I....Q..M.M...K.-AE..-....R.DV......-.I.....G...T..--------..................M.....T..AK.......H..V.-...E
S. pombe: mBiP DP.V...ID.CKR.....N.QT.E.NE.I....M..M.R.VET.-.SI.-KR..A.SV......-.I...I.G.....H--------...LV.................F.R..N.......R...V.-....
yeast: mBiP .P.VK..VD..K......G.ST...SE.L....MS.M...VET.-.SL.-..D.S.AV......-.I.....GA....E--------.T....................F.R..P.............-....
yeast: Yel030p .P.ID..VD..K......RITA...S..L....MS.M...ADT.-.KL.-..DAS.AV......-.L...I..A....E--------VT....................F....P..S...N......-....
Trich. vagin. hBiP QH.IA..IN.CKNC....G.TPQQ.NE.I....M..M...IDS.-RE..-..D.FR.V....V.-.I..SI.GS.ML..--------H..IV.............M...FSR..P...VV......E.-T...
Trich. vagin. mBiP QH.IA..IN.CKNC....G.TPQQ.NE.I....M..M...IDS.-.E..-..D.FR.V....V.-.I..SI.GS.MR..--------H..IV.............M...FSR..P...VV......E.-T...
Nosema locust. mBiP EKIVNK.I..CK..I....V.LK..QH.I....M..M.L..RV.-EKI.-KRK.IF.VD.E...-.K.....G.I....--------.NNM.....AS.......V..IFSPI.R....L.V.ET.V.-T.SE
Vairimorpha n. mBiP REIINK.ID.CI..MR..NIT.N..KHCI....M..M.Y.R...-.KI.-.IT.STN.......-.K...L.....E.K--------...I.....V.......L....FN.I.H..S.V.F..THS.-..SE
Eimeria tenella mBiP GA.LQQSI..C..CIR..GVQ.A.LS..I....M..M...AEV.-.NI.-H...S..V......-.A...I.....K.E--------I..L.....C............F.R..N.............-....
C. elegans: mBiP G..IK..I..CRNV.H..EVKS.Q.A..L....MS.M....AT.-QEI.-..V.S.AV......-.M...I.GA..A..--------.T...................I.....T...........V.-....
Dros.: mBiP G..IK..IQ.CQ...S..EVS..E.GE.L....M..M....ST.-QEL.-.RQ.SRSV......-.V.....G...A..--------VT....................F.R..S...........V.-...S
Trypan. cruzi mBiP EK.VQ.S.G.CKQCI...AV.LKE.SE......M..M...IEA.-.Q..-.RD.FR.V......-.L.G.TLG...RR.--------..GLV..........V......F.RM.PK..........T.F....
T. cruzi: mBiP EK.VQ.S.G.CKQCI...AV.LKE.SE......M..M...IEA.-.Q..-.RD.FR.V......-.L.G.TLG...RR.--------..GLV..........V......F.RM.PK..........T.F....
Crithidia: mBiP EK.IT.STA.CKQCM...GVELKE.N.......M..M...IET.-.T..-.R..FR.V......-.L...TLG...R..--------..GLV.................F.RM.PK..........T.-....
mouse: mBiP T..IK..IA.CQ..MQ..EVS....GE.I....M..M....QT.-Q.L.-.RA.SKAV......-.I...I.G...A..--------.T....................F....N...........V.-....
Homo sapiens mBiP T..I...IA.CQ..MQ..EVS....GE.I....M..M....QT.-Q.L.-.RA.S.AV......-.I...I.G...A..--------.T....................F....N...........V.-....
Rattus sp. mBiP T..IK..IA.CQ..MQ.REVS....GE.I....M..M....QT.-Q.L.-.RA.S.AV......-.I...I.G...A..--------.T....................F....N...........V.-....
Cricet. gris. mBiP T..IK..IA.CQ..MQ..EVS....GE.I....M..M....QT.-Q.L.G-RA.S.AV......-.I...I.G...A..--------.T....................F....N...........V.-....
pea: mBiP NN.IE..KA.CKSC....NISIK.V.E.L....M..V....QV.-SEI.-..S.S..V......-.M...L.G.I.R..--------..EL.................IF.R..S...........V.-....
bean: mBiP NH.IE..KA.CKSC....NVSIK.V.E.L....M..V....EV.-LNI.-..S.S..V......-.M...I.G.I.R..--------..EL.................IF.R..N...........V.-....
potato: mBiP NH.IE..RN.CKNC....GVSLK.V.E.L....M..V....EI.-SEI.-..S.S..V......-.M...L.G.I.R..--------..EL........AR.......IF.R..N...........V.-....
Luffa: mBiP? ..-..D.R.DV......-.A...I.GS.....--------R........................M.QK......RF..T.-...D
Lycopers. per. mBiP NN.IE..RN.CKNC....GVSLK.V.E.L....M..V....EI.-SEI.-..S.S..V......-.M...L.G.I.R..--------..EL.................IF.R..N...........V.-....
Spinacia oler. mBiP GN.IE..RA.CKNC....GVSLNEV.E.L....M..V....EI.-QEI.-..S.S..V......-.M...I.G.I.R..--------...L..................F.R..N...........V.-....
Giardia: BiP I...KKSIK...QV.R....KTT...E............IRQ.L-Q.Y....AL..D..A....-.W......SI...AK-------DH....I.....T.....Q..I.TP.....SY..V...K..-..VQ
yeast: BiP L...KK..K....V.Q.SG.E.K.V..I.............Q.L-ESY.D..KAS.........-.............EEG------.E.IV....NA.T.....T.....P..K...A.........-...V
K. lactis: BiP LA..KK..K....V...SG.Q.E....I.............Q.L-EK.....KAS.........-.............EEG------.E.IV....NA.T.....T.....P..K...A.........-...V
S. pombe: BiP HGSLQEDF....QV...SN.K..E...I.............E.L-ES..-..KAS.........-.............EEG------SDNIV....I..T.....T........G...P...R.....-...V
Achlya a.: BIP E...KK..G....VM...G.K..EV.EI...........I.Q.I-.........SR.V......-.......G.I...VDD----EDTRGI.....A........V......I.N...V.......TL-..YQ
Phaeodact. tr. BiP N...KK.MG..ARVME..D.S..E..EI.............Q.I-SEY.G....S.........-.......G.I...EGG----DATSEI.........Q....V........N.G.........T.-..HQ
Plasmodium: BiP D....E.....K.V.D...YE..K..EI...........I.QII-.E........R........-.....I...IIL.EEL------.Q..V.......T.....V..I..Q..K...V.......T.-..YQ
P. falciparum: BiP D....E.....K.V.D...YE..K..EI...........I.QII-.E........R........-.....I...I.L.EEL-------Q..V.......T.....V..I..Q..K...V.......T.-..YQ
C. elegans: BiP M....A..K..Q.V.E.SD.K.D.VHEI.............Q.I-.E.......SR........-.......G..I..EED------TGEIV....N..TM....V........G...V.......V.-....
C. briggsae: BiP M....A..K..Q.V.E.SD.K.D.VHEI.............Q.I-.E.......SR........-.......G..I..EED------TGEIV....N..TM....V.......
E. granul.: BiP: N....S..K..M.VME.SG.K.E....I...........I.Q..-.E.......IR........-..........I..VED------TG.IV....C..TM....V........P...V.........-....
E. multiloc.: BiP: N....S..K..M.VME.SG.K.E....I...........I.Q..-.E...V...SR........-..........I..VED------TG.IV....C..TM....V........P...V.........-....
Aplysia: BiP: M....S.MK..KQV.E..D.KTD...EI.............Q..-.EY......SR........-.............EED------TG.LV....N..TM....V........P...V.........-....
Drosophila: BiP L....S..K..Q.V.E..DMN.K.VHEI.............Q..-....G....SR........-.............EQD------TDAIV....N..TM....V........P...V.......V.-...S
chicken: BiP M....S.MK..Q.V.E.SD.K.....EI...........I.Q..-.E.......SR........-..............QD------TG.LV....C..T.....Y........P...VV........-...S
hamster: BiP M....S.MK..Q.V.E.SD.K.....EI...........I.Q..-.E.......SR........-..............QD------TG.LV....C..T.....V........P...VV........-...S
rat: BiP M....S.MK..Q.V.E.SD.K.....EI...........I.Q..-.E.......SR........-..............QD------TG.LV....C..T.....V........P...VV........-...S
pig: BiP EI...........I.Q..-.E.......SR........-..............QD------TG.LV....C..T.....V........P...VV........-...S
human: BiP M....S.MK..Q.V.E.SD.K.....EI...........I.Q..-.E.......SR........-..............QD------TG.LV..H.C..T.....V........PS..VV...N....-...S
maize: BiP: N....K.MG..K..ME..G.E..Q.HEI.............Q.L-R.Y.D.......V......-.F.....GSI...EGG----DET..I.....A..T.....V........P...V.......V.-T.YQ
tobacco: BiP1 ...Q.L-..Y.D.......V......-.......G.I...EGG----DET..I.....A..T.....V........P...V.......V.-T.YQ
tobacco: BiP (A) N....K.MG..K..MD..G.E.TQ..EI.............Q.L-..Y.D.......V......-.......G.I...EGG----DET..I.....A..T.....V........P...V.......V.-T.YQ
tobacco: BiP (B) N....K.MG..K..ME..G.E.NQ..EI.............Q.L-..Y.D.......V......-.......G.I...EGG----DET..I.....A..T.....V........P...V.......V.-T.YQ
tobacco: BiP8 ......Q.L-..Y.D.......V......-.......G.I...EGG----DET..I.....A..T.....V........P...V..S....V.-T.YQ
barley: BiP N....K.MG..K..MD..G.E.TQ.HEI.............Q.L-R.Y.D.......V......-.F.....GSI...EGG----DET..I.....A..T.....V........P...V.......V.-T.YQ
Giardia: hsc70 I....KC.D..DRV.R.S..G.N.VH.I.............QML-R...S.R.LS.NV......-....S...AL..SYKDQAGSGAIN.I..............S.TN..T..P....V.VSRKET.-T.Y.
Achlya ambisexualis: EEC.K..E.T.L.V.S..NMKPA..TEL.............NML-SAV.G...LS.S.......-.--SVLLYK...CLVS---HDATNSL..V...........V.R.CLSCTS...A..I..TRVY-T.ES
yeast: hsc7OA1 .A..KS......QV.....IS..Q..E................L-S...D..QLE.S.......-.......GAI.T.QSTS---DET..L.....A.....VGMQ.DMFGIVVP....V..I.RRT.-T.C.
Kluyveromyces: hsc70 .A..KS......QV.....IS..Q..E................L-S...D..QLE.S.......-.......GAI.T.QSTS---DET..L.....A.....VGMA.DVFGVVVP....V..I.RRT.-T.V.
yeast: SSA1 .....S..D....V.R.......QV.EI................-T.Y.......RS.......-........AI.T..ES----SKTQ.L.....A........A........P..S..S...FE..-..Y.
yeast: SSA2 .....S..D....V.R.......QV.EI................-T.Y.......RS.......-........AI.T..ES----SKTQ.L.....A........A........P..S.......EV.-..Y.
yeast: SSA3 .....S.......V...S.....Q..EI...........I....-S.........RS.......-........AI.T..QS----TKTQ.L.....A........A..I.....P..S.......ET.-..Y.
yeast: SSA4 .....S.......V.A.S.....Q..EI................-S.........RS.......-........AI.T..QS----STTQ.L.....A........A..I.....P..S.......EV.-..Y.
Hansenula: hsp70 Q....S..D....VMR.G.....QVAEI...........I....-S..........S.......-........AI.T..TS----SKTQ.L.....A........A........P..........E..-..YS
Cladosporium: hsp70 Q....GNM..G.RT.R.D.I...SVHEI..G.............-S........C.S.......-........AI....TS---SKSTKEI.....A........A.....A..K..........ET.-..FS
Blastocladiella: hsp .....S..D....V.R...MA.NEVHEI..........RI....-S..........S.......-........AI.A..QS----EK.Q.L.....A........A.....P..K......A...ET.-..Y.
Entamoeba: hsc70: I...KS.IG...RV.Q......GS......I.........VQ.L-Q..........S.......-........AI.T.TGG----KATE.......A..T.....A.....A..P..S...A....V.-..Y.
Trich. vagin. DE...S..G..AQV.....MS...VT.I......S...R..Q.L-QE.....Q.CR.VD.....-........AIIK..NS----EA...I.....A........A.EI..V..P......A...N..-T.F.
Dictyostelium: ..V..GC.D....V...S..
T. brucei: hsp7OA4 G....G..Q...RV.Q...M..RAVH..............MQ..-S...G...L..S.......-?.......FI.T.GKS----KQTEGL.....A.T......A.....A..K.............-..YS
T. brucei: hsc70 TKI.A.C.SV.QR.....SMKVE..E........S...A..AQLREL.R..QLCSSVH......-....W..H....GYGES---SRTAGIV....V...I.V.VDD.KFDVI.R......YLATKEY-..VD
T. cruzi: hsp7OA8 GE...G..Q...RV.Q...M..RAVH..............MQ..-S...R...LK.S.Q.....-........FI.T.GKS----KQTEGL........T.....A.....S..K.............-..Y.
L. major: hsp70.4 R.Q.EKC....K.V.A..DMKPQ.V..............I.QI.-SQ..G...L.RS.......-........HI.A.GHS----SKTDGL...........V..S....SV..P..S.M.VQ.T.TY-.NN.
L. amazonensis: A G....S.IQ...RV.Q...M..RSVH...............S..-S...G...L..S.......-........FI.T.GKS----KQTEGL........T.....A.....A..K.............-..Y.
L. amazonensis: C G....S.IQ...RV.Q...M..RSVH...............S..-S...G...L..S.......-........FI.T.GKS----KQTEGL........T.....A.....A..K.............-..Y.
Leishmania: hsp7OA3 G....S.IQ...RV.Q...M..RSVH...............S..-S...G...L..S.......-........FI.T.GKS----KQTEGL........T.....A.....A..K.............-..Y.
L. donovani: hsc70 G....S.IQ...RV.Q...M..RSVH...............S..-S...G...L..S.......-........FI.T.GKS----KQTEGL........T.....A.....A..K.............-..Y.
L. brazil.: hsp70 .....-..Y.
Theileria: NEK..S..V......ESSG....S.HE............I.T.I-.N.......CRS.......-........AI...NQS----EKIQEL.....A.....L..A.....V..K.........N...-T.NE
Theileria parva NEK..S..V......ESSG...RS.HE............I.T.I-.N.......CRS.......-........AI...NQS----EKIQEL.....A.....L..A.....V..K.........N...-T.NE
Plasmodium:hsp7OA I.Y..D..I....V....MM..KSVHE............I.T.I-.E.
Plasmodium:hsp7OA** EFR..A..................N...-T.Y.
Plasmodium:hsp7OA1 .....ACRS.......-........AI....QS----NA.Q.L.....CS....L..A...............A......-T.Y.
Plasmodium:hsp7OA1* E......ACRS.......-........AI....QS----NA.Q.L.....CS....L..A...............A......-T.Y.
Plasmodium:hsp7OA2 IP..QS----NA.Q.L.....A.....L..A...............A......-T.Y.
P. chabaudi:hsp70 E......ACRS.......-........AI....QS----NS.Q.L.....CS....L..A..........-....A......-T.Y.
P. cynmolgi: hsp70A2 I.Y..D..I....V....MM..KSVHE............I.T.I-.E......ACRS.......-........AI....QS----NA.Q.L.....CS....L..A...............A......-T.Y.
P. falcip.: hsp70A I.Y..D..I....V....MM..KSVHE............I.T.I-.E......ACRS.......-........AI....QS----NA.Q.L.....CS....L..A...............A......-T.Y.
Eimeria: hsc70 M.Y..NS.V....V...SGI..RSVHE............I.Q.I-QE.......CRS.......-........AILK.VNS----SQ.Q.L.....A.....L..A......................-T.Y.
Euplotes eurystomus I....KCIP....V.T.SG.S.EQ.HEI.............Q.I-...........S.......-........AI.T.EGD----SN...L.....A........A.....A..K.............-T.Y.
Cryptosporidium par. ..Y..A..A....V....GM..RSVH...............A.I-QE.......C.A.......-........AI.N.EQS----SA.Q.L.....A.....L..A...............A..T.V.-T.Y.
Babesia microti .EM.KN..I......A..DM..KQ.HE............I.Q.I-.........C.S.......-........AI.C.EQS----SK.Q.L...........L..A.....V..P......A..E.E.-T.NE
Chlamy.: hsc70: M....KCMD....C.H...M..MTVH...............Q.L-Q.......L..S.......-........AI.T.EGG----EK.Q.L...........L..A.....V..P.........E.V.-..YS
Bremia: hsp7O G.Y..K.M...APV.R....S..QVHE..............Q.L-S..........S.....P.-....T...AI...NDSS---QKLQ.L...........L..A.....T..A....V......T.-..Y.
Achlya: hsc70 G.Y..K.M.....V.R.S..S..QVHE..............Q.L-S........C.S.......-.F..T...AI...NDSS---EKLQ.L...........L..A.....T..Q....V......T.-..Y.
Pyrenomonas: hsp70 M....G.S.....V.R.S.IS..E.H...............Q.L-I.Y.....LC.N.......-........AI.A..TS----EKM-.L.....S.....L..A.....V..K.........T.V.-..Y.
Ascophyllum nodosum M....A..D....V.R...A..KSV..I...........I.Q.L-Q.......L..S.......-........AI.K.EGG-----SKTED.........M....A.....N..P...........X.-P.Y.
Guillardia th. hsc70 L....G..D..DRV.R.S.IS.GQVHEI...........I.S.L-Q.......L..S.......-........AI.A.VDS----EKTQ.L.....A........A...............C....V.-..Y.
carrot: hsp70: M...KKCMD....C.R.S.I..AQVHE..............Q.L-Q.......LC.S.......-........AI...EGN----ERS-.L...........L..A.....V..P.........E...-..YS
spinach: hsc70 I....KCM.....C.R...M...TVH...............Q.L-Q.......LC.S.......-........AI...EGN----EK.Q.L...........L..A.....V..P.........E.V.-..YS
petunia: hsp7O M....MCM.....C.R...M...SVH...............Q.L-Q.......LC.S.......-........AI...EGN----EK.Q.L...........L..A..G..V..P.........E.V.-..YS
tomato: hsc70A M....KCM.....C.R...M...TVH...............QVAMTN......LC.S.......-........AI...EGN----EK.Q.L...........L..A.....V.IP.........E.V.-..YS
tomato: hsc70B M....KCM.....C.R...M...TVH...............Q.L-Q.......LC.S.......-........AI...EGN----EK.Q.L...........L..A.....V.IP.........E.V.-..YS
Arabidopsis: 1 M....KCM.....C.R...M...TVH...............Q.L-Q.......LC.S...V...-V......GAI...EGN----EK.Q.L...........L..A.....T..P.........E.V.-..YS
soybean: hsp70 M....KCM.....C.R..L..L.QVHE.............HQ.L-Q.......LC.S.......-........AI...QGD----EK.Q.L...........L..A.....V..P.........E...-..YS
tobacco: hsp70 M....KCM.....C.R...I...HVH...............Q.L-Q.......LC.S.......-.......AAI...EGD----QK.Q.L...........L..A.....V..P.........E...-..YS
maize: hsp7OA1 M....KCM.....C.R...M...SVH...............Q-L-Q.......LC.S.......-........AI...EGN----ERS-.L...........L..A.....V..P.........E.V.-..YS
lily: hsp70 I....KCMD....C.T...M...SVH...............Q.L-Q.......LC.S.......-........AI...ERN----EK.Q.L...........L..A.....I..P.........E.V.-..YT
Lupinus p.: ..V..P.........E.V.-..YS
sea urchin: hsp70 S....KC.....R.IL...I..KK..T............I...L-QE.L....L..S.......-........AI....KS----DEIK....V..A........A....S.I.....RV...A..T.-T.YS
sea urchin: hsp70-4 S....KC.....R.IL...I..KK..T............I...L-QE.L....L..S.......-........AI....KS----DEI.....V..A........A....S.I.....RV...A..T.-T.YS
Trich. britovi mBiP? .....S.........R......AV.HE................L-Q.......L..S.......-........AI...EKH----EA.Q.LC.............A.....A..K........V..V.-T.Y.
Dirofilaria: hsp70 .....S.MD......R...M..AQVH.I...............L-S...S...L..S.......-........AI....KS----EA.Q.L.....A........A.....A..K........T..T.-T.YS
B. malayi: .....S.MD......R...M..AQVH.I...............L-S...S...L..S.......-........AI....KS----EA.Q.L.....A........A.....A..K........T.E..-T.YS
Brugia pahangi: LS.MD......R...M..AQVH.I...............L-S...S...L..S.......-........AI....KS----EA.Q.L.....A........A.....A..K........T....-T.YS
O. volvulus: .....S.MD......RV..M..AQVH.I...............L-S...S...L..S.......-........AI....KS----EA.Q.L.....A........A.....A..K........T..T.-T.YS
C. elegans: hsp7OA .....S.MD....S.R...M...QVH.I...............L-S.L.S...L..S......L-........AI....KS----EA.Q.L.....A.....I..A...M.A..K........TA.T.-T.YS
Dros.: hsc7O-4 .....S.MD......R.......V.H.I.............R.L-Q.L.....L..S.......-........AI.H..KS----QE.Q.L..............A....SV..K........QT.T.-T.YS
D.sim: hsc7O-1 G....G.
Dros.: hsc70-1 G....G.M...A...R...M..GQ.H.I.............R.L-Q.......L..S.......-........AI.H..KS----EA.Q.L..............A.....T..K........QT...-T.Y.
mosquito: hsp70-1 S....S..Q......S...M...S.H.I.............S.L-QN..A..SL.LS.......-........AI....KD----DKIQ....V..A........A...........SR..C.QTK..-..Y.
mosquito: hsp70-2 S....S..Q......S...M...S.H.I.............S.L-QN..A..SL.LS.......-........AI....KD----DKIQ....V..A........A...........SR..C.QT...-..Y.
mosquito: hsp70-3 S....S..Q......S...M...S.H.I.............S.L-QN..A..SL.LS.......-........AI....KD----DKIQ....V..A........A...........SR..C.QT...-..Y.
Dros.: hsp7OA2 .....N..Q......N...M..GQ.H.I.............S.L-Q...H..NL.LS.......-........AI....QS----GKIQ....V..A........A...........CR..C.QTKT.-..Y.
Dros.: hsp7OA4 .N...N..Q......N...M..GQ.H.I.............S.L-QE..H..NL.LS.......-........AI....QS----GKIQ....V..A........A...........CR..C.QTKT.-..YS
Schistosoma: .....G..D......R...M...Q.H.I...............L-Q.......L..S.......-........AI....KC----EA.Q.L.....A.....L..A.....A..K........QT.T.-T.YS
Hydra m.: hsp70 I....G..G..??????????....HE..............S.L-QE......L..S.......-........AI.A..KH----EA.Q.L.....A........A...F.P..K....V...Y..V.-T.YS
sea urchin: S....G......N..R......EK.HEI...........I...--Q...E...L..S.......-.IVQ
salamander: hsp70 .....GP........R......AQIHEI...........I...L-Q.....R.L..S.......-........AI.M..KS----ENLQ.L.....A.....L..A.....V..K..S.....QT...-T.YS
rat: hsc7OA .....G..D......R.......Q.H.I...........I...L-Q.......L..S.......-........AI....KS----EN.Q.L..............A.....V..K........QT.T.-T.YS
mouse: hsc7OA .....G..D......R.......Q.H.I...........I...L-Q.......L..S.......-........AI....KS----EN.Q.L..............A.....V..K........QT.TL-T.YS
hamster: hsc7OA .....G..D......R.......Q.H.I...........I...L-Q.......L..S.......-........AI....KS----EN.Q.L..............A.....V..K........QT.T.-T.YS
cow: hsc70 .....G..D......R.......Q.H.I...........I...L-Q.......L..S.......-........AI....KS----EN.Q.L..............A.....V..K........QT.T.-T.YS
human: hsc7OA .....G..D......R.......Q.H.I...........I...L-Q.......L..S.......-........AI....KS----EN.Q.L..............A.....V..K........QT.T.-T.YS
mouse: pseudo1 .....G..D......R.......Q.H.I...A.......I...L-Q.......L..S.......-./......AI....KS----EN.Q.L..V...........A....KV..K........QT.T.-T.YS
human: pseudo1 ....LS..D......R.......Q.H.IA......H...I...L-Q...H../L..S.......-........AI....KS----EN.Q.L....I.........A.R.I.D..KH.......QT.T.-T.YS
chicken: .....G.........R......GQ.QEI...........I...L-Q.......L..S.......-........AI.M..KS----EN.Q.L..............A.....A..K........QT.T.-T.YS
mouse: hsc7OB .....G.........R......GQ.QEI...........I...L-Q.......L..S.......-........AI.I..KS----EN.Q.L..............A.....P..K........QT.T.-T.YS
human: hsc70B .....G.........R......GQ.QEI...........I...L-Q.......L..S.......-........AI.I..KS----EN.Q.L..............A.....P..K........QT.T.-T.YS
cow: hsp70 .....VP........R......GQ.QEI...........I...L-Q.......L..S.......-........AI.I..KS----EN.Q.L..............A.....P..K........QT.T.-T.YS
trout: 7O.7 S....G.........G...M..AQ.H.................L-Q.....R.L..S.......-G..L.I..AI....KS----EN.Q.......A........A..
Xenopus: hsp7OA1 S....G.........R.......Q.HEI...............L-Q.....R.L..S.......-........AI.M..KS----EN.Q.......A.....L..A.....V..K........QT.S.-T.YS
mouse: hsc70C .....G.......S.R...M..AK.H.I...............L-Q.Y...RDL..S.......-........AI.M..KS----EK.Q.L.....A.....L..A.....V..K..S.....QT...-T.YS
human: hsp70B S....S.........R......AQ.H.................L-Q.......L..S.......-........A..M..KC----EK.Q.L.....A.....L..A.....T..Q..A.....QT.T.-T.YS
pig: hsp70 S....S.........R......AQ.H.I...........I...L-Q.....R.L..S.......-........A..M..KC----EK.Q.L.....A.....L..A.....T..Q..A.....QT.N.HYLLQ
pig: hsp70com: S....S.........R......AQ.H.L...............L-Q.....RDL..S.......-........AI.M..KS----EN.Q.L.....A.....L..A.....A..K..S.....QT...-T.YS
mouse: hsp7OA1 S....G.........R...M..AQ.H.L...............L-Q.....RDL..S.......-........AI.M..KS----EN.Q.L.....A.....L..A.....A..K..S.....QT.T.-T.YS
mouse: hsp70A2 S....G.........R...M..AQ.H.L...............L-Q.....RDL..S.......-........AI.M..KS----EN.Q.L.....A.....L..A.....A..K..S.....QT.T.-T.YS
human: hsp7OA1 S....S.........R......AQ.H.L...............L-Q.....RDL..S.......-G.......AI.M..KS----EN.Q.L.....A.....L..A.....A..K..S.....QT...-T.YS
human: hsp7OA1* ..S.......-........AI.M..KS----EN.Q.L.....A.....L..A.....A..K..S.....Q
human: hsp7OA2 S....?.........R......AQ.H.L...............L-Q.....RDL..S.......-........AI.M..KS----EN.Q.L.....A.....L..A.....A..K..S.....QT...-T.YS
human: hsp7OA2* ..S.......-........AI.M..KS----EN.Q.L.....A.....L..A.....A..K..S.....Q
CONSENSUS ADLFRRTLEPVEKALKDAKLDKSDIDDVVLVGGSTRIPKVQKLV-KDFFNGKEPNKGINPDEAV-AYGAAVQAGVLSGDXX----XXVKDVLLLDVTPLSLGIETLGGVMTKLIERNTTIPTKKSQIF-STAA
*NO1
*NO2 435 45O 465 475 49O 5O5 52O 535 55O
*NO3 | | | | | | | | |
*2nd CONSENSUS DNQPGVTIQVYEGERA-MTKDNKLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVSAKDK-GTGKENKITITNDKGRLSKEEIERMVNDAEKFAEEDKKRKERVEARNELESYVYSXEKTLKD---EKLGGK
E. coli: hsc70 .G.TAMS.H.MQ...E-LVQ.CRS.AR.A.R...AL.A.GAH.R...QV..D.L.S.T.ME.-S..V.AS.QVKPSY.-.TDS..AS.IK.SMSY..Q.V.ARMLA.QKV.AARVLE.LHGA.AA-----DAAL
H. influenzae: hsc70 .G.TAM.VH.LQ...E-LVD.CRS..R.TLR....MAA.AAH.R..YQV..D.L.SVT.ME.-S.KVQAS.Q.KPSY.-.TD..VTA.IKSSFDN.Q..LQAR.LA.QRV.ADRVIE.VIVA.QA----DGAEL
Buchnera aph. hsc70 ...TIIV.HILQ...E-LV..CIS.SR.I.KD..SKKA..VR.L...E..TD.LIS.KILE.-FSH..K..Q.E.VTF-.QNKN.HKIIKESTIN.K..YYLRV.K.KKI.SKYILDLLNNA.QE-----DRNL
B. megaterium: .S.TA.D.H.LQ...P-.SA...T..R.Q..D.............S....K...V..R...L-..N..QA...KSST.-..DD..D...KE..EN.DA..Q...EV.L...ADQL.FTT......-----.E..
B.subtilis: dnaK .S.TA.D.H.LQ...P-.SA...T..R.Q..D.............S....K...V..R...L-..N..QA...KSST.-..DD..D...KE..EN.DA.KQ...E..L...ADQL.FTT......-----.E..
Bacillus stearo. ...TT.D.H.LQ...P-.AA...T..R.Q...Y.....................VH.R...L-..N..QS...KSSS.-..E...Q..IKE..EN..A.R....AA.L...ADQLIFTT.....E-----VE..
Staph. aureus: ....S.DVH.LQ...P-.AA...T..R.Q..D....E..K..........K...V..T...L-..N..QR...QSSSS-..D...D...K...VN..A....R.E.DL...AD.L.FQV....T.-----..EN
Streptococcus pyog. ....A.D.H.LQ...P-.AA...T..R.Q..D..A....I..........K...VS.K...L-..Q..QH.V.KSND.-..E...D..MK...AN..A.A....E.DLK..VDQAIFAT...I.E-----TE..
Streptococcus mutans ITNQS.D.H.LQ...P-.AA...T..R.Q..D..A...............K...VS.K...L-..Q..QT.V.QSNS.-.TD...DK.MK...AN..A.A....E.DLK..VDQAIFTT...I.E-----TE..
Streptococcus agal. ....A.D.H.LQ...P-.AA...T..R.Q..D..A....I..........K...VS.K...L-..Q..QH.V.QSNS.-.TD...DK.MK...AN..A.A....E.DLK..VDQAIFAT...I.E-----TE..
Streptococcus pneu. ....A.D.H.LQ...P-.AA...T..R.Q..D..A....I..........K...VS.K...L-..Q..QT.V.QSNS.-.TD...D..MK...AN..S......E.DL...VDQAIFAT...I.E-----TE..
L. lactis: dnaK ....A.D.H.LQ...P-.AA...T..R.Q..D..A....I..........K...VS.K...L-..Q..QT.V.KSNS.-..D...DK.MK...AN.DA.A....E.DT...ADAL.FQT......-----.E..
Erysipelothrix: dnaK ....A.D.N.LQ...P-.A....S..L.K.D..A..K..I..........V...V....M..-..N.KQS...S.SS.-..D.......RE..EN.S..LRL..EA.LK.RA.QFIHQIDES.AS-----EDSP
Mycoplasma pneum. ...ES.DVV.CQ...P-.AR...S..R.N.G..Q...K.K....I..SL........K...L-T.Q...S...SDNGN-..E...QK.IR...ANK.R.NVIR..I.L...G..I.STIKEI.QS----PEAKD
Mycoplasma gen. ...ES.DVV.CQ...P-.SR...S..R.N.G..Q...K.K....I..SL........K...L-T.Q...S...SDNGN-..E...QK.IR...ANK.R.NIIR..I.L...G.GI.NTIKEI.AS----PDAKN
Mycobacterium tuber. ....S.Q....Q...E-IAAH.....S.E..........I..............VH.T....-......T.R.QEGS.-....D.D..IK...AH....R..R.EADV..QA.TL..QT..FV.EQREAEG.S.
M. tuberculosis: EF..S.Q....Q...E-IAAH.....S.E..........I..............VH.T....-......T.R.QEGS.-....D.D..IK...AH....R..R.EADV..QA.TL..NT..FV.EQRE--G.S.
M. leprae: dnaK ....S.Q....Q...E-IASH.....S.E.........................VH.T....-......T.K.QEGS.-......D...K...AH....R..R.EADV..QA.TL..QT..FV.EQRETEN.SR
M.paratuberculosis: ....S.Q....Q...E-IAAH.....S.E.........................VH.T....-......T.K.QEGS.-......D..IK...AH....R..R.EADV..QA..L..QT..FV..QREAEG.S.
Streptomyces: dnaK ....S.Q....Q...E-IAAY..K..M.E...L............A........MH.T...L-.....Q.M.V.GGSS-.P.D.VD..RQE...Y....HA.R.AA.S..QG.QL..QT..F...-----NED.
S. griseus: dnaK ....S.Q....Q...E-IAAY..K..M.E...L............A........MH.A...L-.....Q.M.V.GGSS-.P.D.VN..REE...Y....HA.R.AA.S..QG.QL..QT..F...-----NED.
Clostridium: dnaK ...TS.E.H.VQ...Q-.AA...T..R.T.S..A.....I.....A........VK...T..-A....AN....ASTN-..DA..DKA.KE..Q..........AI.VK.NA.QT..QT....NE-----..D.
Clostridium aceto.: ....S.E.NIVQ...K-.AA...S..R.T.D..A....................V.......-.....SH....ASTN-..D...DKA.K...........K..NI.VK.NADQI.FQTD.A...-----..D.
Methanosarcina: ....S.E.H.LQ...G-IRSE..T..R.I.D........I...............H.....L-....QQS.S.QKPG.-..DD......K...MH....R....E..I..NA.ALINAA...I.E------A.D
Halobacterium: ...TQ.Q.R.FQ...E-IAEE.E...R.A.S......A.T.....S.N..E...V..E.E..-.S.NKED...EGGA.-..DDQ..E.QQE..QH....EQ.RDGI.....A.AS.RRA.TL.L.-----ENEE
Halobact. cut.: ...TS.Q.R.FQ.D.E-IRSE.....D.I........A.TD......E...DA.V..E.E.Q-.S.QRES...EGGA.-..DD..DE.QE...AH....EQ.RR.I.....A.TGIQRA.SL..E-----NEEL
Thermoplasma a.: .M.TT...H.VQ...P-LA...VS..M.N....A..............MHS......T.V..-A...KQG....ASTK-.........KKE..QY..Q.R.A..QI.LL.NA..LA..V..S..H-----A.D.
Synechocystis: dnaK1 .G.TS.E.H.IQ....-.AR...S....L.A........M.....S.E..V....K...Q.Q-.....QS.V.SHTG.-..GG...K.RQE.QQY.AQ.QL.LRMM.LQ.QAD.LFHTY.T..QE-----S.EL
Synechococcus: dnaK1 .G.VM.EVH.LQ....-LV....S..R.Q...............LA.G...D.......R.R-...RAQG.R..STG.-.TGD...A.RR...LYQ.A.QINLQMI.L.TQF.NLR..F.S..QN-----NREL
Therm. aquaticus H..TA.E.H.LQ...P-.AQ...S..R.R.E....M.A.......C.........H.T..ER-S..R.AS...Q.TTT-..E...Q.IIEE.KRH....RR.R.HA.LK.A.D.ARVQA.RV.QE-----RQ.A
Deinococcus prot.: N.....E.N.LQ...P-.AA...S..R.K.E....M.A.Q.................T..E.-T....SS...E.TTT-.D.SDV....KE..QN..A..A......K..A.DQMRVQALQQIDE-----NAVR
Thermomicrobium ros: ...TQ.E.H.VQ...P-.AA...T..R.I.D..........K.............T...R..-A..R.Q.....AST.-.TE...Q..IRE..EH....RAKR.AIDV..QA.ALL.QA....NE---FSDRIP
Synechocystis: dnaK2 .G.SN.E.H.LQ...E-.AN...S..T.R.D..........................T...R-.....QS.S..GAST-.PDT.VD...KE..SN.AA..E.R.KIDRK.QAD.L..QA..QITE-----..D.
Synechococcus: dnaK2 .G.TN.E.H.LQ...E-.AS...S..T.R.D..............I...........T....-.S...QS.S..GAST-..DN.VD...K...AN.AA..E.R..IDLK.QADTL..QS..Q.SE-----..D.
Synechocystis: dnaK3 N..TV.EVH.LQ...E-.AS..IS..R.K.S...........VQ.S.........Q.T.R..-T..R.QS..VQGASI-..EG.VN..IQE..T..AQ.RE.R..I.K..SAKALTDQAQRR..E-----VTLD
Synechococcus: dnaK3 N..TM.E.HILQ...Q-LAEG..S..R.K.............VQ.S.........Q...L..-T..R.QTV..QGAST-..Q..VK..MK...LY.QQ.RQL.A.I.K..RAQTLIAQS.RR.RE-----ISLD
Anabaena variabilis N..NS.E.H.VQ...E-.AG...S..R.K.Y........I...Q.A.........Q.T.L.R-T..R.QS...QGAST-..ES.VN..IQE.Q.Y.DV.RE......K.TRS.ALILQG.RQ.RE-----VALE
Aphanothece halo. .G.TS.E.H.LQ....-.AN...T....L.....A..........S....V....K...E..-...R.QG.V.KETG.-..QQ.....QQE..QY.D..R..MQ.I.LS.QAD.LF..H.A....-----NQGL
Porphyra: cBiP: ....N.E...LQ...E-L.....S..T.R.D..M.....................S.K..E.-A....QS...SGAST-.P.DDV....KE..ENFDV.Q..RKNIDI..QA..LC.QS..QV.E-----FED.
Chlamy. rein. cBiP .G.TS.E.N.LQ...E-FAR...S..T.R.D..............K.........S.T.T..-..S.KQD.R..GAST-.D.GDV....KE.....G.....R.S..TK.QA.TM..QT..Q..E-----FE..
pavlova: cBiP ....N.E.H.LQ...E-FAR...S..T.R.D..L.....I...............S.T.Q..-..S.QQS...SGAST-.P...V.K..KE..QN.AA..EKG.NIRVK..ADL.C.QA..QISE-----.PEA
Odontella sin. cBiP E..TN.E.H.LQ...E-LVAG..S..N.R.D...A.E............NVD.L.S.K..EL-E..I.QSV..QGASN-.DES.VSD.LAE...Y.ALN.EKRQNIDLK.QA.TLCFEA..E.DL-----FKTS
Cryptomonas: cBiP: ....N.E.H.LQ...E-FA....S..T.R.D..L.....................S.T....-.....QS....GAST-.PSD.V.....E.QNS.K...EKRDKIDLK.QSD.LC.QS..Q..E-----.E..
Spinach: cBiP .G.TS.E.N.LQ...E-FVR...S..S.R.D...........V..K.........S.T.I..-....KQD....GAST-.PGD.V....SE.....K...EKR.VIDTK.QAD.V..QT..Q..E-----..E.
Pisum sativum cBiP .G.TS.E.N.LQ...E-FVR...S..S.R.D..............K.........S.A.I..-....KQD....GAST-.PGD.V....SE..R.SK...EKR.AIDTK.QAD.V..QT..Q..E-----..E.
Cucumis sativ. mBiP .G.TS.E.N.LQ....-FVR...S..S.R.D..............K.........S.T.I..-.S..KQD....GAST-.PSD.V....SE.D...K...EKRDAIDTK.QAD.V..QT..Q.KE-----..D.
Cucumis sativ. cBiP .G.TS.E.N.LQ...E-FVR...S..S.R.D..............K.........S.T.I..-.S..KQD....GAST-.PSD.V....SE.D...K...EKRDAIDTK.QAD.V..QT..Q..E-----..D.
Citrullus lan. cBiP .G.TS.E.N.LQ...E-FVR...S..S.R.D..............K.........S.T.I..-....KQD....GAST-.PSD.V....SERR...K...EKRDAIDTK.QAD.V..QT..Q..E-----..D.
Aquifex aeolicus .Y.TE.E.H.LQ...P-LA....S.A..Y............K...C.....D...H.T...L-.....QSVRVEISS.-.TP.....IIKE..EH....R.K..LI..K.Q.DHL..QL..A..E-----A.D.
C. pneumoniae: ....A...V.LQ...P-.A....EI.R....D.......H.....S........FH.....V-AS...Q..R.EASS.-.QED..Q...R...INK......R.ASD.K..AD.MIFRA..AI..-----YKEQ
Chlamydia: dnaK ....A...V.LQ...P-.A....EI.R....D.......H...............H.....A-AS.R.Q..R.EASS.-.KED..QQ.IR...LHK....Q...ASDVK..ADGMIFRA..AV..-----YHD.
Chlamydia pneumoniae ....A...V.LQ...P-.A....EI.R....D.......H.....S........FH.....V-AS...Q..R.EASS.-.QED..QR..R...INK......R.ASD.K..AD.MIFRA..AI..-----YKEQ
Borrelia: dnaK ...TS.D.K.LQ...E-.AAQ.RI..N.I.D...A..........S........VH.....M-.....Q..R.ESSS.-..ES..D...K...AH......L..NI..K.TAN.LI.QT..S..E-----YSE.
Leptospira interog. ...SA.S.H.LQ...E-.ASA.RT..R...I.......................VH.....L-.....Q..R.ESSS.-..ED..QK..K...AH.AA..AQR.VI..K...DTLT..L...VNE-----A.D.
Helicobacter pylori: ....AGVPYGFQ...E-LAR...S......Q..A.....................T...Q..-N...SQE.K.SGSS.-.TIA--KK..K...LHK...ARK..VI....HAD.LAHQTQ.S.DE-----HKTN
Myxococcus xanthus ....L.SVH.LQ...E-.AA...T.AR.E.V..............S........VH.....L-....VQQVRVVSNS.-..EA..QA.IS..QSH.SD...K..LA.L..NADGLI.TT..S.EE-----YASL
Caulobacter: dnaK ...SA...R.FQ....-.AQ...MQ.Q...V.......................VQ.H....-A.N..QS.R.QANG.-..DSD.....KE..ANKAA.E.K.QL...K.QG.AILH.T..A.AE-----H.D.
Rhizobium meliloti: ...SA...R.SQ...E-.AA......Q...V.......................VQ......-.....HQ.R.QASG.-..DA...K.VK...AN..A....R.G...K.QAE.L.H.S..S.QE-----H.D.
Rhodoba. capsulatus: ...NA...R.FQ...E-.AA...M..M.N.EN......................VS.K....-.....QQ...QASG.-..DDD..K.IK...AN..A......L...K.TG..LLH.TR.S.EE-----H.D.
Agrobacter. tum.: ...SA...R.SQ...E-.AQ......Q...V.L..S..A...............VQ......-.....QQ.R.QASG.-..DAD..K..K...AN..A....RAG...K.QA..LIH.T..SV.E-----Y.D.
Brucella: dnaK ...SA...R.FQASVK-LAA......Q...V.........R.............V......G-.VP..HQ.R.QASG.-..DAD..K..K...AN..A....R.S...K.QA..L.H.T..S.AE-----Y.D.
Leptospira interrog. ...SA.S.H.LQ...E-.ASA.RT..R...I.......................VH.....L-.....Q..R.ESSS.-..ED..QK..K...AH.AA..AQR.VI..K...DTLT..L...VNE-----A.D.
Rhodopseudomonas sp. ...NA...R.FQ...E-.AA...M..Q...M........M..............V.......-A....QQ.R.QASG.-..DS..DK..K...AN.A.....R.A.D.K.HADAL.H.T..A.AE-----H.S.
Rhizobium legum.: ...QA...R.SQ...E-.AA......Q...VCL..S...M..............VQ......-.....QQ.R.QASG.-..DAD..K..K...AH.T.....R.A.....QA..LIH.S..S...-----Y.D.
Bradyrhizobium japo. ...NA...R.FQ...E-.AA...M..Q...M........M..............V.......-A....QQ.R.QASG.-..EAD..K..K...AN..A....R.A.T.K..ADGL.H.T..A.AE-----H.S.
Pseudomonas cepacia: ...GA...K.FQ...E-.AAG.....E.N.E........................H.G....-A.........KANS.-..EA..DQ.IK...AN.A..HNVR.LADS..QGDAL.H.TK.A.TE-----Y.D.
Ralstonia eutropha ....A...K..Q...E-.ATG..M..E.N.E..A.....T.....S.........H.G....-A.....R...KANS.-..ED..Q...K...AN.....RAR.LAD...QADALIH.TR.A.GR-----VRRE
Nitrosomonas europ. ...TA...H.LQ...E-VASG..S..Q.N..D..S....M.....I.........H......-A.......K.QASS.-..ED..QK..K...AH......ALDL.NS..QCDAMIH.VR.S.AE-----Y.D.
Franc. tularensis: ....A...H.LQ...E-.ASA..S..R...AD.......M......................-A....QN.V.KSSS.-..E.D..K..Q...AN..A...FHDL.T...TADNLIH.SR.AIQE-----..D.
Legionella pneumo. ...TA..VH.LQ...E-QASA..S..R...RD..............................-A...AQS.V.KASS.-..E..VAA..K..QSH......F..MA.L..QAD.LIH.C..SM..-----.ADE
Haemoph. actinomyc. ...SA...H.LQ...K-QASA..S..Q.N.E..N.V...M...........D..IH......-.....QQ...KSSS.-..D...QQ..R...AN..S.R.FE.L.Q...QADHLIH.TR.Q.DE-----A.D.
Haemoph. influenzae: ...SA...H.LQ...K-RAA...S..Q.N.E..N.....M.............VI.......-N....QQ.R.QASS.-..D...QQ..R...AN.DA.R.FE.V.Q...QADGIAHATR.QIAE-----A.DA
Haemoph. ducreyi: ...SA...H.LQ...K-RAA...S..Q.N.E..N.....M..............I.......-N....QQ.K.QASS.-..D..V.Q..R...AN..S.RQFE.L.QT..QAD.IAHATR.QISE-----A.DA
Pasteurella haem. ...SA...H.LQ...K-RAA...S..Q.N.E..N.....M..............I.......-N....QQ.K.QASS.-..DA.V.Q..R...AN..A...FE.L.QVC.QADGIAHATR.QITE-----A.DA
Vibrio cholerae ...SA...H.LQ...K-QAMY..S..Q.N.E..N.....M.....I..L..D...H......-Q....QK.T.QASG.-..DA...K..QE..ANK.A...FE.LATA..QADQMIHATR.QITE-----A.ER
Buchnera sp. ...SA...H.LQ...K-RSS...S..Q.N.D..N.....TA.........SD...H......-K....Q....KASS.-.NE...KK......ANS.A.Q.FE.LIQT..QGDQL.H.IK.Q.NE-----NKNS
Salmonella typh.: ...SA...H.LQ...K-RAS...S..Q.N.D..N.....M...........D...H......-NS...Q....KASS.-.NE...QK..R...AN..S.R.FE.L.QT..QGDHLLH.TR.QVEE-----A.D.
E. coli: dnaK ...SA...H.LQ...K-RAA...S..Q.N.D..N.....M...........D...H......-NS...Q....KASS.-.NED..QK..R...AN..A.R.FE.L.QT..QGDHLLH.TR.QVEE-----A.D.
E. coli B: dnaK ...SA...H.LQ...K-RAA...S..Q.N.D..N.....M...........D...H......-NS...Q....KASS.-.NED..QK..R...AN..A.R.FE.L.QT..QGDHLLH.TR.QVEE-----A.D.
S. pombe: mBiP .G.TA.E.R.FQ...E-LVR....I.N.Q....A...K.Q.....S..V..D..I....R..-A.N.DSS..VAGSS.-.TDS...A..A....YRAS.MA...AI.NG.RA..VCTDI.SN.DI-----HKD.
yeast: mBiP AG.TS.E.R.FQ...E-LVR....IGN.T.A......K.............D..I....R..-A.N.DSS..VAGSS.-..EN...Q........KSQ.EA..QAI.TA.KADQLANDT.NS..E-----FE..
yeast: Yel030p SG.TS.EVK.FQ...E-LV.....I.N.T.A......K.T..............I......L-ASH.DSS..VAGAS.-..DT..D....E..RYKNQ.RA.RNAI.TA.KADQLANDT.NSI.E-----FE..
Trich. vagin. hBiP .G.TH.N.R.FX...D-LVEG.....E.T.V..........K..........S.VH......-Q.N..QQM..QSNTA-AFLRKKSLRWSRLRNTQLKTRRSVRSSRRSTLWNSTSTRSRRQFLK-----TKRS
Trich. vagin. mBiP .G.TH.N.R.FQ...D-LVEG.....E.T.V..........K..........S.VH......-Q.N..QQM..QQHG.-..QD..AK.IQ....H.A....KR.IL.KKYAMKQ.INEI...ISE-----NEK.
Nosema locust. mBiP ...TE.D.NI.Q...P-LV.Y..F..SLK.KN..R..KN..K.....ES.....CKIT.RDA-L.K..QSLELLPSG.-.T.S.....IK...TKKIC.Q.TVYV..SK.K.RKFIEECDAIHA.-----VK.V
Vairimorpha n. mBiP ...SKLD.RI.Q...K-.V....Y..AIK.KN..S..A.I.K.....EA.....YK...Q.G-ISKNKQE.E.VPSS.-.NE...KQ..KE.KENE.KDERSQKIA.VKI.IRKILEKRNIKEGR-----DKLE
Eimeria tenella mBiP ...TQ.G.K..Q...E-.ASA.....Q...V.................V.....M.I..V..-S.A.RQQ...QSSG.-..EAQ.KQ..E...R.KD..QRQ.DL.A.K..A.TL...V..QIS.-----.KD.
C. elegans: mBiP .G.TQ.Q.K.FQ...E-.ATS.....Q.S.V..
Dros.: mBiP .G.TQ.E.K.HQ...E-.AN......S.T.V..............V........VH......-.....QQ.V.QSSG.-...D...N.IKK..EY.TA..QKR.LI.IV.QG..I.HDT.TKMEE-----FKSQ
Trypan. cruzi mBiP F..TQ.G.K.FQ...E-.AA..QMM.Q...V..................EP...CH.T....-A...TQN....ASG.-....Q....IR.S.SH..S.RLKR.L..V..NA.TQANTA.RQ.TE-----WKYV
T. cruzi: mBiP F..TQ.G.K.FQ...E-.AA..QMM.Q...V..................EP...CH.T....-A...TQN....ASG.-....Q....IR.S.SH..S.RLKR.L..V..NA.TQANTA.RQ.TE------WKY
Crithidia: mBiP ...TQ.G.K.FQ...E-.AA..QMM.Q...V...........V...........CH.T....-A...TQN....ANG.-....Q..Q.IR.S.AH..T.RVKR.L..V..NA.TQLTTA..Q.GE------WKY
mouse: mBiP .G.TQ.E.K.CQ...E-.AG......Q.T.I.......................VH......-...R.QQ.V.QSSG.-...DD..N..KN...Y....RRK......V.MA.GIIHDT.TKM.E-----FKDQ
Homo sapiens mBiP .G.TQ.E.K.CQ...E-.AG......Q.T.I.......................VH......-..RR.QQ.V.QSSG.-...DD..N..KN...Y....RRK......V.MA.GIIHDT.TKMEE-----FKDQ
Rattus sp. mBiP .G.TQ.E.K.CQ...E-.AG......Q.T.I.......................VH......-...R.QQ.V.QSSG.-...DD..N..KN...Y....RRK......V.MA.GI.HDT.TKMEE-----FKDQ
Cricet. gris. mBiP .G.TQ.E.K.CQ...E-.AG......Q.T.I.................L.....VH......-...R.QQ.V.QSSG.-...DD..N..KN...Y....RRK......V.MA.GIIHDT.TKMEE-----FKDQ
pea: mBiP ...TQ.G.K.LQ...E-.AA...S..E...V........L..............VT......-S....QQ...RSSG.-..DD..DK..KE..LH.QR.QE..ALIDI..SADTSI..I..S.AE-----YRE.
bean: mBiP ...TQ.G.K.LQ...E-.AS...M..E...V........L..............VT......-S....QQ...RSSG.-..ED...K..KE..LH.QK.QE..TLIDI..SADTTI..I..S.GE-----YRE.
Luffa: mBiP? ....A...K.FQ...E-IAAG..A..E.N.E....S...M...............H......-..........KANS.-..ED..Q...K...AN....HRVA.LAQ...QADAL.HATR.S.TE-----Y.D.
potato: mBiP ...TQ.G.K.LQ...E-.AS......E...V......K.*.............MVT......-A.S..QQ...RSSG.-..ED..DK..RE..MH.QRI.NARHLLISGIVQSTTI..I..S.SE-----YKE.
Lycopers. per. mBiP ...TQ.G.K.LQ...E-.AS......E.E.V........M.............MVT......-A.S..QQ...RSSG.-..ED..DK..RE..MH.QK.QE..ALIDI..SADTTI..I..S.SE-----YKD.
Spinacia oler. mBiP ...TQ.G.K.LQ...E-.AS...M..E.E.Q........L..............VT......-A.N..QN...KSSG.-..EH...K..KE..IH.QK.QE..SLID...TADTTI..V..S.GE-----YRE.
Giardia: BiP .Q.TM.K........S-.V...N...N...ND.......T.......E..S....T...VE.-SS...ES...K..R....ED..N.L.KE..E......INR..A....AF.MI.SITTTQTTADKEGNIVD.
yeast: BiP ....T.M.K.......-.S...N.....E..................AL......K...T..-....SES..........TQ...D...EE.....S..ASI.AK..S..K..N.AH.LKNQVNG----D..E.
K. lactis: BiP ...KA.R.........-.V...N...N.E.SD.RA............AL......T...T..-D...SES...A.......QDD.D...EE...Y.A..A.F.AKS....TF.NF.HYVKNSVNG----E.AEI
S. pombe: BiP ...NT.L........T-L............R................EV....V.T...V..S.K..PE.LV.K.......E.D.....KE..E......IL...I....T..N.A..LKGQFD.--D.Q....
Achlya a.: BIP ....A.S...F....T-.....HM....E..................EV......Q...E..-....AE.....AE..S..Q...D...QE..EN......I.D.IDG..S..G.L.NLKNNVEE----S.TD.
Phaeodact. tr. BiP ...NV.L........S-.....HN...FE..................EV......Q...E..-....AE.....AE.....E.......RE..E.......V...ID...G....L.NLKN..D.-DEKG.ADN
Plasmodium: BiP ....A.L...F.....-L....H.....E.S.....Q....K.....TV.K....H.E.E..-....SRG.............Q..K.I.......D...NLR.K...K.N.DN.IQ.MKA.VE.--KD..AD.
P. falciparum: BiP ....A.L...F.....-L....H.....E.S.....Q....K.....TV.K....H.E.E..-....SRG.............Q..K.I.......D...NLR.K...K.K.DN.IQ.MKA.V..--KD..AD.
C. elegans: BiP ....T.....F....P-.....HQ........L..............E..V....H.T.E..-...NK........QN...P.D..A.I........D...V.DKA....W....A.NLKNQIE.--K......
E. granul.: BiP: ....T.....F....P-.....HF...............L.......E..V....R...E..-....KSN.V.NKETN.ITP......IQ.....SDQ..QV.....V..D...LA..IKNQV..--K..M...
E. multiloc.: BiP: ....T.....F....P-.....HF...............L.......E..V....R...E..-....KSN.V.NKETN..TP......IQ.....SDQ..QV.....V..D...LA..IKNQV..--K..M...
Aplysia: BiP: ....T..........S-.....H........................E..V....K.T.E..-...SK.Q.V.Q..QN...P.D....I.....Y.D....V..K.D.K......A..LKNQIG.--K....A.
Drosophila: BiP ...HT..........P-.....H................I.....S.E....A..T...E..-...NKE..V....QN..TP.D.D..IR......D....L.....S.......A..LKNQIG.--KD...A.
chicken: BiP ....T...K......P-L....H...T....................E..V....R.T.E..-...NK........QN..TP...................L...ID........A..LKNQIG.--K......
hamster: BiP ....T...K......P-L....H...T....................E..V....R.T.E..-...NK........QN..TP...................L...IDT.......A..LKNQIG.--K......
rat: BiP ....T...K......P-L....H...T....................E..V....R.T.E..-...NK........QN..TP...................L...IDT.......A..LKNQIG.--K......
pig: BiP ....T...K......P-L....H...T....................E..V....R.T.E..-...NK........QN..TP...................L..
mouse: BiP .E..-...NK........QN..TP...................L...IDT.......A..LKNQIG.--K......
human: BiP ....T...K......P-L....H...T....................E..V....R.T.E..-...NK........QN..TP...................L...IDT.......A..LKNQIG.--K......
maize: BiP: .Q.TT.S...F....S-....CR.......N..A.....T.......EV........K.E..-....SE......E.....Q...D...RE..E.......V...ID...Q..T...NMKN.VG.--KD..AD.
tobacco: BiP1 .Q.TT.S...F....S-L...CR..........A.....T.......EVD.......K.E..-....SE............Q.......RE..E.......V...ID...S..T...NMKNQIN.--KD..AD.
tobacco: BiP3 .......T.......EV........K.E..-....SE............Q.......RE..E.......V...ID...G..T...NMKNQIN.--KD..AD.
tobacco: BiP (A) .Q.TT.....F....S-L...CR..........A.....T.......EV........K.E..-AS..SE............Q.......KEA.E.......V...ID...S..T...NMRNQIN.--KD..AD.
tobacco: BiP (B) .Q.TT...S.F....S-L...CR..........A.....T.......EV........K.E..-AS..SE............Q.......KE..E.......V...ID...S..T...NMRNQIN.--KD..AD.
tobacco: BiP8 .Q.TT.....F....S-L...CR..........A.....T.......EV........K.E..-AS..SE............Q.......KE..E.......V...ID...S..T...NMRNQIN.--KD..AD.
barley: BiP .Q.TTVS...F....S-....CR.......S...A....T.......EV........K.E..-....SE......E.....Q...D...KE..E.......V...ID...Q..T...NMKN.VG.--KD..AD.
Giardia: hsc70 ...TT...RIF....P-L....N...T...G........T.K....Y.VS.D.VLT.T...LG...NSKQLS.NQNAN...Q...D...K...R..K..E.IR.GQK.......L.F.VKS..GE-----GKVQ
Achlya ambisexualis: .Y.TQ.NVCI......-CVDH.NK..E.TIS..DG-Q..E..VQ...E...........L..-K.NAKAET..S.NH....QAD.D...EE....KK..AEALR.LKLEARNALESFIYRALELAREKG
yeast: hsc7OA1 ...TT.QFP..Q...V-NC.E.T...E...KN..MM.A.E.VL.AI.EV......K.T.VE.-S...SSN...S.AV....S....K...Q..E.KAA.EAFAKKH...QR.....A.I.Q.VT.---PV.SS.
Kluyveromyces: hsc70 .H.TT..FP..Q...V-NC.E.T...E...K.V..M.A.E.VL.AI.EV......K.T.VE.-S...SAN...S.AI....S....Q...Q..E.KAA.EAFAKKH...QR....IS.VQQ.VT.---PV.SA.
yeast: SSA1 ......L...F.....-K....N.....E.S.................V.S........VE.-....S...............D..K..AE....K...E.ESQ.IASK.Q...IA..LKN.ISE-----A.D.
yeast: SSA2 ......L...F.....-K....N.....E.S.................V.S........VE.-....S...............D..K..AE....K...E.ESQ.IASK.Q...IA..LKN.ISE-----A.D.
yeast: SSA3 ......L...F....T-R....N.....E.S............D...............LE.-....S..............DD.D...SE...YRAD.EREA...Q.K.Q....AFTLKN.INE---ASFKE.
yeast: SSA4 ......L...F....T-R....N.....E.S............................VE.-....S...............D.DK..AE....KA..EQEAQ..Q.K.Q....AFTLKNSVSE---NNFKE.
Hansenula: hsp70 ......L.........-K....N.....E.S............................VE.-....SQ................D...AE...YK...E.EAA.IA.K.G....A..LKQ.ASE---KQFEE.
Cladosporium: hsp70 ......L...F.....-R...IN.M...E.S..R..............L.....M....LE.-....T...V................LA....YK...EAEAG.IQ.K.G....A..LKN.VS.---P.VEE.
Blastocladiella: hsp ......L.........-R....N.....E........PR......S..VG.D.......V..-S.NRS.....................AE...YKK..EEAAS.IQ.K.G....A.NLRN..N.---D.VA..
Entamoeba: hsc70: ......L...F...AS-..NHCN.....E..............................E..-T...K...............Q.DK..AE....KA..D.M.Q....K.K..NFC..VKN..SE----QFAD.
Trich. vagin. ....A...K......T-R.R..N...T.....................VN.D..M....Q..-S..NVK....K.......QAD.D...S....YKAQ.EEI.KKT..K.G..G.CFGVRNSVNG----E..S.
T. brucei: hsp7OA4 ......H...F....T-....CH...T...S.................L......S...EE.-....R.Q.V..........AD.....S..A.YEA...AHV??ID.K.G..N.AF.MKN.IN.---PNVA..
T. brucei: hsc70 ...SE.E...F....P-L.RH.HR..S.V.D..T..KH.E.T.T...SV..D...T.T.AEEL--.SVT.TLVVENSE..TS..VQK.IEV.Q...LT.ATALA.M..TER.TQWFDRLEAVMET---VPQPYS
T. cruzi: hsp7OA8 ......H...F.....-....CH...T.E.S....P...L........L..........EE.-....R.Q.VL........RA..... F.MKNAVN.---PNVA..
L. major: hsp70.4 ...RN.V.K......P-LVSQCQC..T.T..D...M...KAR.N....VNTD...I...VEE-SG.RKEA...Q..T......Q..S..RE........RMNS.......T..N.TF.MRA..D.---PDVQNG
L. amazonensis: A ......H...F.....-....CH...T...S.................L..........EE.-....R.Q............D.........M.YEAD..AQAD....K.G..N.A..MKN..G.---SNVS..
L. amazonensis: C ......H...F.....-....CH...T...S.................L..........EE.-....R.Q............D.........M.YEAD..AQAD....K.G..N.A..MKN..G.---SNVS..
Leishmania: hsp7OA3 ......H...F.....-....CH...T...S.................L..........EE.-....R.Q............D
L. donovani: hsc70 ......H...F.....-....CH...T...S............................EE.-....R.Q............D.........M.YEAD.RAQRD....K.G..N.A..MKN..G.---SNVS..
L. brazil.: hsp70 ......H...F.....-....CH...T...SA................L..........EE.-....R.H............D.........S.YEQA..MQRDAL..K.G..N.A..MKN.VS.---TNVS..
Theileria: .R.E..L...F.....-.I...N.....H....A.......................T.M..-S...SEHV..........Q...D...EE...YK...E..RRC..S.HK..N.C..MKN..SE---DQVKQ.
Theileria parva .R.E..L...FK....-.....N.....H....A.......................T.M..-S...SEHV..........Q...D...EE...YK...E..RKC..SKHK..N.C..MKN..SE---DQVKQ.
Plasmodium:hsp7OA ......L.........-...G.N.....Q.E.......S..................T.L..-....Q.Q............DD.D........YK...EQN.N.I....N..N.C.NVKN..Q.---.N.KT.
Plasmodium:hsp7OA1 ......L.........-L....N.....H.D.......K..................T.VE.-S...Q.H...........QD..D........YKA..EENRK.I....S..N.C.GVKSS.E.---Q.IKE.
Plasmodium:hsp7OA1* ......L.........-L....N.....H.D.......K..................T.VE.-S.A.Q.H...........QD..D........YKA..EE-RK.I....S..N.C.GVKSS.E.---Q.IKE.
Plasmodium:hsp7OA2 ......L.........-L....N.....H.D.......K..................T.VE.-S...Q.H...........QD..D........YKA..EENRK.I....S..N.C.GVKSS.E.---Q.IKE.
P. chabaudi:hsp70 ......L.........-L....N.....H.D.......K..................T.VE.-S...Q.H...........PD..D........YKA..EEN.K.I....S..N.C.GVKSS.E.---Q.IKE.
P. cynmolgi: hsp70A2 ......L.........-L....N.....H.D.......K..................T.VE.-ST..Q.H...........P...D........YKA..EEN.K.I....S..N.C.GVKSS.E.---Q.IKE.
P. falcip.: hsp70A ......L.........-L....N.....H.D.......K..................T.VE.-S...Q.H...........QD..D........YKA..EENRK.I....S..N.C.GVKSS...---Q.IKE.
Eimeria: hsc70 ......L...F.....-.....N.....H.D.......................M..T.TE.-N...S.Q...........QG..D...AE...YKA..EAN.Q....K.A..N.C..MRG.ME.---..IKD.
Euplotes eurystomus ......L.........-.....N.....N.E............................V..-....................D..K...E....KD..E.QRKKI.SKNS..H.ITTVKH..E.---.N.KT.
Cryptosporidium par. ...S..L.........-.....H.....H.D............................V..-S...SS.............*D..........YKG..EQNRLKI..K.S..N.L.NMRN.IQE---P.VKE.
Babesia microti N..T..M...F....S-..C..N.....H.S............K...........T...A..-S...TEHV........L.QQD.D...AE.A..K...E.K.RC..SK....N.C..MKN..E.---.NIKS.
Chlamy.: hsc70: ......L.........-R....N.....E..............N.I...........S.E..-T..NK........K.....D......QE...YKAD.EQL.-K...K.S..N.A.NMRN.IRE---D.VASQ
Bremia: hsp7O ......L..LF....S-..R..N.....N.D....M.......D..........F....VE.-S............RVVCRRHD.D...SE...YKS..EAN.I.I..K.G..N.S.NLRN..N.---...KDQ
Achlya: hsc70 ......L...F....S-..R..N.....S.D....M.......D...............VE.-S................T.DD.....QE...YKS..EANNV.I..K.G..N.A.NLRN..N.---...Q..
Pyrenomonas: hsp70 ......L...F....S-R....NI....E..............................S..-S...S.....................EE...YKT..E.LDKKL..K.S..N.A.NIRN.VR.---...KE.
Ascophyllum nodosum ......M...F.....-.....NQ....E.G............................E..-S...TT.......T....A....K..S..S...KD.EAA.ALI..K.G..N.AF.LRN....---..VA..
Guillardia th. hsc70 ......L........Q-.....N.....Q.D..........K............VH.Q....-A....Q............QDD.....KE..G.K...ERQRKK.D.K.S..N.A..LKN..S.---..VAES
carrot: hsp70: ......L.........-R.R........E..............N.V...........F.E..-TA.VK........N.....D...KL.KE...YKA..EEV.KK...K.A..N.A.NMRN.I..---D.IP..
petunia: hsp7O ......L.........-R....N.....E.S............T.C.............E..-T..QK..................K..QE...YKS..EEL.KK...K.A..N.A.NMRN.I..---D.INSQ
spinach: hsc70 ......L........T-R.R..N.....E.S....G.......N.C.............E..-T..QK...R..............K..QE...YKS..EEH.KK..SK.A..N.A.NMRNIV..---..I.A.
tomato: hsc70A ......L...F.....RR.R..N.....E.SV......V....T.C.............E..-T..QK..................K..QE...YKA..EEL.KK...K.S..N.A.NMRN.V..---..I.S.
tomato: hsc70B ......L........T-R.R..N.....E.S............T.C.......T.....E..-T..QK..................K..QE...YKS..EEH.KK...K.A..N.A.NMRN.I..---..IAS.
Arabidopsis: 1 ......L.........-R....N.....E.S............T.C.............E..-T..QK..V...........D...K..QE...YKS..EEH.KK...K.A..N.A.NMRN.IQ.---..I.E.
soybean: hsp70 ......L...F.....-R....N.....E.............VN.C.............E..-TA.VK..................K..K...RYKA..EEV.KK...K.S..N.A.NMRNYI..---..I...
tobacco: hsp70 ......L........P-.....N.....E.K............N.C.............E..-TA.VK...................L.QE..RYKA..ESM.KK...K.S..N.A.NMRN.VR.---..FS..
maize: hsp7OA1 ......L.........-R....N.....E.S............T......V.N......E..-T..QK..................K..QE...YKA..EEV.KK.D.K.A..N.A.NMRN.I..---D.IAS.
lily: hsp70 ...S..L........T-RS...N.....E.L..S..A......T...............E..-T.RQK..................K..KE..RYKS..E.H.KQ.A.K.A..T.I.NMRNAVN.---.RITS.
Lupinus p.: .KE...L...F....T-R.R..N.....E.S.......D....T.C......WC.....E..-T..QK...............D..K..QEV..YKA..EEV.KK.DTK.A..N.A..MRN.IT.---D.ITS.
sea urchin: hsp70 ......S...F.....-.....NR..Q.E.S........R.K...S........MHVT...E-SS.RS........SD....DD.D..I....R.KA..DAQR..IN.K.Q..G.AFNLKSAVD.----AAAQS
sea urchin: hsp70-4 ......S...F.....-.....NR..Q.E.S........R.K...S........MH.T..DE-SS.RS........SD....DD.D..I....R.K...DAQR..IN.K.Q..G.AFNLKSAVD.---AAAQS.
Trich. britovi mBiP? ......L.........-.....N....FEL.............................V..-S..RQ...............D.D...RE.DQYKQ..E.QRD.IQ.K.G....AFNVKS.IE.---...KD.
Dirofilaria: hsp70 ......L.........-.....N.....E.S............................Q..-S...Q..............D......QE...YKAD.EAQ.D.IA.K.A....AFNMKQ.IE.---...KD.
B. malayi: ......L.........-L....N.....E.S............................Q..-S...Q..............D......QE...YKAD.EAQ.D.IA.K.A....AFNMKQ.IE.---...KD.
Brugia pahangi: ......L.........-L....N.....E.S..........E.................Q..-S...Q..............D......QE...YKAD.EAQ.D.IA.K.A....AFNMKQ.IE.---...KD.
O. volvulus: ......L.........-.....N.....E.S............................Q..-S...Q..............D......QE...YKAD.EAQ.D.IA.K.A....AFNMKQ.IE.---...KD.
C. elegans: hsp7OA ......L.........-.....N.....E.S............................T..-S...AKQ.......D.F..DD......E...YKAD.EAQ.D.IG.K.G....AFNLKQ.IE.---...KD.
Dros.: hsc70-4 ......L.........-.....N.....E.S..........................T.LER-S.N.................D......E...YRN..E.Q..TIA.K.G....CFNMKA..DE---DN.KT.
Dros.: hsc70-1 ......L...F.....-..R..NS....E.SA..........V..............T.LE.-S.....R.............D.........AYRQA.EQQRD.IN.K.Q....CFQLRS..D.---.H.SSR
mosquito: hsp70-1 ......S...F.....-.....N...Q...S.................L........A....-SS...KN...K.......QAD.D...SE....R...E.QR.AIA...Q..A.CFNLKQS.DG----EGSS.
mosquito: hsp70-2 ......S...F.....-.....N...Q...S.................L........A..E.-S....KN...
mosquito: hsp70-3 ......S...F.....-.....N...Q...S.................L......N.A..E.-S....KN...K.......QAD.D...SE....R...E.QR..IS...Q..A.CFNLKQS.DG----EGAS.
Dros.: hsp7OA2 ......S.........-.....NA..T...S.................L...........EM-S...AKN...K.......QA..D....E...Y.D..E..RQ..TS..A...H.LNVKQAVEQ----APA..
Dros.: hsp7OA4 ......S.........-.....NA..T...S.................L...........EM-S...AKN...K.......QA..D....E...Y.D..E.HRQ.ITS..A.....FNVKQSVEQ----APA..
Schistosoma: ......L...F.....-L....N.....E.S........T...................V..-....Q.....................A..D.YKA..E.QRD..S.K.S......TMKQQVEG----E.KE.
S. japonicum: hsp70 ..T-.....N.....E.S............................V..-A.....................D..I.E.DRYKS..E.Q.N.IC.K.S.......MKQSVEG---DEMKD.
Hydra m.: hsp70 ......L...F....S-..AH.N.....E.S...L.............V..........L..-S...................D.....QE...YKAD.ELQRDK.Q.K.S....C.NMKQ.VE.---..VK..
salamander: hsp70 ......L.........-.....S.....E.S............................V..-SS..Q.....................QEA.RYKAD.EAQR.K.S.K.T...IAFNMKS.VEG---DN.KD.
rat: hsc7OA ......L.........-.....N.....E..............................V..-S...................D.....QE...YKA..E.QRDK.SSK.S....AFNMKA.VE.---...Q..
mouse: hsc7OA ......L.........-.....N.....E..............................V..-S...................D.....QE...YKA..E.QRDK.SSK.S....AFNMKA.VE.---...Q..
hamster: hsc7OA ......L.........-.....N.....E..............................V..-S...................D.....QE...YKA..E.QRDK.SSK.S....AFNMKA.VE.---...Q..
cow: hsc70 ......L.........-.....N.....E..............................V..-S...................D.....QE...YKA..E.QRDK.SSK.S.K..AFNMKA.VE.---...Q..
human: hsc7OA ......L.........-.....N.....E..............................V..-S...................D.....QE...YKA..E.QRDK.SSK.S....AFNMKA.VE.---...Q..
mouse: pseudo1 ......L.........-.....N.....E. ...AFNMKA.VE.---...Q..
human: pseudo1 ......L..L....HT-....DN..D..E...................TNS........V..-S.R.K..........H....D..Y..QE...YKA.EQ.QR.SGASKDS.D.CESNMKA....---...P..
chicken: ...SS.LV........-.....N....................................V..-S..................DD.D...EE...YKA..EANRD..G.K.S....T.NMKQ.VE.---...K..
mouse: hsc7OB ...SS.LV........-.....N..................................T.A..-S..................DD.D...QE..RYKS..EANRD..A.K.AV...T.NIKQ.VE.---...R..
mouse: hsc7OB* ...SS.LV........-.....N................................? .?..-SS.................DD.D...QE..?YKS..EANRD.LA.K.AV...T.NIKQ.VE.---...R..
human: hsc70B ...SS.LV........-.....N..................................T.A..-S..................DD.D...QE..RYKS..EANRD..A.K.A....T.NIKQ.V..---...R..
cow: hsp70 ...SS.LV........-.....N..................................T.A..-S..............C...DD.D...QE..RYKS..EANRD..A.K.AV...TYNIKQ.VE.---...R..
Xenopus: hsp7OA1 ......L...F.....-.....N.....E.S............................VE.-SS..Q...............D..K..QE...YKAD.DAQR...D.K.A....AFNLKSMVE.---.NVK..
mouse: hsc70C ......L.........-..R..N...R..............................T.M..-S...A.....................QE..RYKA..EGQR.KIA.K......AFNMKSAVG.---.G.KD.
human: hsp70B ......F.........-.....N...R.E.S........................S.T.T.R-S...A................V....HE..QYKA..EAQRD..A.K.S..AH.FHVKGS.QE---.S.RD.
pig: hsp70 N.....L.........-..R..N...R.E.SR...T...................S.T.T.R-S..RA..M.L.S.........V....RE.ASHKV..EAQRD..A.K.S..A..FHVKGS.HE---.S.RD.
pig: hsp70com: ......L.........-..R..N...R.E.S..........................T.T..-S...A.....................QE...YKA..EIQR...G.K.A....AFNMKSVVE.---.G.K..
mouse: hsp7OA1 ......L.........-..R..N...R.E.S..........................T.T..-S...A.....................QE..RYKA..EVQRD..A.K.A....AFNMKSAVE.---.G.K..
mouse: hsp70A2 ......L.........-..R..N...R.E.S..........................T.T..-S...A.....................QE..RYKA..EVQRD..A.K.A....AFNMKSAVE.---.G.K..
human: hsp7OA1 ......L.........-.....N...R.E.S......-...................T.T..-S...A.....................QE...YKA..EVQR...S.K.A....AFNMKSAVE.---.G.K..
human: hsp7OA2 ......L.........-.....N...R.E.S..........................T.T..-S...A.....................QE...YKA..EVQR...S.K.A....ASNMKSAVE.---.G.K..
human: hsp7OA** .................GA..QE...YKA.?EVQR?.ISS..A?...AFNMKSAVE.---.G.K..
CONSENSUS DNQPGVTIQVYEGERA-MTKDNKLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVSAKDK-GTGKENKITITNDKGRLSKEEIERMVNDAEKFAEEDKKRKERVEARNELESYVYSXKNTVKD---EKLGGK
*NO1
*NO2 56O 575 59O 6O5 62O 635
*NO3 | | | | |
*2nd CONSENSUS LSEADKEKIEDAIXEALEWLDSNQ-----TAEKEEFKAKXKELEXVCQPIISKLYQGA------------------------------------------XQA-XXGGXXSGPTXEEVD-----------------
E. coli: hsc70 ..A.ERQV.D..A-AH.SEVAQGD-------DVDAIEKAI.NVDKQT.DFAARRMDQSVRRAL--KGHSVDEV
H. influenzae: hsc70 ..TDEFHH..TVL-KQ.MDVKQGS-------DRDAIAQGI.A.DTAT.EFAARRMNASINKALTGKNLSDIENP
Buchnera aph. hsc70 ITSEELK..KSHQ-IK.QKSIDED-------DFFSM.LNL.K..E.SKNFL.LRLKKNMD-SFSIKNLSDEIT
B. megaterium: VE..EVT.ANE.K-D..KAAIEKN-------DL..I...KD..QEIV.ALTV...EQ. QQAQQ AGEQGAQ NDDVVDAE FEEVNDDKK
B.subtilis: dnaK VE..EVT.ANE.K-D..KAAIEKN-------DL..I...KD..QEIV.ALTV...EQ. QQAQQ AGEQGAQ NDDVVDAE FEEVNDDKK
Bacillus stearo. VD..EVK.ARE.K-D..KAALEKN-------DLD.IRKKKEA.QEAV.QLSI...EQ. AKQAQ NQQAGADGATK KDDNIVDAE FEEVKDDK
Staph. aureus: IG.E..KSA.EKK-D..KTALEG.-------DI.DI.S.KE...K.I.ELSA.V.EQ. AQQQQQAQGANAGQNN DSTVEDAE FNEVKDDDKK
Streptococcus pyog. GFDTERDAAQS.L-DE.KAAQESG-------NLDDM...LEA.NEKA.ALAV.M.EQ. AAAQQAAQGAEGAQAN DSANNDDVVDGE FTEK
Streptococcus mutans GFDTERDAAQA.L-DD.KKAQESG-------NLDDM...LEA.NEKA.ALAM...EQ. RAAQQAQAGQEGA
Streptococcus agal. GFDTERDAAQS.L-DE.KKAQESG-------NLDDM...LEA.NEKA.ALAV...EQ. AAAQQAAQGAEGAQSA DSSSKGDDVVDGE FTEK
Streptococcus pneu. GFD.ERDAAQA.L-DD.KKAQEDN-------NLDDM...LEA.NEKA.GLAV...EQ. AAAQQAQEGAEGAQA TGNAGDDVVDGE FTEK
L. lactis: dnaK VE..EVK.A...K-.E.KKALEGE-------DIDDI...SEA.SEIA.NLAV...EQ. NAAQG EASEATDAQEGPKDANT FDGD FEESK
Erysipelothrix: dnaK VDD.Q..EVTKLR-DE.QAAMD.N-------DF.TL.E.LDQ..QAA.AMSQAM.EQQ AGQA EVDASSS DETVVDAE FEEKN
Mycoplasma pneum. FPKEE...LDKIT-GGIDAAIKAN-------DYTKL..EIENFKKWREEMAK.YNPNG DQGQPAQ
Mycoplasma gen. FPKEE...L.KLT-GNIDAAIKAN-------DYAKL.VEIENFKKWREEMAK.YNPTG EQGPQAK
Mycobacterium tuber. VP.DTLN.VDA.V-AEA.GGTWRI-------GYFGHQVGDGEAGPGVAGSGASDLRSSSGCVTGHWRCPPRRRAGRCPPRLG
M. tuberculosis: VP.DT----------------WRI-------GYFGHQVGDGEAGPGVAGSGASDLRSSSGCVTGHWRCPPR-AAGRCPPRLGM
M. leprae: dnaK VP.DTLN.V.A.V-AEAKTALGGT-------DISAI.SAMEK.GQDS.ALGQAI.EAT QA ASKVGGEAS APGGSNS TDDVVDAE VVDDERESK
M.paratuberculosis: VP.ETLS.VDA..-ADAKTALGGT-------DITAI.SAMEK.GQES.ALGQAI.EAT QA ESAQAGGPD GAAAGGGSGS ADDVVDAE VVDDVRESK
Streptomyces: dnaK VPGEV.TEV.S.V-AE.KEKLKGE-------DTA.IRTATEKVAA.S.KLGQAM.AD. QA AQAAGGEAPGADAGAEGKGA DDDVVDAE IVDDERKDGAA
S. griseus: dnaK VPADV.TEV.T.V-GE.KEKLKGE-------DSA.IRTATEKVRGRLPR.WARRCTAN AQ AEGAAPG ADA PGDAQAKA DDDVVDAE IVDDEKDTKGGAA
Clostridium: dnaK V.AEE.SE..AK.--EVKKVKDGD-------DIQAI.KAMED.TQAFYKVSE...QQN GGAQGQGFDPIIMGGANAGAGATNNN DDNVVDAD FEVQVDK
Clostridium aceto.: V.AE..SN..AKK-...SKVKDGD-------DI.AI.KATED.TQA
Methanosarcina: .ATE.QKSKVN.AI.D.KKALEGK-------DA.DI...TEA.QESVY..STAM.QKA QQAQQAAGGEG GAAGTDARGP DETVVDAD YEVVDDEKRK
Halobacterium: EIDE.LQSDIE.KI.DV.EVLEDE-----DAT..DYE.VTET.SEEL.E.GKQM.QDQ AQQAQAVPRALVRVARPAPGALPDRAAQQAAAEQG AEEYVDAD FEDVEESDEDE
Halobact. cut.: VD.DLEADVN...DDVQAV..EDE P.IDALETATE..SDTL.E.GKQA.QQQ QDM PRPARPAALAAWVAWAA WPTARRGGRRRRD DEEYVDAD FEDVDEE
Thermoplasma a.: VDKET..RLTNEVKDLRKAIEEKN--------T.NV.TLMDK.SKDI.EVGA.M.QQA SANTQQSAQSNS
Synechocystis: dnaK1 VR.EL.SSGKQKK-DQ.AIALRTP-NTPVEKLQTLVEEFRQIILLIGTEVYQQGKGAASNEFANFVQGTSSGTSQMIESLETNLNPIQASSGITMTPGTMINGTVNPGGMDYVETAPVYGLDDGEEFD*
Synechococcus: dnaK1 .TAEQQ.PL.ASL-N..ASGLESV---SNE..LNQLRQQLEA.KQQLYA.GAAA.RQDGSVTTIPVQPTFADLIGDNDNGSNETVAIERN DDDATVTAD YEAIE
Therm. aquaticus PEARARLEAAIGK-AKELVERDAP--------DP.L..ATE..LKAVEEYEKGAQAASGKG PDDVIDAD YKPAD
Deinococcus prot.: PKTPRTDSSRGRR-SRAGRGQRRR------QRIA.VQGRLE.ELRNFMTQN.AAAAQEGAGQPGAAQ DQDDVIDAD FKPADDNK
Thermomicrobium ros: SELKLELENKMQA-VREIVERDPM----N..RLRGLRRAGADPLPGRRPHVRAGPAPTASTGPDGGRTSGAPGEETVEGEYREV
Synechocystis: dnaK2 VPA...I.A.GL.-KD.KEAVAQE-------DDAKIQTVMP..QQ.LYS.G.NM..QAGAEAGVGAPGAGPEAGTSSGG GDDVIDAE FSEPEK
Synechococcus: dnaK2 I.ADE.S.V.GF.-QE.KDALAAE-------DYDKI.SIIEQ.QQALYAAG.SV..QASAEASANAQAGPSSSSSSSSG DDDVIDAE FSESK
Synechocystis: dnaK3 FGS.FTVSYRRQV-D..CSEILDS---LEKDDERRLDRAQAD.QD.LYELNREVRLQYDDKE-EGFFEAIKKTFTGDFDDDDDYYNRRPAPRDDYRGGNDYGRYDDYNYNAPSRREAPMPRAGAGR*
Synechococcus: dnaK3 FGLYFAESKRRR.-.STIRELKDY---LERQDDRGLDLALA..QDALFDLNQETAARLRDEEGEGFFEPLKQTFASLRGDGNRDFERSWDDRGGDRWDADPWDRSRR STPSYGYDDRRSPVSDPYRGER*
Anabaena variabilis FGMQFARNRRQR.-DNISRELKES---LKENDDRGIDQAYAD.QDALYELNREVR.YYAEDEDDDLFATIKDIFVGDKDKERDLPRDSYRERDSYNNRDYGRDYGRDYGRDSRPSYDSNRPPRKSPRPSYQDN*
Aphanothece halo. IPQKV.--ATANK-KKE.LMAILE---DPNV.LGVIQTRLEDYRQAVLSMG.EV.SQGSSKSSARDYETVGEEEMTRETGNSQESNAAFSTQPEGTVNNDNLELEAVFSTYNDHTQGSDVATSSHDEIDLG*
Porphyra: cBiP: ID.EL.NR.TNL.-SE.RSNLEKE-------.LDSIE.NSEK.QNALME.GKNATSAEKDTQN ASN DDTVIDTD FSEAK
Chlamy. rein. cBiP VPADI.A.V.AKL-GE.KAALPAD-------DA.AT..AMNA.QQEVMAMGQAM.SQAGAAPGGAPGAEPGAGAGAGGAPGGKK DDDVIDAE FTDKK
pavlova: cBiP .VNENQSL.KESK-.TV.M.KE.I----KKEDYDKI.ENL.K.QEKLME.GQKAYAKKEPLKDEDSNK AGS QDDFIDAD FTESK
Odontella sin. cBiP IP.EKQQNVQKL.-.NIRQDTQTD-------NF.SL.LLVE..KMAMKDMVEAKPFVDQTDTDPMSNLNDL
Cryptomonas: cBiP: IDDTN.N..SSM.-SE.RNAIN.E-------NYD.MRDLNSK.QTALMDLGKSV.EKTSKEQTSTSSP TNS NDSVIDAD FSETK
Spinach: cBiP VPVPV...V.AKL-GE.KDAINGG-------.TQAI.DAMAA.NQEVMQLGQS..NQPGAGGEPGAAQAQHQEQSSARQIQRAKDPKEMLLMLTSQTASELVEEVVSKMH
Pisum sativum cBiP VPAPV...V.AKL-GE.KEAITGG-------STQTI.DALAA.NQEVMQLGQS..NQPGAAGQAGPTPPGSESGPSESSGKEGP EGDVIDAD FTDSK
Cucumis sativ. cBiP VPGPV...V.SKL-GE.KEAI.GG-------ST.AI.EAMAA.NQEVMQLGQS..NQPGAGAAPGP-GASSESGPSESTGK-GP EGDVIDAD FSDSK
Cucumis sativ. mBiP VPGPVKEKVESKL-GELKEAISGG-------STEAIKEAMAALNQEVMQLGQSLYNQPGAGAAPGPGASSESGPSESTGKGP EGDVIDAD FSDSK
Citrullus lan. cBiP VPGPV...V.SKL-GE.KEAI.GG-------ST.AI.EAMAA.NQEVMQLGQS..NQP--GAAPGA-GAGSESGPSESTGK-GP EGDVIDAD FTDSK
Aquifex aeolicus VPADV.SEA.KV.-.EAKKTIETA------T.I.QV.QVTEKVLQ.SSKMGTT..GEAGKQAGGGEKK DEGGEGEV EAKPVD
C. pneumoniae: IP.TLVKE..ER.-.NVRNALKDD------API.KI.EVTED.SKHM.K.GESMQSQSASAAASSAANAKGGPNINTEDLKKHSFST KPP SNNGSSE DHIEEADV EIIDNDDK/S
Chlamydia: dnaK IPAELVKE..EHL-.KVRQAIKED------ASTTAI..ASD..STHM.K.GEAMQAQSASAAASSAANAQGGPNINSEDLKKHSFST RPP AGGSASS TDNIEDAD VEIVDKPE
Chlamydia pneumoniae IP.TLVKE..ER.-.NVRNALKDD------.PI.KI.EVTED.SKHMQK.GESMQSQSASAAASSAANAKGGPNINTEDLKKHSFST KPP SNNGSSE DHIEEADV EIIDNDDK
Borrelia: dnaK I.SE...A..SK.-KE.KESLEKE-------DISLI.SRTE..QKASYK.AEMM.KDSSQQNANSQQENGPQSNTSEEGKEA DYEVVDED KK
Leptospira interrog. IGANE.QLAT.E.-KRAREAIESN-------D.ARIESAKASISKIATD.A..V.SQSAPGAEQAAAGGPNGAGSNDQGNSTNN GEKVVDAD YTVVDDEKK
Helicobacter pylori: .N.N.ANE.QN..-N..KDCIKTI------TLLKRIED.T.A.KGSKIRRNGEKNNAEQPKKKDDDVIDAEVE*KRF*NFFQASGFNQNPLAFLIFINGILITKSKNSVFNPKIKT
Myxococcus xanthus ...K.REE.KADL-.R.KEVLNTS-------DAAVL.ESFQR..GSAYR.ADAI.T.QAS
Caulobacter: dnaK VGA.E.TA..TG.-TE.KTALEGE-------DV.AIQ..TQA.IQASMKLGEAM.AAQQGSAE GGDAKA DDGVVDAE FEEVDDNKPAA
Rhizobium meliloti: V..T.RKA.....-A..KSRVEVS-----EPDAEDIKAKTNT.MEVSMKLGQAIYEAQQTDAAHAMPPPDAKRS GDDVVDAD YEEVKDEDDRKRSA
Bradyrhizobium japo. VA.TERRA....V-SD.KEALKGD-------DA.AI...TQT.AQASMKLGEAM.KQQAEADAKKDAA KDDVVDAE FTEVDDDKNNKKSA
Rhodoba. capsulatus: VDGSTV.M..LAC-N...ESLKSE-------DPGKIKGAVQN.TDAAMKLGEAIYKAQASEAGPASDDEDGPRSV DDDIVDAD FEDMGENKRK
Agrobacter. tum.: V..T.RKA.....-AS.KTAVEAA-----EPDADDIQAKTQTLMEVSMKLGQAIYEAQQAEAGDASAEG KDDVVDAD YEEIKDDKKSA
Brucella: dnaK V.AD..KA.....-A..KTSLEGE-------DA.DI...TQA.AE.SMKLGQAM.EAAQAAEGAGAEGGEQASSS KDDVVDAD YEEIDDNKKSS
Rhodopseudomonas sp. ID.GERRS....LSDLR.A.KGDD--------A.AI.T.SNT.AQASMKLGEAM.KQ.EAGGAAQQAG KDDVVDAE FTEVDDDKKKSA
Rhizobium legum.: V....RTA.SD..AALKTASEASE------PDADDI...TQT.ME.SMKLGQAI.EAQQAESGAAGMPPPKA GDNVVDAD YEEIKDDDRKKSA
Pseudomonas cepacia: LDAGE..AL.ASL-KS..EVLKDT-----S.D.AAID..VE..GK.S.KLGE.M.ADMQAQQAGAAGAAGAAEGAAHAGGAQQA ADDVVDAE FKEVKKD
Ralstonia eutropha ARSWREG.DRSRD-QGPGRRRPRV-------N.A.ID..VNA.SEAS.KLGE.V.ADMQAQAGEGAAAGAGAXRFSSRPSRRRQRVGCRVQGSERQEVIGDGGWRRG
Nitrosomonas europ. .EGDE.SR..A.L-KEA.EALKSG-------D.QTID..TQA.TEASHKLAE.M.AQEQAQAGQQAGAGTASDQSQDKPV EGEVVDAE FEEVKDKK
Franc. tularensis: VTA.E.....E.C-KE..AATKGD-------D.QAIE..T.A..EAFA..AQ.A.AEQAQAAGAQGGAKAEEPKK EEDVVDAD FEDVEDDKK
Legionella pneumo. ...DE.RG..T..-SE.KEAVQGT-------D.ARIED.L.V.TDASAKMAERI.AKKSSEGQAAQGQTQSQESTKPA EEGVVDAE FEEVKEEDKK
Haemoph. actinomyc. VPA..RGT..S.L-SD..KAAKGE-------D.AVIE..I.A.AE.A.KLAQTAQPHGDPQQAQSNSKP NDDVVDAE FEEVKDNK
Haemoph. influenzae: ..V.......A.V-AE..TAAKGE-------D.A.IE..IEAVIKASE.LMQAVQAKAQQAGGEQPQQSSAK DDGVVDAE FEEVKDNK
Haemoph. ducreyi: .TAE..A...T.L-AE..TAAKGE-------D.A.IE..IEAVIKASE.LMQAAQAKAQTNQAGEQQSSAK DDSVVDAE FEEVKENK
Pasteurella haem. .NAD..A...A..S.LETAAKGED--------.A.IE..IEA.IKASE.LMLAAQAK.QGGEQPQQSQK DDGVVDAE FEEVKDNK
Vibrio cholerae .PADE.A...T..N.LETAKKGED--------.A.ID..VQA.MAAAQKLMEIAQ.Q.QAQGANAGQSSAK EDDVVDAE FEEVNDDKK
Buchnera sp. IE.ES.KD.QL.LNKLENALKGED--------.SDIEKNIQN.LKISSKLTEINQKKSEKDQKDNNMSANKK DENVVDAE FEEIKDPKK
Salmonella typh.: .PAD..TA..S.L-N...TALKGE-------D.AAIE..MQE.AQ.S.KLMEIAQQQHAQQQAGSADASANNAK DDDVVDAE FEEVKDKK
E. coli: dnaK .PAD..TA..S.L-T...TALEGE-------D.AAIE..MQ..AQ.S.KLMEIAQQQHAQQQTAGADASANNAK DDDVVDAE FEEVKDKK
E. coli B: dnaK .PAD..TA..S.L-T...TALKGE-------D.AAIE..MQ..AQ.S.KLMEIAQQQHAQQQTAGADASANNAK DDDVVDAE FEEVKDKK
yeast: mBiP VDK.EAQ.VR.Q.-TS.KEWVARV-QGGEEVNA..L.T.TE..QTSSMKLFEQ..K NDSNNNNNNNGNNAES DETKQ
yeast: Yel030p .DKT.SQRLK.Q.-SSLRELVSRS-QGDEVNDDDVG-TKIDNLRTSSMKLFEQLYK NSDNPETKN GRENK
Trich. vagin. hBiP WPADLLKRIRDSVKDLKDAIAGNDKRRSKRSMMLLRLHQWKSTTLSVITRIRNKVINL
Trich. vagin. mBiP LPADLLKRIRDSVKDLKDAIAGNDEKKIEEKYDALKAASMEIYNAISDNNTNKNKK
Nosema locust. mBiP DELRRMVHSDQFDPEVAERKMEEVRARL
Vairimorpha n. mBiP EYLKQEGFDIEEVKNFIKSKL
Eimeria tenella mBiP ISAEDKTDLESRIQELRSALVEGELETIRSRVKALQELSWKVSQQAYSQS NNTSADGDSSSTSSGDSSSKP
S. pombe: mBiP .DQQAV.DLRSK.-TD.RETVAKV----------NAGDEGITS.DMKKK.DEIQQLSLKVFESVYK NQNQGNESSGDNSAP EGDKK
Trypan. cruzi mBiP SDAEKENVRTLLRACRKSMENPNVTKDELSAATDKLQKAVMECGRTEYQQAAAG NSSSSSGNTDSSQGEQQQQG DQQKQ
T. cruzi: mBiP V.D.E..NVRTLL-R.CRKSME.P------------NVTKD..SAATDYQQKAVMECGRTEYQQAAAG NSSSSSGNTDSSQGEQQQQG DQQKQ
Dros.: mBiP .PAEEC..LKKE.-AD.RT.LA.KE----..DL..VRKATSS.----.QSSL..FELAYKKMSAERET NAGAGSSDSSSSSDTSASQEG REELN
Crithidia: mBiP V.D.E..NVKTLV-ADVRKVME.P------------NVSKDD.SGATDKLQKAVMECGRTEYQQAAAA NSGSSSGNS GEQQQQQQQ GEQQQQQQ QGEEKK
Homo sapiens mBiP .PADECN.LKEE.-SKMRE.LARK--------DS.TGENIRQAASSL.QASL..FEMAYKKMA SEREGSGSSGTGEQKED QKEEKQ
Rattus sp. mBiP .PADECN.LKEE.-SKMRE.LARK--------DS.TGENIRQAASSL.QASL..FEMAYKKMA SEREGSGSSSTGEQKED QKEEKQ
Cricet. gris. mBiP .PADECN.LKEE.-SKMRE.LARK--------DS.TGENIRQAASSL.QASL..FEMAYKKMA SEREGSGSSGTGEQKED QKEEKQ
pea: mBiP IPAEVAKE....V-SD.RTAMAGE-------NADDI...LDAANKAVSK.GQHM SGGSSGGPSEGGSQG GEQAPEAE YEEVKK
bean: mBiP IPSETAKE....V-SD.RKAM.GD-------NVD.I.S.LDAANKAVSK.GEHM SGGSSGGSSAGGSQGG GDQAPEAE YEEVKK
potato: mBiP VPKEVVTE..T..-SDLRAAMGTE-------NIDDIKAKLDAANKAVSKIGEHMAGG SSGGASGGGGAQG GDQPPEAE YEEVKK
Luffa: mBiP? EAS-E.ES..A..-K...DTLKDG-------D.AAID..VEA.STAS.KLGE.M.ADMQAQQAAGQQQ AADNAKPV DDNVVEAD FKEVKRDQ
Lycopers. per. mBiP VPKEVVTEIETAI-SDLRAAMGTE-------NIDDIKPKLDAANKAVSKIGEHMA GGSSGGASGGGGAQGGDQPPEAE YEEVKK
Spinacia oler. mBiP IPTEIAKEIEDAV-SDLRSAMQND-------NLEEIKAKTDAANKAVSKIGEHMS GGQGGGSSSSGGADQTPEAE YEEAKK
Giardia: BiP I.SD.L..VKE.VK..QD..RD.T-----D.S...IEEEKSKF.K.V...LGENF GRSASAGSSGPEYD YAEKDEL
yeast: BiP .E.E...TLL..ANDV.....D.FE----..IA.D.DE.FES.SK.AY..T.... GGADGSGAADY DDEDEDDDGD YFEHDEL
K. lactis: BiP MD.D...TVL.NVN.S....ED.SD----V..A.D.EE.MASFKESVE..LA.AS ASQGSTSGE GFEDEDDDD YFD-DEL
S. pombe: BiP VDPE..QAVL..VEDVA...EIHGE----D.S....EDQRQK.DA.VH..TQ...SE.. GDADEEDDD YFDDEADEL
Achlya a.: BIP IEDER.DT.LK.VQ..MD...E..-----E.D..D.E.QQ.AV.KAVN..M..V..S SGAP GADDEEDD ED HDEL
Phaeodact. tr. BiP ..AE...ELQ.M.D.T.D.M.G.P-----E.D..DYDE.L..V.Q.AN..MRNV.S.G AGGGAEDD ADFADDEL
Plasmodium: BiP IEKE..NT.LS.VKD.ED..NN-S-----N.DS.AL.Q.L.D..A......V...GQ PGGPS PQPSG DEDVD SDEL
C. elegans: BiP LDDKDEL
E. granul.: BiP: ..DDEIKT....AD..IK.MEN.P-----Q..TSDY.KQKAN..S.V...V....E.. APPPPTESTPKEEL
E. multiloc.: BiP: ..DDEIKT....AD..IK.MEN.P-----Q..TSDY.KQKAN..S.V...V....E.. APPTESTPKEEL
Aplysia: BiP: ..DE.....TE.VD..IK..E..A-----E..S.A.NE.KT...GIV...MT...EQSGGAPPPSG EEESEEAEKDEL
Drosophila: BiP ..DDE.T.L.S..D.SIK..EQ.P-----D.DP..Y.KQK.D..AIV..V.A...... GGAPPPEGG DDADLKDEL
chicken: BiP ..SE...T..K.VE.K....E.H.-----D.DI.D..S.K....E.V...V....-.S GGPP ..G..AA-EKDEL
hamster: BiP ..SE...TM.K.VE.KI...E.H.-----D.DI.D....K....EIV........-.S AGPP ..G..DTSEKDEL
rat: BiP ..PE...TM.K.VE.KI...E.H.-----D.DI.D....K....EIV........-.S GGPP ..G..DTSEKDEL
mouse: BiP ..SD...TM.K.VE.KI...E.H.-----D.DI.D....K....EIV........-.S GGPP ..G..DTSEKDEL
human: BiP ..SE...TM.K.VE.KI...E.H.-----D.DI.D....K....EIV........-.S AGPP ..G..DTAEKDEL
maize: BiP: .EAEE...V.E.LK.......D..-----S....DYEE.L..V.A..N..V.AV.. RSGGAPGGDADGGV DDDHDELYMVVDGGGDVECREPSQIL
tobacco: BiP1 .ESDE.....T.TK.......D..-----S....DYEE.L..V.AI.N...TAV.. RSGGAPGGGSS EEEEDGHDEL
tobacco: BiP3 .ESDE.....T.VK.......D..-----S....DYEE.L..V.AV.N...TAV.. RSGGASGGSSSS EEDGHDE
tobacco: BiP (A) .ESDE.....T.TK.......D..-----S....DYEE.L..V.A..N...TAV.. KSGGAPGGESGAS- EDDDHDEL
tobacco: BiP (B) .ESDE.....T.TK.......D..-----S....DYDE.L..V.AV.N...TAV..RSGGAPGGASEESN EDDDSHDEL
tobacco: BiP8 .ESDE.....T.TK.......D..-----S....DYEE.L..V.AV.N...TAV..KSGGAPGGESGAS - EDDDHDEL
barley: BiP .ESEE...M.GSWPNNN
Giardia: hsc70 I.DD..K...T.VN.CAT..EN.K-----VGD..Q.E..K....AIVH.VMAAGMGAA GMPP GGFP GGMPP GGFP GGFP GGMP GGAA P..APA..SVDDL.
Achlya ambisexualis: DAQIESAINETRDWLEDHE-----DATLRELEEKKRVLERLVR
Kluyveromyces: hsc70 IKRNA.A.V.A.LAD.FST.QIEDA----S.DDLKAAEVGLKRAVTKAMSTR
yeast: hsc7OA1 .KRGS.S...A.LSD..AA.QIEDP----S.DELRKAEVGLKRAVTKAMSSR
yeast: SSA1 .EQ...DTVTKKAE.TIS.....T-----..S....DD.L...QDIAN..M.....-. GGAP GGAA GGAP GGFP GGAP PAPEAE...V....
yeast: SSA1* L...QDI?N..M.....-. GGAL GGAA GGA? GGF? GGAP PAPEAE...V....
yeast: SSA2 .EQ...DAVTKKAE.TIA.....T-----..T....DDQL...QE.AN..M.....-. GGAPEGA AP GGFP GGAP PAPEAE...V....
yeast: SSA2* .EQT..DAVTKKAE.TIA.....T-----..T....DDQL...QE.AN..M... -. GGAPEGG QAP GGFP GGAP PAPEAV...V..
yeast: SSA3 VG.D.AKRL.T.SQ.TID...AS.-----A.STD.Y.DRQ....GIAN..MT.F.GAG AGAGPGAGESGGFPGS
yeast: SSA4 VG.E.AR.L.A.AQD.IN...AS.-----A.ST..Y.ERQ....G.AN..M..F.-..AGGAP GAGP VPGAGAG PT.APDN...V....
Hansenula: hsp70 VDASKRESLNK..E.TIS...N..-----S.TTD.YED.R....GIANDALKDL.AA GGVP GGAAP GGFP GAG GAAP.ADQ..SV....
Cladosporium: hsp70 ..AE...TLTG..DKTVA.I.E..-----..T...YE.EQ.Q..S.AN.VMM.I.GAE GGAP GGMP GQGAG.PPP.AGDD...V....
Blastocladiella: hsp MDA....TLNK..D.TIS...G..-----EGA.D.YEH.Q....G.AN..MT...SA. GGAP GGMP GGFD PSGAP-PPAADTT...I....
Entamoeba: hsc70: IAAE..TT..NIVK.T.D.I.N..-----N.ST..YDN.M..V.GKV...FT....Q. GAA GGMP GGMP NGFP GAGAA PQGNA QPKSSGKGPTIEEVD
Trich. vagin. IDA...DA.LKIVNDT.A.IE...-----D.SVSDYENKQK.V.GQLM.LMQKA..S. AGGA GGMP GGVD PSMFQNMGGAGGAAPGAGPSA GARPSGSGPRVEEVD
T. brucei: hsp7OA4 .DD...NAVTT.VE...R..ND..-----E.SL..YNHRQ....G..A..L..M...M GGGDGP GGMP EGMP GGMP GGMP GGMG GGMG GAAAS...KV....
T. brucei: hsc70 ---EKLQ.RIAFLPHGK..VGTQL-----HTYTDAASIEA.VAKIERLAKRALKSARREGKDGWAPGNEDNGSGDDNDGDDNSDEEDELQRGRGVTEGSGRSPIRKRDRIEAINANTE
T. cruzi: hsp7OA8 IE....KT.TS.VE......NN..-----E.S...YEHRQ....NL.T..MTNM...M AGAGMA GGMP GGMS [GGMP]8 GG.NP--SSS...EV....
L. major: hsp70.4 ITQG.RQQ.Q..VNA.SS..EK.R-----EAT...YMEQT.LI.GIAHRFCRSSTKAC HGGPTKRWAEGRCPLR
L. amazonensis: A .DDT..STLNKE.EA.....S...-----EAT...YEH.Q....N..N..MTKM..SM GGGA GGMA GMPD MSGM SG.RPA..AS...KV....
L. amazonensis: C .DDT..STLNKE.EA.....S...-----EAT...YEH.Q....N..N..MTKM..SM GGGA GGMA GMPD MSSM SG.RPA..AS...KV....
L. donovani: hsc70 .DDS..ATLNKE.DVT....S...-----EATKEEYEH.Q....S..N..MT.M..SM GGAG GGMP GGMPDMSGMSG G.GPA..AS...KV....
L. brazil.: hsp70 .E.S.RSALNL..DT.....N...-----EAS...YEHRQ....ST.N..MT.M..SM GGGA GGMP GGMPDMSGMGG G.GPAA.AS...KV....
Theileria: .GADEVDSALST.TD..K.VEA..-----L..HD.YED.L.HV.G..N.LVT....SGAAP GGPD MGAG FP GG.-APPPQS....V....
Theileria parva .GADEVDNALNT.T...K.VET..-----L..HD..ED.L.HV.G..N.LVT....SGGAP GAGPDMGAG FP GGAPPPSSSSGPTVEEVD
Plasmodium:hsp7OA IPKD.S..CMKTVKSV.D..EK..-----...T..YNE.E.DT.F
Plasmodium:hsp7OA1 .QP.EI.TCMKT.TTI....EK..-----L.G.D.YE..Q..A.S..A..M..I..D.AGAA GGMP GGMP GGMP GGMN FP GGMP GAGMP GNAP AG....V...AEF
Plasmodium:hsp7OA1* .QP.EI.TCMKT.TTI....EK..-----L.G.D.YE..Q..A.S..A..M..I..DRAGAA GGMP GGMP GGMP GGMP GGMN FP GGMP GAGMP GNAP AG....V....
Plasmodium:hsp7OA2 .QP.EI.TCMKT.TTI....EK..-----L.G.D.YE..Q..A.S..A..M..I..D.AGAA GGMP GGMP GGMP GGMP GGMN FP GGMP GAGMP GNAP AG....V....
P.chabaudi:hsp70 .QPAEI.TCMKSVTSI....EK..-----L.G.D.YE..Q..A.A..A..M..I..D.AGAS GGMP GGMP GGMP GGMP GGMN FP GGMP GAGMP GN
P. cynmolgi: hsp70A2 .QPSEI.TCMKS.TTI....EK..-----L.S...YES.Q..A.S..A..M..I..DVGGAA [GGMP]6 GGMN FP GGMP GGGMP GGAP AG....V....
P. falcip.: hsp70A .QP.EI.TCMKT.TTI....EK..-----L.G.D.YE..Q..A.S..A..M..I..D.-AGAA [GGMP]4 SGMP GGMN FP GGMPG AGMP GNAPAG....V....
Eimeria: hsc70 I.AE.R.AATS..QK..D...K..-----L......E..Q..V.A..T..VT.M..S. AGAQ GGMP GGMP DMS-AAAGAAG...V....
Euplotes eurystomus FTDD..KT.NN..DD.KS....HP-----D..TSDYESQQ.K..AKFN..MM.V..SG DGRMP GGMP GGMP GGMY NNASAPAGADDLD
Cryptosporidium par. ..QSEIDEA.KK.KD..D..EH..-----....D..EHQQ..I.THMN.LMM.I.SAE [GGMP]11 GSNGPTVEEVD
Babesia microti ..QS.I.NAMKTLQNTFT.IE...-----L.D....E..L..VQA..T.LTA.I..SG GAGG APPGG FPGGG FP GG GSSGPTVEEVD
Chlamy.: hsc70: ..AS...SM.K.LTA.MD..EA..-----M..V...EHHL....GL.N...TR....G AGAGGMPGGGAGAGAA PS..SGA..KI....
Bremia: hsp7O IP.D..KA...KVT.TIN...A..-----S.....YEG.Q....GIAN..LQ.M.AA. GAA [GGMP]2 GMPGGMP GMPGGMP GMP [GGMP]3 GGWP PCWSSDQ..KI....
Achlya: hsc70 ID.S..KV.D.KVTDIIN...H..-----S......E..Q....GIAN..MQ.M.AAA GGAP GGMP GGMP DM GGAG AP-PPASHAQ..KI....
Pyrenomonas: hsp70 IQ.E..KS..EKVK.V..FIET.E-----DL....YEE.E...KNFAN........QG SVPD MGNF ST G.NEQDDNANM..KI...G
Ascophyllum nodosum IDGS...TLSG..EA.IS.....P-----S...A.YDSQREK..G.AN..M....A.. GGA GGMP GGMP GGMP GGFP GGAP AGGANAANDDGPSIEEVD
Guillardia th. hsc70 .IDS..SSLTSK.D.TIS..E..A-----N..V...E.NE.A..G.AM..MT.....G RM PDMG GGMPDMS GGAP SSGAGGAGGPHIEEVD
carrot: hsp70: .DAGD.....T.VN..I...EK..-----L..VD.LED.L....GL.N...AR....R GDVPI GGP GDMP GGGYG GS R.SSGA..KI....
spinach: hsc70 ......K...E..DASIQ...G..-----L..AD..DD.M....SI.N...A.M.... GG DMGGGMEDEG PTS.GGA..KI..CRLSCHFF
petunia: hsp7O ..A...KR.....D..IK...N..-----L..AD..ED.M....SI.N...A.M...G AG GATM DEDG PSVGGSAGSQTGA..KI....
tomato: hsc70A ..SD..K.....VDQ.IS..E...-----L..VD..ED.M....GI.N...A.M.... GG DAGVPMDD D.PPS..SSA..KI....
tomato: hsc70B ..AD.RT......EQ.IQ..DG..-----L..A...ED.M....SL.N...A.M.... GG DMDDE GP.-PS..G-A..KI....
Arabidopsis: 1 .PA...K....S.EQ.IQ..EG..-----L..AD..ED.M....SI.N...A.M.... GGEA GGPG ASGMD DD.-PPASGGA..KI....
soybean: hsp70 ..PDE.Q...K.VED.IQ..EG..-----M..VD..ED.Q....GI.N...A.M.... AG P GG DVPMGADMPA AGA..KI....
tobacco: hsp70 .DPS..Q...K.VD.TID...G..-----LV.VD..ED.L.D..NL.N...A.M...G ADGDVPMGGSADTGAGY GK.-GSTNNGA..KI....
maize: hsp7OA1 .PAE..K.....VDG.IS......-----L..V...ED.M....GI.N...A.M.?.E GA GMGA AAGM DEDA PS..SGA..KI....
lily: hsp70 .PAEV.K..D..MEG.IQ..EA..-----L..VD..DD.RR...GI.K.V.E..C..I GVIA DRMGMNEDGTASRT GGA..KI....
Lupinus p.: .PTE..K....TVDG.ISR..G..-----LP.V...ED.M....SL.N...A.M...VLAQMVLVLLIM .D.-PT.SGGA..KIG...
sea urchin: hsp70 K.PG...TVTK.VNDV.Q.....S-----L.D....TY.LE..QKT.S..MAKMHA.T GGPR GGG P.G-FPS.GAG...V....
sea urchin: hsp70-4 ..PG...TVTK.VNDV.Q.....S-----L.D....TY.LE..QKT.S..MA.MHAGT GGPR GGG P.G-FPS.GAG...V....
Trich. britovi mBiP? IPESDRKAVLNKCEEVLRWLETNQLAEKDEFEHKQKDLESLCNPICKCIRMRVNA SGMPNLQFLQVDLQVASSRGGGGGPTIEEVD
Dirofilaria: hsp70 I..D..K..QEKCD.TVR...G..-----....D..EHRQ....S..N...T....S. GGMP GGMP GGMP GGAP ----GG.STGG...I....
B. malayi: I..E..K..QEKCD.TVR...G..-----....D..EHRQ....S..N...T....S. GGMP GGMP GGMP GG APGA.STGG...I....
Brugia pahangi: I..E..K..QEKCD.TVR...G..-----....D..EHRQ....S..N...T....S. GGMP GGMP GGMP GG APGAGSTGGGPTIEEVD
O.volvulus: I.EF
C. elegans: hsp7OA I.PE..K....KCD.I.K......-----.......ESQQ.D..GLAK.DL.....S. GGAP PGAA PGGA A..A-G...I....
Dros.: hsc70-4 I.DS.RTT.L.KCN.TIK...A..-----L.D...YEHRQ....G..N...T...... GFPP GGMP GGP GGMP GAAG AAG-AA.AGGA...I....
Dros.: hsc70-1 F.P..R.TIQQRSS.TIA...A..-----L..RQ..EH.QQ...RI.S...TR..... GMAPPPTAGGSN-PGATGG....I....
mosquito: hsp70-1 L....RRTVQ.RCD.T.R.I.G.T-----M.....YEHQMQ..SR..S..MT..H.QA AGGP QPTSCG Q..-GGF.GRT...V....
mosquito: hsp70-3 L.D..RKTVQ.RCE.T.R.I.G.T-----M.D....EHKMQ..TKA.S..MT..H.QA AGGP SPSSCA Q..-GGF.GRT...V....
Dros.: hsp7OA2 .D....NSDL.KCNDTIR.....T-----.......DH.LE..TRH.S..MT.MH.Q.. GA GA GGPG AN CG Q..GGF..Y-..R.V....
Dros.: hsp7OA4 .D....NSVL.KCN.TIR.....T-----.......DH.ME..TRH.S..MT.MH.Q.. GA -A GGPG AN CG Q..GGF..Y-....V....
Schistosoma: IP.S.HQV.ISKCEDTIS...VH.-----S...H.YES.RE...K..A...T.V..A GGMP GGMH EASGAG..SGK...I....
S.japonicum: hsp70 I..S.RKNRILSKC.ETIRWMDNNQ----L......EE.KS...K..M...TAMNRAG GGVP SGMP GGMP GA--GGGGGKGPTIEEVD
Hydra m.: hsp70 I..E..KT.IKKCN.TV..V.K..-----T...DQYEH.QK...K..N...TK...AG GGMP GGMP GGMP GGMP GSGSKASSGGPTIEEVD
salamander: hsp70 I..D.RK..V.KCNQTIS.MEN..-----M.....YEHQQ....K..NS..T...Q GGMP GGMP SGSSGA ..-RQ.SSST...I....
rat: hsc7OA INDE..Q..L.KCN.IIS...K..-----.......EHQQ....K..N...T....S. GGMP GGMP GGFP GG G.PPS..AS....I....
mouse: hsc7OA INDE..Q..L.KCN.IIS...K..-----.......EHQQ....K..N...T....S. GGMP GGMP GGFP GG G.PPS..AS....I....
hamster: hsc7OA INDE..Q..L.KCN.IIS...K..-----.......EHQQ....K..N...T....S. GGMP GGMP GGFP GG G.PPS..AS....I....
cow: hsc70 INDE..Q..L.KCN.IIN...K..-----.......EHQQ....K..N...T....S. GGMP GGMP GGMP GGFP GG G.PPS..AS....I....
human: hsc7OA INDE..Q..L.KCN.IIN...K..-----.......EHQQ....K..N...T....S. GGMP GGMP GGFP GG G.PPS..AS....I....
mouse: hsc7OA* ..I....
mouse: pseudo1 INDE..Q..L.KCN.IIS...K..-----..Q....EHQQ....K..N...T.... .PPS..AS ..I....
human: pseudo1 INDE..H..LHKYN.IIN...K..-----.......EHQQ...KK./N...T....S. GGMP GGMP GGFP GG G.PPS..AS..F.I....
chicken: I.DQ..Q.VL.KCQ.VIS...R..-----M.....YEH.Q....KL.N..VT...... GG AG.---..SG-...I....
mouse: hsc7OB I..Q..N..L.KCQ.VIN...R..-----M...D.VEH.Q....R..N......... GPGG..SSG...I....
human: hsc70B I..Q..N..L.KCQ.VIN...R..-----M...D.YEH.Q....R..N........GG PGGG SGG-GGS.ASG...I....
cow: hsp70 I.DQ..N..L.KCQ.VIN...R..-----M...D.YEH.Q....R..N........-- GGPG GG-GGS.ASG...I....
Xenopus: hsp7OA1 I.DE..RT.SEKCTQVIS..EN..-----L.....YAFQQ.D..K......T....-- GGVP GGVP GSSCG A..--RQ.GN....I....
mouse: hsc70C I..S..K..L.KCN.V.S..EA..-----L...D..DH.R....NM.N...T....SG CTGPTCT PGY-TP.RAAT...I....
human: hsp70B IP.E.RR.MQ.KCR.V.A..EH..-----L.....YEHQKR...QI.R..F.R.. GG P GV P GGSS CG T..-RQ.DPST..II....
pig: hsp70 IP.E.RC.VQ.KCQ.VPYLWLEHNQ----L.....YEHQKR...QIASISQLLGPCTWGSSCGA QAPKGGPSTGPVIEEVD
pig: hsp70com: I.....KKVL.KCQ.VIS...A.T-----L...D..EH.R....Q..N....G..... GG P G P GGFG A-PDLK..SG....I....
mouse: hsp7OA1 ......K.VL.KCQ.VIS.....T-----L.D....VH.RE...R..S....G..... GA P GA GGFG A..-PK.ASG....I....
mouse: hsp70A2 ......K.VL.KCQ.VIS.....T-----L.D....VH.RE...R..S....G..... GA P GA GGFG A..PPK.ASG....I....
human: hsp7OA1 I.....K.VL.KCQ.VIS...A.T-----L...D..EH.R....Q..N....G..... GG P G P GGFG A.G-PK..SG....I....
human: hsp7OA2 I...H.K.VL.KCQ.VIS...A.T-----L...D..EH.R....Q..N....G?.... GG P G P GGFG A---PK..SG....I....
human: hsp7OA** I.....K.VL?KCQ.VIS.....T-----L...D..?H.R....Q..?....G..... GG P G ? GGFG A.GP?K..SG....I....
CONSENSUS LSEADKEKIEDAIXEALEWLDSNQ-----TAEKEEFKAKXKELEXVCQPIISKLYQGA------------------------------------------XQA-XXGGXXSGPTXEEVD-----------------
| |
Laurence (Larry) A. Moran [lamoran@utoronto.ca]
[Dept.
of Biochemistry][University of Toronto],
Toronto ON, Canada M5S 1A8